Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VN23_RS13665 Genome accession   NZ_CP014222
Coordinates   3040339..3041379 (-) Length   346 a.a.
NCBI ID   WP_046352384.1    Uniprot ID   -
Organism   Janthinobacterium sp. B9-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3035339..3046379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN23_RS13655 (VN23_13655) - 3037014..3038927 (-) 1914 WP_046352382.1 hypothetical protein -
  VN23_RS13660 (VN23_13660) pilU 3038936..3040072 (-) 1137 WP_046352383.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VN23_RS13665 (VN23_13665) pilT 3040339..3041379 (-) 1041 WP_046352384.1 type IV pilus twitching motility protein PilT Machinery gene
  VN23_RS13670 (VN23_13670) - 3041646..3042338 (+) 693 WP_046352385.1 YggS family pyridoxal phosphate-dependent enzyme -
  VN23_RS13675 (VN23_13675) proC 3042346..3043164 (+) 819 WP_046352386.1 pyrroline-5-carboxylate reductase -
  VN23_RS13680 (VN23_13680) - 3043174..3043767 (+) 594 WP_046352387.1 YggT family protein -
  VN23_RS13685 (VN23_13685) - 3043855..3044286 (-) 432 WP_046352388.1 FxsA family protein -
  VN23_RS13690 (VN23_13690) cutA 3044285..3044614 (+) 330 WP_046352389.1 divalent-cation tolerance protein CutA -
  VN23_RS13695 (VN23_13695) dsbD 3044611..3046143 (+) 1533 WP_046352390.1 protein-disulfide reductase DsbD -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38356.20 Da        Isoelectric Point: 6.7673

>NTDB_id=169550 VN23_RS13665 WP_046352384.1 3040339..3041379(-) (pilT) [Janthinobacterium sp. B9-8]
MEISELLAFTVKNKASDLHLSSGLPPMIRVDGDIRRINTPALSHKDVHDMVYDIMNDGQRKIYEDTLECDFSFEIPRLAR
FRVNAFVQNRGASAVFRVIPSDIKTLEELGCPRIFQDIAETPRGLVLVTGPTGSGKSTTLAAMINYINANEYGHILTVED
PIEFVHTSQKCLINQREVGPHTMSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKKKEGGRVAAHEVMIGIPAIRNLIRENKIAQMYSAIQTGSQFGMQTLDQCL
QGLLQRNIISLPEARMKAAIPDNFRG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=169550 VN23_RS13665 WP_046352384.1 3040339..3041379(-) (pilT) [Janthinobacterium sp. B9-8]
ATGGAAATCTCTGAATTATTAGCTTTCACCGTTAAAAACAAAGCATCAGACTTACATCTTTCGTCTGGTTTGCCTCCGAT
GATTCGGGTTGACGGGGATATTCGCCGGATTAATACGCCTGCGCTATCGCATAAAGACGTGCACGATATGGTGTACGACA
TCATGAACGACGGCCAGCGTAAGATTTACGAAGATACGCTGGAATGCGATTTTTCTTTCGAAATACCGCGTTTAGCACGT
TTCCGGGTGAATGCCTTTGTGCAAAATCGCGGGGCGAGTGCTGTTTTCCGGGTGATTCCATCGGATATCAAGACTCTGGA
AGAGCTAGGTTGCCCGCGTATTTTTCAAGACATTGCCGAAACACCGCGCGGCTTGGTGCTGGTGACCGGGCCAACAGGCT
CGGGTAAATCCACCACTTTGGCGGCGATGATCAACTATATCAATGCCAATGAATACGGCCATATCTTAACCGTGGAAGAC
CCGATTGAATTTGTCCACACCTCGCAAAAGTGCCTGATTAACCAGCGTGAAGTTGGCCCGCACACCATGAGCTTCTCTAA
TGCTTTGCGCTCTGCACTGCGTGAAGACCCTGACGTCATTCTGGTTGGGGAAATGCGCGATCTGGAAACCATCCGTCTGG
CGCTGACTGCCGCTGAAACCGGTCACTTAGTGTTTGGCACTTTGCATACCTCATCTGCGGCTAAAACCATTGACCGTGTG
GTGGATGTATTCCCTGCGGCAGAAAAAGAAATGGTGCGCTCGATGTTGTCTGAATCATTACGTGCCGTGATTTCACAAAC
GCTGCTGAAAAAGAAAGAAGGCGGGCGGGTGGCTGCGCATGAAGTGATGATCGGGATTCCGGCTATTCGTAACTTGATTC
GTGAAAATAAAATTGCGCAAATGTATTCGGCGATTCAAACCGGTTCGCAATTTGGGATGCAAACGCTGGATCAGTGTTTG
CAGGGCTTATTGCAGCGCAATATTATTTCCCTGCCTGAGGCCAGAATGAAAGCGGCTATTCCTGATAATTTCAGGGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.453

99.422

0.76
  pilT Acinetobacter baumannii D1279779

76.453

99.422

0.76
  pilT Acinetobacter baumannii strain A118

76.453

99.422

0.76
  pilT Pseudomonas stutzeri DSM 10701

75

99.422

0.746
  pilT Acinetobacter baylyi ADP1

74.709

99.422

0.743
  pilT Pseudomonas aeruginosa PAK

74.709

99.422

0.743
  pilT Legionella pneumophila strain Lp02

73.03

95.376

0.697
  pilT Legionella pneumophila strain ERS1305867

73.03

95.376

0.697
  pilT Neisseria meningitidis 8013

70.796

97.977

0.694
  pilT Neisseria gonorrhoeae MS11

70.501

97.977

0.691
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.399

0.662
  pilT Vibrio cholerae strain A1552

67.953

97.399

0.662
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.711

0.509
  pilU Vibrio cholerae strain A1552

42.197

100

0.422
  pilU Pseudomonas stutzeri DSM 10701

41.04

100

0.41
  pilU Acinetobacter baylyi ADP1

39.481

100

0.396

Multiple sequence alignment