Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VN23_RS13660 Genome accession   NZ_CP014222
Coordinates   3038936..3040072 (-) Length   378 a.a.
NCBI ID   WP_046352383.1    Uniprot ID   A0A0X8GQN8
Organism   Janthinobacterium sp. B9-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3033936..3045072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN23_RS13650 (VN23_13650) uvrA 3034093..3036954 (+) 2862 WP_046352381.1 excinuclease ABC subunit UvrA -
  VN23_RS13655 (VN23_13655) - 3037014..3038927 (-) 1914 WP_046352382.1 hypothetical protein -
  VN23_RS13660 (VN23_13660) pilU 3038936..3040072 (-) 1137 WP_046352383.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VN23_RS13665 (VN23_13665) pilT 3040339..3041379 (-) 1041 WP_046352384.1 type IV pilus twitching motility protein PilT Machinery gene
  VN23_RS13670 (VN23_13670) - 3041646..3042338 (+) 693 WP_046352385.1 YggS family pyridoxal phosphate-dependent enzyme -
  VN23_RS13675 (VN23_13675) proC 3042346..3043164 (+) 819 WP_046352386.1 pyrroline-5-carboxylate reductase -
  VN23_RS13680 (VN23_13680) - 3043174..3043767 (+) 594 WP_046352387.1 YggT family protein -
  VN23_RS13685 (VN23_13685) - 3043855..3044286 (-) 432 WP_046352388.1 FxsA family protein -
  VN23_RS13690 (VN23_13690) cutA 3044285..3044614 (+) 330 WP_046352389.1 divalent-cation tolerance protein CutA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42237.33 Da        Isoelectric Point: 6.4507

>NTDB_id=169549 VN23_RS13660 WP_046352383.1 3038936..3040072(-) (pilU) [Janthinobacterium sp. B9-8]
MEKEQAAKFMHDLLRHMRSKGASDLFITADFPPAMKIDGKVTPVSNQILTAQHSKELARAIMNDRQAEDFEGTKECNFAI
SPGQLGRFRVNAFMQQGQVGMVLRTINSEIPSLDDLGLPPVLRDIAMAKRGLVIFVGGTGSGKSTSLAAMIGHRNEHGYD
HIITIEDPIEYVHAHKNCIVTQREVGVDTDSWQAALKNTLRQAPDVILIGEIRDRETMDYAIAFSETGHLCMATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLRSFVSQRLIPHRTGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQALFDHYEAGKITYEDALRNADSVNDLRLSIKLQGSEAKHTDILSGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=169549 VN23_RS13660 WP_046352383.1 3038936..3040072(-) (pilU) [Janthinobacterium sp. B9-8]
ATGGAAAAGGAACAAGCCGCAAAATTTATGCATGATCTTTTGCGGCATATGCGCAGTAAAGGGGCCTCAGATTTATTTAT
TACGGCTGATTTTCCGCCTGCAATGAAGATTGACGGGAAAGTGACGCCGGTTTCAAATCAAATTCTGACGGCACAGCATA
GTAAGGAATTAGCGCGGGCGATTATGAATGATCGTCAGGCCGAAGATTTTGAAGGTACTAAAGAATGTAATTTTGCGATC
AGCCCCGGCCAGCTCGGGCGTTTTCGCGTGAATGCGTTTATGCAGCAGGGCCAGGTAGGCATGGTGCTGCGTACGATTAA
TTCAGAAATCCCCTCGCTGGATGATTTAGGCCTGCCGCCTGTATTACGCGATATTGCGATGGCAAAGCGGGGCTTGGTGA
TTTTTGTAGGCGGTACAGGTTCGGGTAAATCGACCTCGCTGGCAGCCATGATTGGCCACCGAAATGAGCATGGTTACGAT
CACATCATTACCATTGAAGACCCGATTGAATACGTTCACGCGCATAAAAATTGCATTGTGACCCAGCGTGAAGTGGGGGT
GGACACCGATAGCTGGCAGGCTGCACTGAAAAACACCCTGCGTCAGGCGCCAGATGTGATTTTGATTGGTGAAATTCGTG
ATCGCGAAACCATGGATTACGCAATTGCCTTCTCTGAAACCGGTCACCTTTGCATGGCGACTTTACACGCCAACTCGGCT
AATCAGGCGCTGGACCGGATTATCAACTTTTTCCCCGAAGAGCGTCGTCAGCAGCTCTTGATGGATTTATCTTTAAATCT
TCGCTCGTTTGTTTCTCAGCGGCTGATTCCGCATCGCACCGGCAAAGGCCGGGTGGCGGCTGTGGAAGTGATGCTAAATA
GCCCTTTGATTTCTGAATTGATTTTTAAAGGTGAAGTGCACGAAATCAAAGAAATCATGAAGAAATCGCGTGAGCTGGGA
ATGCAGACATTCGATCAGGCATTGTTTGATCATTATGAAGCAGGAAAAATCACTTACGAAGATGCTTTGCGCAATGCCGA
TTCAGTTAATGATTTACGATTGAGCATTAAGCTGCAAGGTTCAGAAGCGAAGCATACGGATATTCTTTCTGGTCTTGATC
ATCTTGATATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X8GQN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.533

92.857

0.59
  pilU Acinetobacter baylyi ADP1

59.331

94.974

0.563
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.373

88.624

0.402
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.624

0.373
  pilT Legionella pneumophila strain Lp02

44.164

83.862

0.37
  pilT Legionella pneumophila strain ERS1305867

44.164

83.862

0.37
  pilT Acinetobacter baumannii D1279779

42.296

87.566

0.37
  pilT Acinetobacter nosocomialis M2

42.296

87.566

0.37
  pilT Acinetobacter baumannii strain A118

42.296

87.566

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.492

83.333

0.362
  pilT Vibrio cholerae strain A1552

43.492

83.333

0.362
  pilT Acinetobacter baylyi ADP1

44.625

81.217

0.362

Multiple sequence alignment