Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACG33_RS01415 Genome accession   NZ_CP011971
Coordinates   331600..332640 (+) Length   346 a.a.
NCBI ID   WP_066918066.1    Uniprot ID   -
Organism   Steroidobacter denitrificans strain DSM 18526     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326600..337640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG33_RS01380 (ACG33_01370) - 326650..327276 (-) 627 WP_066918057.1 YbhB/YbcL family Raf kinase inhibitor-like protein -
  ACG33_RS01385 (ACG33_01375) - 327538..328065 (+) 528 WP_066918059.1 alpha/beta hydrolase -
  ACG33_RS01390 (ACG33_01380) - 328069..328521 (+) 453 WP_083536334.1 TerB family tellurite resistance protein -
  ACG33_RS01395 (ACG33_01385) - 328630..328941 (-) 312 WP_066918063.1 HU family DNA-binding protein -
  ACG33_RS01400 (ACG33_01390) - 329206..329739 (-) 534 WP_066922650.1 YggT family protein -
  ACG33_RS01405 (ACG33_01395) proC 329906..330688 (-) 783 WP_407696489.1 pyrroline-5-carboxylate reductase -
  ACG33_RS01410 (ACG33_01400) - 330805..331542 (-) 738 WP_066918065.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACG33_RS01415 (ACG33_01405) pilT 331600..332640 (+) 1041 WP_066918066.1 type IV pilus twitching motility protein PilT Machinery gene
  ACG33_RS01420 (ACG33_01410) pilU 332735..333898 (+) 1164 WP_066918067.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG33_RS01425 (ACG33_01415) pilU 333913..335034 (+) 1122 WP_237392661.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG33_RS01430 (ACG33_01420) - 335153..336043 (-) 891 WP_066918068.1 dihydroorotate dehydrogenase electron transfer subunit -
  ACG33_RS01435 (ACG33_01425) - 336040..337029 (-) 990 WP_066918069.1 aspartate carbamoyltransferase catalytic subunit -
  ACG33_RS01440 (ACG33_01430) ruvX 337051..337464 (-) 414 WP_066918074.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38497.37 Da        Isoelectric Point: 7.2234

>NTDB_id=149762 ACG33_RS01415 WP_066918066.1 331600..332640(+) (pilT) [Steroidobacter denitrificans strain DSM 18526]
MSVDIAQLLTFAVKNNASDLHLSAGVPPMIRVDGDMKRINMPALTHKEVHSMVYDIMNDKQRKAYEEFYETDFSFEIPKL
ARFRVNAFNQSRGAAAVFRTIPSLIMSLDDLNAPKIFKDLSMQPRGLVLVTGPTGSGKSTTLAAMVNYVNENKPDHIITI
EDPIEFVHECKRSLVNQREVHRDTLGFSEALRSALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTID
RIVDVFPAAEKEMVRTMLSESLRAAISQTLLKRIGGGRVAAHEIMIGTPAIRNLIREGKIAQMYSAIQTGQKDGMQTLDQ
CLTELLQKGIVTKEEARFRAQNKDAF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=149762 ACG33_RS01415 WP_066918066.1 331600..332640(+) (pilT) [Steroidobacter denitrificans strain DSM 18526]
ATGTCCGTCGACATCGCGCAACTGCTGACATTCGCAGTCAAGAACAACGCATCTGATCTGCATTTGTCCGCAGGTGTACC
GCCCATGATACGTGTGGACGGCGACATGAAGCGGATCAATATGCCCGCGTTGACGCACAAGGAAGTGCACAGCATGGTGT
ACGACATCATGAATGACAAGCAAAGGAAGGCCTACGAGGAATTCTACGAAACCGATTTCTCATTCGAAATCCCCAAGCTG
GCCCGTTTTCGCGTCAATGCGTTCAATCAGAGTCGTGGCGCCGCGGCCGTGTTCCGGACGATCCCGTCGCTGATCATGTC
GCTGGATGACTTGAATGCTCCGAAGATATTCAAGGACCTGTCGATGCAGCCTCGCGGACTGGTGCTGGTGACCGGTCCGA
CCGGCTCCGGCAAGTCGACGACCCTGGCTGCGATGGTCAACTATGTCAATGAAAACAAGCCCGACCATATCATCACGATC
GAGGATCCGATCGAGTTTGTGCATGAGTGTAAGCGTTCACTGGTGAACCAGCGCGAGGTGCATCGCGACACGCTTGGCTT
CAGCGAAGCGCTGCGTTCGGCCCTGCGCGAAGATCCCGACGTGGTGCTGGTGGGCGAAATGCGTGATCTGGAGACCATCC
GCCTGGCGCTTACTGCGGCGGAAACCGGGCATCTGGTGTTCGGTACGCTGCATACGAGTTCGGCCGCCAAGACGATCGAC
CGTATCGTCGATGTCTTCCCTGCGGCAGAGAAGGAGATGGTACGAACGATGCTGTCGGAATCGCTGCGGGCAGCGATTTC
GCAGACGTTGCTCAAGCGCATCGGCGGCGGGCGTGTCGCAGCGCACGAAATCATGATCGGTACGCCTGCCATTCGCAATC
TGATCCGTGAAGGCAAGATTGCACAGATGTATTCGGCGATTCAGACGGGCCAGAAGGACGGCATGCAGACGCTGGACCAG
TGTCTGACGGAATTATTACAGAAGGGTATCGTTACGAAAGAGGAGGCGCGCTTCCGCGCCCAGAACAAGGATGCATTCTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

74.709

99.422

0.743
  pilT Acinetobacter baylyi ADP1

74.419

99.422

0.74
  pilT Pseudomonas stutzeri DSM 10701

73.837

99.422

0.734
  pilT Acinetobacter baumannii strain A118

73.547

99.422

0.731
  pilT Acinetobacter nosocomialis M2

73.547

99.422

0.731
  pilT Acinetobacter baumannii D1279779

73.547

99.422

0.731
  pilT Legionella pneumophila strain Lp02

71.802

99.422

0.714
  pilT Legionella pneumophila strain ERS1305867

71.802

99.422

0.714
  pilT Neisseria meningitidis 8013

67.536

99.711

0.673
  pilT Neisseria gonorrhoeae MS11

67.246

99.711

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.358

96.821

0.662
  pilT Vibrio cholerae strain A1552

68.358

96.821

0.662
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

100

0.494
  pilU Pseudomonas stutzeri DSM 10701

40.525

99.133

0.402
  pilU Acinetobacter baylyi ADP1

41.231

93.931

0.387
  pilU Vibrio cholerae strain A1552

39.104

96.821

0.379

Multiple sequence alignment