Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACG33_RS01420 Genome accession   NZ_CP011971
Coordinates   332735..333898 (+) Length   387 a.a.
NCBI ID   WP_066918067.1    Uniprot ID   A0A127F5R4
Organism   Steroidobacter denitrificans strain DSM 18526     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327735..338898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG33_RS01390 (ACG33_01380) - 328069..328521 (+) 453 WP_083536334.1 TerB family tellurite resistance protein -
  ACG33_RS01395 (ACG33_01385) - 328630..328941 (-) 312 WP_066918063.1 HU family DNA-binding protein -
  ACG33_RS01400 (ACG33_01390) - 329206..329739 (-) 534 WP_066922650.1 YggT family protein -
  ACG33_RS01405 (ACG33_01395) proC 329906..330688 (-) 783 WP_407696489.1 pyrroline-5-carboxylate reductase -
  ACG33_RS01410 (ACG33_01400) - 330805..331542 (-) 738 WP_066918065.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACG33_RS01415 (ACG33_01405) pilT 331600..332640 (+) 1041 WP_066918066.1 type IV pilus twitching motility protein PilT Machinery gene
  ACG33_RS01420 (ACG33_01410) pilU 332735..333898 (+) 1164 WP_066918067.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG33_RS01425 (ACG33_01415) pilU 333913..335034 (+) 1122 WP_237392661.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG33_RS01430 (ACG33_01420) - 335153..336043 (-) 891 WP_066918068.1 dihydroorotate dehydrogenase electron transfer subunit -
  ACG33_RS01435 (ACG33_01425) - 336040..337029 (-) 990 WP_066918069.1 aspartate carbamoyltransferase catalytic subunit -
  ACG33_RS01440 (ACG33_01430) ruvX 337051..337464 (-) 414 WP_066918074.1 Holliday junction resolvase RuvX -
  ACG33_RS01445 (ACG33_01435) - 337515..338078 (-) 564 WP_066918081.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43390.05 Da        Isoelectric Point: 6.4080

>NTDB_id=149763 ACG33_RS01420 WP_066918067.1 332735..333898(+) (pilU) [Steroidobacter denitrificans strain DSM 18526]
MERDQAIKLMQDLLRRLVERKGSDLFVTAGFPPAIKVDGEIRPQSERVLTPEQAAVLVRSIMNDKQTLEFDATKECNFAI
APVGIGRFRVSAFVQQSLLGCVVRTINAKIPTLEEMQLPPILKDIVMTKRGLVIVVGGTGSGKSTSLAAMLNYRNEKSRG
HIITIEDPVEYVHNHKGCVVTHREVGVDTENWHTALKNTLRQAPDVILIGEIRDRETMEYGIQFAETGHLVLATLHANSA
NQALDRIINFFPEERREQLLMDLSLNTRALISQRLIPRDSGTGRIAAMEIMLNSPLITDLIFKGEVAAIKEVMARSNRLG
MQTFDQALFELYEAGLISYEDALRNADSKNEVRLRIKLESKREMKIADEASNSLRIMEEETEGVLGR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=149763 ACG33_RS01420 WP_066918067.1 332735..333898(+) (pilU) [Steroidobacter denitrificans strain DSM 18526]
ATGGAACGCGATCAAGCCATTAAGCTGATGCAGGACCTGCTGCGGCGTCTCGTGGAGCGCAAGGGTTCCGATCTGTTCGT
GACGGCGGGATTTCCGCCGGCGATCAAGGTGGACGGTGAAATTCGTCCCCAGTCTGAGCGGGTGTTGACGCCCGAGCAGG
CGGCGGTGCTGGTGCGTTCGATCATGAACGATAAACAGACCCTGGAATTCGATGCGACCAAGGAGTGCAATTTCGCGATT
GCCCCCGTGGGCATCGGCCGCTTTCGTGTCAGCGCCTTCGTACAGCAGAGCCTGCTCGGCTGCGTGGTGCGTACGATCAA
CGCGAAAATTCCGACGCTCGAGGAAATGCAGCTGCCGCCGATTCTGAAGGACATCGTCATGACCAAACGCGGCCTGGTCA
TCGTGGTCGGCGGCACCGGTTCCGGCAAATCCACGTCGCTGGCGGCGATGCTCAACTATCGCAACGAAAAAAGCCGCGGC
CACATCATCACGATCGAGGATCCGGTCGAGTACGTCCATAATCACAAGGGCTGTGTCGTCACGCATCGCGAGGTAGGCGT
GGACACCGAGAACTGGCATACGGCACTCAAGAATACGCTGCGCCAGGCGCCCGATGTGATCCTGATCGGCGAAATCCGGG
ATCGGGAGACCATGGAATACGGTATCCAGTTCGCCGAGACCGGCCACCTGGTACTGGCGACCCTGCATGCGAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAATTTTTTCCCGGAGGAGCGCCGCGAGCAACTGCTCATGGATCTGTCGCTCAATAC
GCGCGCCCTGATTTCCCAGCGTCTGATACCGCGCGACTCGGGTACGGGCCGTATCGCGGCCATGGAGATCATGTTGAACT
CGCCCTTGATCACCGATCTGATCTTCAAGGGCGAAGTCGCCGCAATCAAGGAAGTCATGGCAAGGTCCAACCGATTGGGC
ATGCAGACCTTCGACCAGGCGTTGTTCGAGTTATACGAAGCCGGTCTCATTTCCTATGAGGATGCCCTGCGCAATGCGGA
TTCCAAGAACGAGGTGCGGCTGCGCATCAAGCTGGAAAGCAAGCGCGAGATGAAAATTGCCGATGAGGCGAGCAATTCTC
TGCGTATCATGGAGGAAGAGACCGAAGGCGTGCTTGGGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127F5R4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.322

91.473

0.543
  pilU Acinetobacter baylyi ADP1

58

90.439

0.525
  pilU Vibrio cholerae strain A1552

55.132

88.114

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

89.406

0.385
  pilT Pseudomonas aeruginosa PAK

44.214

87.08

0.385
  pilT Acinetobacter baumannii strain A118

44.144

86.047

0.38
  pilT Acinetobacter nosocomialis M2

44.144

86.047

0.38
  pilT Acinetobacter baumannii D1279779

44.144

86.047

0.38
  pilT Acinetobacter baylyi ADP1

43.544

86.047

0.375
  pilT Pseudomonas stutzeri DSM 10701

43.027

87.08

0.375
  pilT Legionella pneumophila strain Lp02

41.888

87.597

0.367
  pilT Legionella pneumophila strain ERS1305867

41.888

87.597

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

85.788

0.364
  pilT Vibrio cholerae strain A1552

42.47

85.788

0.364

Multiple sequence alignment