Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACG33_RS01425 Genome accession   NZ_CP011971
Coordinates   333913..335034 (+) Length   373 a.a.
NCBI ID   WP_237392661.1    Uniprot ID   -
Organism   Steroidobacter denitrificans strain DSM 18526     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328913..340034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG33_RS01400 (ACG33_01390) - 329206..329739 (-) 534 WP_066922650.1 YggT family protein -
  ACG33_RS01405 (ACG33_01395) proC 329906..330688 (-) 783 WP_407696489.1 pyrroline-5-carboxylate reductase -
  ACG33_RS01410 (ACG33_01400) - 330805..331542 (-) 738 WP_066918065.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACG33_RS01415 (ACG33_01405) pilT 331600..332640 (+) 1041 WP_066918066.1 type IV pilus twitching motility protein PilT Machinery gene
  ACG33_RS01420 (ACG33_01410) pilU 332735..333898 (+) 1164 WP_066918067.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG33_RS01425 (ACG33_01415) pilU 333913..335034 (+) 1122 WP_237392661.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG33_RS01430 (ACG33_01420) - 335153..336043 (-) 891 WP_066918068.1 dihydroorotate dehydrogenase electron transfer subunit -
  ACG33_RS01435 (ACG33_01425) - 336040..337029 (-) 990 WP_066918069.1 aspartate carbamoyltransferase catalytic subunit -
  ACG33_RS01440 (ACG33_01430) ruvX 337051..337464 (-) 414 WP_066918074.1 Holliday junction resolvase RuvX -
  ACG33_RS01445 (ACG33_01435) - 337515..338078 (-) 564 WP_066918081.1 YqgE/AlgH family protein -
  ACG33_RS01450 (ACG33_01440) - 338147..339067 (-) 921 WP_066918083.1 energy transducer TonB -
  ACG33_RS01455 (ACG33_01445) gshB 339079..340026 (-) 948 WP_066918085.1 glutathione synthase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41891.27 Da        Isoelectric Point: 6.4261

>NTDB_id=149764 ACG33_RS01425 WP_237392661.1 333913..335034(+) (pilU) [Steroidobacter denitrificans strain DSM 18526]
MSADGDVLENESDVTGEERQVMLNTKPLFKLMVDKRASDLFFTSYAPIKIKIEGQIFPVNKQMLTPEVVRQAAYGLMSQE
QIEAFNEELEIDFAISEPGLGRFRINVFHQRGNPAMVLRYITADMPRLDDLGLPEILKDLMMLKRGLLLMVGATGSGKST
TLASMINYRNEHSSDHIITIEDPIEFLHSNKKSIVNQREVGLDTKSYARALRSAMRAAPDVLLIGEIRDRETMESAIMLS
GTGHLVLATLHANNAAETLDRIINMFPRDQHTQIFLDLSQYLRAIMAQRLVPGKNKRRVAAVELLINTPHVQELIKKGDV
LGAKEALRTSSEKGMQHFDTALYELYRAGRITMEDALAYADSRTNLEAKINFG

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=149764 ACG33_RS01425 WP_237392661.1 333913..335034(+) (pilU) [Steroidobacter denitrificans strain DSM 18526]
ATGTCGGCCGATGGCGACGTGCTCGAGAATGAAAGCGACGTGACCGGCGAGGAGCGCCAGGTCATGCTCAATACCAAGCC
GTTGTTCAAGTTGATGGTGGACAAACGGGCTTCGGATCTGTTCTTTACTTCCTACGCACCTATCAAGATCAAGATCGAAG
GGCAGATATTCCCGGTCAACAAGCAGATGCTGACGCCGGAAGTCGTGCGCCAAGCGGCGTATGGTCTCATGAGCCAGGAG
CAGATCGAGGCTTTCAACGAGGAACTGGAGATCGATTTCGCGATTTCCGAGCCGGGTTTAGGCCGTTTTCGCATCAACGT
GTTCCATCAGCGCGGCAATCCGGCCATGGTGTTGCGCTATATCACCGCCGATATGCCGCGCCTGGACGACTTGGGGCTGC
CCGAGATCCTGAAGGATCTGATGATGCTCAAGCGCGGCCTGCTGCTGATGGTCGGCGCGACCGGTTCGGGCAAATCGACC
ACGCTGGCCTCGATGATCAATTATCGCAACGAGCATTCCTCGGATCACATCATCACGATCGAGGATCCGATCGAATTTCT
GCACTCCAACAAGAAATCCATCGTCAATCAACGCGAAGTCGGCCTGGACACGAAATCCTATGCACGCGCGCTGCGCAGCG
CCATGCGCGCGGCGCCCGATGTGTTGCTGATCGGCGAAATCCGCGACCGTGAAACGATGGAGTCGGCGATCATGTTGTCG
GGTACGGGACACCTGGTGCTTGCCACCTTGCATGCCAACAATGCAGCCGAAACGCTGGATCGCATCATCAACATGTTTCC
GCGCGACCAGCACACGCAGATCTTCCTGGATCTTTCCCAGTACCTGCGGGCGATCATGGCGCAGCGCCTGGTGCCGGGCA
AGAACAAGCGGCGCGTCGCTGCCGTGGAGTTGTTGATCAATACTCCGCATGTGCAGGAGCTGATCAAGAAAGGCGATGTG
CTCGGTGCGAAGGAGGCGCTGCGCACCAGCAGCGAGAAGGGCATGCAGCATTTCGATACGGCATTGTACGAGTTGTACCG
CGCCGGACGCATCACCATGGAAGACGCACTCGCCTACGCCGATTCACGCACCAACCTGGAAGCCAAGATCAATTTCGGCT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(33-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.582

93.566

0.501
  pilU Acinetobacter baylyi ADP1

49.713

93.298

0.464
  pilU Vibrio cholerae strain A1552

46.131

90.08

0.416
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.515

89.544

0.381
  pilT Acinetobacter baylyi ADP1

41.493

89.812

0.373
  pilT Neisseria meningitidis 8013

40.909

88.472

0.362

Multiple sequence alignment