Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IMCC21906_RS01195 Genome accession   NZ_CP011477
Coordinates   267748..268782 (-) Length   344 a.a.
NCBI ID   WP_047010636.1    Uniprot ID   A0A0F7LXA3
Organism   Spongiibacter sp. IMCC21906     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 262748..273782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMCC21906_RS01165 (IMCC21906_00236) pilG 262821..263207 (-) 387 WP_047010631.1 twitching motility response regulator PilG Regulator
  IMCC21906_RS01170 (IMCC21906_00237) gshB 263492..264457 (+) 966 WP_047010632.1 glutathione synthase -
  IMCC21906_RS01175 (IMCC21906_00238) - 264457..265353 (+) 897 WP_047010633.1 energy transducer TonB -
  IMCC21906_RS01180 (IMCC21906_00239) - 265518..266084 (+) 567 WP_047010634.1 YqgE/AlgH family protein -
  IMCC21906_RS01185 (IMCC21906_00240) ruvX 266097..266534 (+) 438 WP_047013027.1 Holliday junction resolvase RuvX -
  IMCC21906_RS01190 (IMCC21906_00241) pilU 266543..267712 (-) 1170 WP_047010635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IMCC21906_RS01195 (IMCC21906_00242) pilT 267748..268782 (-) 1035 WP_047010636.1 type IV pilus twitching motility protein PilT Machinery gene
  IMCC21906_RS01200 (IMCC21906_00243) - 268962..269657 (+) 696 WP_047010637.1 YggS family pyridoxal phosphate-dependent enzyme -
  IMCC21906_RS01205 (IMCC21906_00244) proC 269803..270636 (+) 834 WP_197085983.1 pyrroline-5-carboxylate reductase -
  IMCC21906_RS01210 (IMCC21906_00245) - 270636..271223 (+) 588 WP_047010639.1 YggT family protein -
  IMCC21906_RS01215 (IMCC21906_00246) - 271248..271547 (+) 300 WP_047010640.1 DUF167 family protein -
  IMCC21906_RS01220 (IMCC21906_00247) - 271585..272760 (+) 1176 WP_047010641.1 homoserine O-acetyltransferase -
  IMCC21906_RS01225 (IMCC21906_00248) metW 272750..273340 (+) 591 WP_047010642.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38315.94 Da        Isoelectric Point: 6.3497

>NTDB_id=146158 IMCC21906_RS01195 WP_047010636.1 267748..268782(-) (pilT) [Spongiibacter sp. IMCC21906]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPPLEHREVHSLIYEIMNDRQRRDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLGMGKVFKDVSMLPRGLVLVTGPTGSGKSTTLAAMIDYINDNKFEHILTIED
PIEFVHDSKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGEEKAMVRSMLSESLQAVISQTLIKKPEGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQSGANVGMQTIDQCL
KNLVESRQISRDAAKLKARFPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=146158 IMCC21906_RS01195 WP_047010636.1 267748..268782(-) (pilT) [Spongiibacter sp. IMCC21906]
ATGGATATTACTGAATTGCTGGCCTTTAGTGCCAAGCAAAATGCGTCAGATTTGCATTTGTCGGCGGGCCTGCCGCCGAT
GATCCGGGTAGATGGTGATGTGCGGCGAATTAATCTGCCGCCGTTGGAGCATCGGGAAGTTCATTCGCTTATTTACGAGA
TTATGAATGATCGCCAGCGCCGAGACTTTGAAGAGTTTCTGGAAACGGATTTCTCTTTTGAAGTGCCGGGGGTTGCCCGT
TTCCGGGTCAATGCTTTTAACCAAAATCGGGGTGCCGGTGCGGTATTCAGAACCATCCCTTCCAAGGTGTTAACCCTTGA
AGACTTGGGCATGGGCAAGGTATTTAAAGACGTATCGATGTTGCCCCGAGGTCTGGTGCTGGTGACGGGGCCTACTGGCT
CAGGTAAGTCCACGACCTTGGCGGCGATGATCGACTATATCAACGACAATAAATTTGAGCATATTCTTACTATAGAAGAC
CCTATTGAATTTGTTCACGACTCCAAAAAATGCCTGGTTAACCAGCGTGAAGTTCACCGCGACACCCACGGTTTTAGCGA
AGCGCTGCGTTCGGCGCTGCGGGAAGATCCCGATATTATCTTGGTGGGTGAGCTGCGGGACTTGGAAACGATTCGCTTGG
CGCTGACGGCTGCCGAAACGGGGCATTTGGTATTTGGCACCTTGCACACTACCTCGGCGGCAAAAACGATTGACCGGGTA
GTTGATGTGTTTCCCGGTGAAGAAAAAGCCATGGTACGGTCGATGTTGTCCGAATCGCTGCAGGCGGTTATTTCTCAAAC
CTTGATTAAAAAGCCGGAAGGGGGACGGGTTGCCGCCCATGAAATTATGATTGGTACACCGGCAATTCGTAATTTAATTC
GAGAAGACAAGGTCGCCCAGATGTATTCGGCCATTCAGTCGGGTGCCAATGTCGGTATGCAGACCATAGACCAGTGCTTG
AAGAATCTAGTGGAGTCTCGTCAAATTAGCCGAGACGCAGCCAAGCTGAAAGCGCGGTTCCCGGAAAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7LXA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Pseudomonas stutzeri DSM 10701

85.465

100

0.855
  pilT Acinetobacter nosocomialis M2

81.977

100

0.82
  pilT Acinetobacter baumannii D1279779

81.977

100

0.82
  pilT Acinetobacter baumannii strain A118

81.977

100

0.82
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

74.251

97.093

0.721
  pilT Legionella pneumophila strain ERS1305867

74.251

97.093

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.988

94.186

0.669
  pilT Vibrio cholerae strain A1552

70.988

94.186

0.669
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Neisseria meningitidis 8013

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

41.888

98.547

0.413
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment