Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IMCC21906_RS01190 Genome accession   NZ_CP011477
Coordinates   266543..267712 (-) Length   389 a.a.
NCBI ID   WP_047010635.1    Uniprot ID   A0A0F7M0S9
Organism   Spongiibacter sp. IMCC21906     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261543..272712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMCC21906_RS01155 (IMCC21906_00234) - 261813..262349 (-) 537 WP_047010629.1 chemotaxis protein CheW -
  IMCC21906_RS01160 (IMCC21906_00235) - 262357..262719 (-) 363 WP_047010630.1 PleD family two-component system response regulator -
  IMCC21906_RS01165 (IMCC21906_00236) pilG 262821..263207 (-) 387 WP_047010631.1 twitching motility response regulator PilG Regulator
  IMCC21906_RS01170 (IMCC21906_00237) gshB 263492..264457 (+) 966 WP_047010632.1 glutathione synthase -
  IMCC21906_RS01175 (IMCC21906_00238) - 264457..265353 (+) 897 WP_047010633.1 energy transducer TonB -
  IMCC21906_RS01180 (IMCC21906_00239) - 265518..266084 (+) 567 WP_047010634.1 YqgE/AlgH family protein -
  IMCC21906_RS01185 (IMCC21906_00240) ruvX 266097..266534 (+) 438 WP_047013027.1 Holliday junction resolvase RuvX -
  IMCC21906_RS01190 (IMCC21906_00241) pilU 266543..267712 (-) 1170 WP_047010635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IMCC21906_RS01195 (IMCC21906_00242) pilT 267748..268782 (-) 1035 WP_047010636.1 type IV pilus twitching motility protein PilT Machinery gene
  IMCC21906_RS01200 (IMCC21906_00243) - 268962..269657 (+) 696 WP_047010637.1 YggS family pyridoxal phosphate-dependent enzyme -
  IMCC21906_RS01205 (IMCC21906_00244) proC 269803..270636 (+) 834 WP_197085983.1 pyrroline-5-carboxylate reductase -
  IMCC21906_RS01210 (IMCC21906_00245) - 270636..271223 (+) 588 WP_047010639.1 YggT family protein -
  IMCC21906_RS01215 (IMCC21906_00246) - 271248..271547 (+) 300 WP_047010640.1 DUF167 family protein -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 43093.16 Da        Isoelectric Point: 6.6597

>NTDB_id=146157 IMCC21906_RS01190 WP_047010635.1 266543..267712(-) (pilU) [Spongiibacter sp. IMCC21906]
MDIEKLLRIMQEKGASDLFISAGVPPSIKINGQIGPLSSNALSPEQTREVVMGVMTEQQRRDFVRDKECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETKIPTTDDLGLPDTIKDLAMSKRGLIIFVGATGTGKSTSLASMIGHRNNNSKGHIITVED
PIEFLHQHRGCIVTQREVGIDTESFETALKNMLRQAPDVIMIGEVRSRETMENAITFAETGHLCLCTLHANNANQALDRI
LHFFPAERHPQLWMDLSLNLRAMVAQQLVPTRSGDGRRACVEILLNTPLAADLIRKGDVAGLKGLMSQSTEHGMQTFDQA
LYELYSQGEITYEDAIAHADSANDLRLLIKLGADNRPDNINAMAGRLTMQDDSEDSRDNGNNGILGRRR

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=146157 IMCC21906_RS01190 WP_047010635.1 266543..267712(-) (pilU) [Spongiibacter sp. IMCC21906]
TTGGACATTGAAAAGCTGCTGCGAATTATGCAGGAGAAGGGTGCCTCAGATTTATTTATCTCGGCTGGGGTGCCGCCGTC
GATAAAAATTAATGGCCAGATTGGACCGCTCAGCAGCAATGCACTTAGTCCCGAGCAAACGCGTGAAGTGGTGATGGGGG
TGATGACCGAGCAGCAGCGCCGAGACTTTGTGCGGGACAAAGAATGTAATTTTGCCATCAGCGCCCGAGGGATTGGTCGT
TTTCGGGTCAGTGCATTTTATCAGCGCAATTTAGTGGGAATGGTGTTGCGGCGCATCGAGACCAAAATTCCCACCACGGA
TGATTTAGGTTTACCCGATACGATCAAAGACCTTGCCATGAGTAAGCGTGGTTTGATTATTTTTGTGGGTGCTACCGGTA
CCGGTAAGTCGACTTCCTTGGCGTCGATGATCGGTCACCGAAATAATAATTCCAAAGGTCATATCATTACGGTAGAAGAT
CCCATTGAGTTTCTTCACCAGCACCGTGGCTGCATTGTCACCCAGCGTGAAGTGGGGATTGATACCGAGTCCTTTGAAAC
CGCACTGAAAAATATGCTTCGCCAGGCCCCCGACGTGATTATGATCGGCGAGGTGCGTTCTCGCGAAACCATGGAAAATG
CGATTACCTTTGCCGAGACCGGGCATTTATGTCTGTGTACCCTGCACGCCAATAATGCTAACCAAGCCTTGGATCGAATT
CTGCATTTCTTCCCGGCGGAGCGGCACCCCCAGTTGTGGATGGATCTGTCGCTTAATCTGCGGGCTATGGTCGCACAGCA
GCTGGTGCCAACCCGTAGCGGTGATGGTCGCCGGGCTTGTGTTGAAATACTGTTGAATACGCCGCTGGCTGCCGACTTAA
TTCGTAAAGGGGATGTGGCGGGTTTAAAAGGGTTGATGAGCCAGTCAACTGAACACGGTATGCAGACTTTTGATCAAGCC
TTATATGAGCTGTATTCGCAGGGTGAAATTACTTACGAAGACGCGATTGCCCATGCTGATTCTGCCAATGATTTGCGCTT
GTTAATAAAGTTGGGGGCCGACAATCGCCCCGATAATATTAACGCCATGGCGGGGAGGTTAACGATGCAAGATGACAGTG
AGGATAGCCGTGACAACGGCAACAATGGCATCTTAGGGCGCCGCCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7M0S9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

72.267

96.401

0.697
  pilU Acinetobacter baylyi ADP1

62.67

94.344

0.591
  pilU Vibrio cholerae strain A1552

55.429

89.974

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.543

87.147

0.388

Multiple sequence alignment