Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   UIB01_RS04670 Genome accession   NZ_CP007509
Coordinates   1019210..1019632 (-) Length   140 a.a.
NCBI ID   WP_038657331.1    Uniprot ID   -
Organism   Stutzerimonas decontaminans strain 19SMN4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1014210..1024632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UIB01_RS04655 (UIB01_04720) - 1014801..1015559 (+) 759 WP_038657326.1 Nif3-like dinuclear metal center hexameric protein -
  UIB01_RS04660 (UIB01_04725) cysD 1015747..1016664 (+) 918 WP_038657327.1 sulfate adenylyltransferase subunit CysD -
  UIB01_RS04665 (UIB01_04730) cysN 1016676..1018574 (+) 1899 WP_038657329.1 sulfate adenylyltransferase subunit CysN -
  UIB01_RS04670 (UIB01_04735) pilA 1019210..1019632 (-) 423 WP_038657331.1 pilin Machinery gene
  UIB01_RS04675 (UIB01_04740) pilB 1019954..1021666 (+) 1713 WP_038657333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  UIB01_RS04680 (UIB01_04745) pilC 1021669..1022889 (+) 1221 WP_038657335.1 type II secretion system F family protein Machinery gene
  UIB01_RS04685 (UIB01_04750) pilD 1022890..1023759 (+) 870 WP_038657337.1 A24 family peptidase Machinery gene
  UIB01_RS04690 (UIB01_04755) coaE 1023911..1024519 (+) 609 WP_038657340.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14806.83 Da        Isoelectric Point: 4.3603

>NTDB_id=120132 UIB01_RS04670 WP_038657331.1 1019210..1019632(-) (pilA) [Stutzerimonas decontaminans strain 19SMN4]
MKAQMQKGFTLIELMIVVAIIGILAAIAIPQYQNYIARSESNTGLQAVAALKTAVEDRLMRGETTFTLAELGTKEDASPL
GTIAADMDSDTGEGSLTFTFDGAASPKVKGKAHTLTRDGEGAWTCTSDVDAEFMPRGCEA

Nucleotide


Download         Length: 423 bp        

>NTDB_id=120132 UIB01_RS04670 WP_038657331.1 1019210..1019632(-) (pilA) [Stutzerimonas decontaminans strain 19SMN4]
ATGAAAGCTCAGATGCAGAAAGGTTTTACCCTGATCGAACTGATGATCGTCGTGGCCATCATCGGTATTCTCGCCGCGAT
CGCAATTCCGCAGTACCAGAACTACATCGCCCGCTCTGAATCCAACACTGGCTTGCAGGCTGTTGCAGCGCTGAAAACGG
CAGTCGAAGACCGTCTGATGCGCGGCGAAACAACTTTCACCCTAGCCGAACTGGGCACCAAGGAAGATGCAAGCCCGCTG
GGCACCATCGCTGCCGATATGGATAGCGATACCGGCGAAGGCTCGCTGACGTTCACCTTCGACGGCGCAGCCAGCCCGAA
GGTCAAAGGCAAGGCACACACCTTGACTCGTGATGGCGAAGGCGCCTGGACCTGCACCAGTGATGTGGACGCAGAGTTCA
TGCCGCGCGGTTGTGAAGCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-28)

Pilin (bacterial filament) (Pilin)

(37-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

56

100

0.6
  pilA Acinetobacter baumannii strain A118

51.825

97.857

0.507
  pilA Vibrio cholerae strain A1552

44

100

0.471
  pilA Vibrio cholerae C6706

44

100

0.471
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44

100

0.471
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.755

100

0.457
  pilA Vibrio parahaemolyticus RIMD 2210633

49.206

90

0.443
  pilA/pilA1 Eikenella corrodens VA1

35.802

100

0.414
  pilA2 Legionella pneumophila strain ERS1305867

41.007

99.286

0.407
  pilA2 Legionella pneumophila str. Paris

41.007

99.286

0.407
  pilA Haemophilus influenzae 86-028NP

38.462

100

0.393
  pilA Haemophilus influenzae Rd KW20

38.462

100

0.393
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.855

98.571

0.393
  comP Acinetobacter baylyi ADP1

35.57

100

0.379
  pilA Ralstonia pseudosolanacearum GMI1000

35.862

100

0.371

Multiple sequence alignment