Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   UIB01_RS04675 Genome accession   NZ_CP007509
Coordinates   1019954..1021666 (+) Length   570 a.a.
NCBI ID   WP_038657333.1    Uniprot ID   -
Organism   Stutzerimonas decontaminans strain 19SMN4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1014954..1026666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UIB01_RS04660 (UIB01_04725) cysD 1015747..1016664 (+) 918 WP_038657327.1 sulfate adenylyltransferase subunit CysD -
  UIB01_RS04665 (UIB01_04730) cysN 1016676..1018574 (+) 1899 WP_038657329.1 sulfate adenylyltransferase subunit CysN -
  UIB01_RS04670 (UIB01_04735) pilA 1019210..1019632 (-) 423 WP_038657331.1 pilin Machinery gene
  UIB01_RS04675 (UIB01_04740) pilB 1019954..1021666 (+) 1713 WP_038657333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  UIB01_RS04680 (UIB01_04745) pilC 1021669..1022889 (+) 1221 WP_038657335.1 type II secretion system F family protein Machinery gene
  UIB01_RS04685 (UIB01_04750) pilD 1022890..1023759 (+) 870 WP_038657337.1 A24 family peptidase Machinery gene
  UIB01_RS04690 (UIB01_04755) coaE 1023911..1024519 (+) 609 WP_038657340.1 dephospho-CoA kinase -
  UIB01_RS04695 (UIB01_04760) yacG 1024516..1024713 (+) 198 WP_038657341.1 DNA gyrase inhibitor YacG -
  UIB01_RS04700 (UIB01_04765) - 1024752..1025432 (-) 681 WP_038657343.1 energy-coupling factor ABC transporter permease -
  UIB01_RS04705 (UIB01_04770) - 1025499..1026536 (-) 1038 WP_038657345.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62343.30 Da        Isoelectric Point: 5.4768

>NTDB_id=120133 UIB01_RS04675 WP_038657333.1 1019954..1021666(+) (pilB) [Stutzerimonas decontaminans strain 19SMN4]
MSESISLSGLARQMVLAGVLDEKTAQQAQVQALRNKSSLVTYVVQSKLAKGRVVAELASEQFGVALLDLSALDKESLPKD
VVSEKLARQHRVLPLARRGNKLYLAVSDPANHQAVTDIQFSTGLTTEAILVEDDKLEQAIDKFFESTSSGLGDMGDMGDI
DLDGVDVETVSDDARGDESGGEADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLSPRI
SARLKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALK
QPQGMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCGSCKREVDVPRDALLRE
GFPEQKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTS
LEEVNRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=120133 UIB01_RS04675 WP_038657333.1 1019954..1021666(+) (pilB) [Stutzerimonas decontaminans strain 19SMN4]
ATGAGCGAGAGCATTTCCCTTTCCGGTCTGGCCCGGCAGATGGTGCTGGCGGGAGTGTTGGACGAAAAGACGGCCCAGCA
GGCGCAGGTTCAGGCGCTGCGTAATAAGTCATCTCTGGTTACCTACGTAGTACAAAGCAAGTTGGCGAAAGGGCGCGTTG
TTGCCGAGCTTGCCTCCGAGCAATTTGGTGTCGCCTTGCTGGACCTGTCCGCGTTGGACAAGGAGTCTCTACCGAAGGAC
GTAGTTAGTGAAAAGCTGGCTCGCCAACACCGAGTTTTGCCCCTGGCAAGGCGCGGCAACAAGCTGTATCTGGCTGTTTC
AGACCCTGCGAACCATCAGGCCGTTACTGATATCCAGTTCAGTACTGGGCTCACAACCGAAGCCATCCTGGTTGAGGATG
ACAAGCTCGAACAGGCCATCGATAAGTTCTTCGAAAGCACCTCGTCCGGCCTCGGTGACATGGGGGACATGGGCGACATT
GATCTGGATGGAGTGGACGTTGAAACCGTTTCCGATGACGCGCGGGGCGATGAGTCGGGCGGGGAGGCTGACGATGCGCC
AGTGGTGCGTTTCGTTAACAAAATGCTGCTCGATGCGATCCGTGGCGGTTCCTCCGACTTGCATTTCGAGCCCTACGAGA
AGGCTTATCGCGTGAGACTGCGTACCGACGGCATTCTGCATGAGGTCGCGCGCCCCCCTATCCAGCTTTCCCCGCGCATT
TCCGCGCGCCTTAAGGTGATGGCTGGCTTGGATATCTCCGAACGGCGTAAGCCGCAGGATGGCCGGATCAAGATGAAGCT
CTCCAAGAGCAAGGCCATAGACTTCCGAGTCAACACACTGCCGACGCTGTGGGGCGAGAAGATTGTGATGCGGATTCTCG
ACCCGTCGAGCGCTCAAATGGGTATCGACGCGCTGGGGTATGAGGAGGATCAGAAAGAGCTGTACATGAATGCCTTGAAG
CAACCGCAGGGCATGATTCTGGTTACGGGGCCTACTGGTTCCGGCAAGACGGTATCTCTATACACTGGCTTGAATATCCT
CAATACGGTGGACGTGAATATTTCCACGGCTGAGGATCCTGTGGAAATCAATCTTGAAGGCATTAACCAAGTCAACGTGA
ATCCTAAGCAGGGCATGGATTTCTCTCAGGCCCTTCGTGCGTTTCTACGTCAGGATCCGGACATTATCATGGTGGGTGAG
ATTCGGGACTTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACTGGCCATATGGTGATGTCTACCCTACACACCAA
CAGCGCGGCAGAAACGCTGACTCGCCTGCGCAATATGGGTGTGCCTGCCTTCAACATTGCTACCTCGGTGAATTTGATCA
TTGCGCAGCGCTTGGCCCGCAAGCTTTGCGGCAGTTGCAAGAGGGAGGTCGACGTTCCTCGTGATGCGCTGCTGCGCGAA
GGCTTTCCGGAGCAGAAGATCGGCACCTTCAAAATTTACGGCCCGGTTGGCTGCGATAACTGCAAGGGCGGCTACAAAGG
GCGTGTCGGTATTTATGAAGTGGTTAAAAACACGCCCTCGCTGCAGCGGATTATCATGGAGGACGGTAATTCAATCGACA
TTTCCACCCAGATGCGCAAAGACGGCTTCAACGACCTGCGGACATCGGCGCTGGTAAAGGCCATGCAGGGCGTAACAAGC
CTCGAAGAAGTCAACCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.86

100

0.602
  pilB Acinetobacter baylyi ADP1

59.507

99.649

0.593
  pilB Legionella pneumophila strain ERS1305867

55.672

100

0.56
  pilB Vibrio campbellii strain DS40M4

48.955

100

0.493
  pilB Vibrio cholerae strain A1552

53.981

90.351

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

52.174

92.807

0.484
  pilF Neisseria gonorrhoeae MS11

51.44

91.404

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

36.554

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

93.158

0.365
  pilF Thermus thermophilus HB27

36.59

98.772

0.361

Multiple sequence alignment