Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EZ55_RS15140 Genome accession   NZ_OX359235
Coordinates   3503194..3504237 (-) Length   347 a.a.
NCBI ID   WP_156078754.1    Uniprot ID   -
Organism   Alteromonas macleodii isolate Alteromonas macleodii BGP6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3498194..3509237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZ55_RS15110 (ALTBGP6_03056) - 3498629..3499357 (+) 729 WP_014980069.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EZ55_RS15115 (ALTBGP6_03057) gshB 3499496..3500449 (+) 954 WP_156078753.1 glutathione synthase -
  EZ55_RS15120 (ALTBGP6_03058) - 3500578..3501144 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  EZ55_RS15125 (ALTBGP6_03059) ruvX 3501137..3501568 (+) 432 WP_014980071.1 Holliday junction resolvase RuvX -
  EZ55_RS15130 (ALTBGP6_03060) - 3501615..3501932 (-) 318 WP_049586039.1 DUF2007 domain-containing protein -
  EZ55_RS15135 (ALTBGP6_03061) pilU 3502073..3503185 (-) 1113 WP_014977241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EZ55_RS15140 (ALTBGP6_03062) pilT 3503194..3504237 (-) 1044 WP_156078754.1 type IV pilus twitching motility protein PilT Machinery gene
  EZ55_RS15145 (ALTBGP6_03063) - 3504265..3504951 (+) 687 WP_039227916.1 YggS family pyridoxal phosphate-dependent enzyme -
  EZ55_RS15150 (ALTBGP6_03064) proC 3505022..3505843 (+) 822 WP_014950466.1 pyrroline-5-carboxylate reductase -
  EZ55_RS15155 (ALTBGP6_03065) - 3505864..3506400 (+) 537 WP_014950467.1 YggT family protein -
  EZ55_RS15160 (ALTBGP6_03066) - 3506497..3506970 (+) 474 WP_156078755.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38428.13 Da        Isoelectric Point: 6.5677

>NTDB_id=1155717 EZ55_RS15140 WP_156078754.1 3503194..3504237(-) (pilT) [Alteromonas macleodii isolate Alteromonas macleodii BGP6]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHGLVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITKQDAAAKSFDKQSMNSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1155717 EZ55_RS15140 WP_156078754.1 3503194..3504237(-) (pilT) [Alteromonas macleodii isolate Alteromonas macleodii BGP6]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCGGGCCTACCACCAAT
AATTCGTGTAGATGGCGAAATGCGTAAACTGAACGTGCCAGCCCTCGATCACAAGCAAGTACATGGTCTCGTTTATGAGA
TTATGAATGACATGCAGCGCAAAGAATACGAAGAAAACCTGGAAACCGATTTTTCTTTCGAAGTTAATGGCTTGTCGCGC
TTTCGTGTTAACGCGTTTGTGCAAAACCGAGGCGCCGCGGCAGTATTACGTACTATCCCCAGTAAAGTATTAACCTTAGA
CGACCTTGGCGCGCCTGAAATTTTCAAGGAAATTATAAATCAGCCTACCGGCATTGTACTTGTGACAGGTGCCACCGGCT
CGGGTAAAAGTACCACGCTTGCGGCCATGGTCGACCATATCAACACCCACAAGCGTGAACATATTCTTACCATTGAAGAC
CCTATCGAATTCGTGCACGACAACAAGCTTAGCTTGGTAAACCAGCGGGAAGTACACCGCGATACGCACTCGTTTAATAA
CGCACTGCGCTCGGCGCTTCGTGAAGACCCAGACGTAATTCTAGTCGGTGAGCTACGTGACTTAGAAACCATACGCCTTG
CCATTTCAGCTGCCGAAACCGGCCATTTAGTATTCGGCACTCTTCACACCAACTCTGCACCAAAAACCATTGACCGTATT
ATCGATGTTTTCCCGGCAGAAGAAAAAGCCATGGTGCGTTCTATGTTGTCGGAATCATTGCGTGCGGTTATTTCACAGAC
ACTGCTTAAGAAAGTAGGCGGGGGCCGAGTTGCGGCTCATGAAATCATGGTGGGGATCCCCGCTATTCGAAACCTTATTC
GAGAAGATAAAGTGCCGCAAATGTACTCGGTTATTCAAACGGGTCAGGCTCACGGTATGCAAACTATGGATCAGTGTCTT
CAGCGCTTAGTGGCGATGGGGGTTATCACCAAACAAGACGCCGCAGCAAAGTCATTTGATAAACAGTCGATGAACAGTTT
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.581

97.695

0.758
  pilT Acinetobacter baumannii strain A118

77.581

97.695

0.758
  pilT Acinetobacter nosocomialis M2

77.286

97.695

0.755
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Pseudomonas aeruginosa PAK

74.852

97.406

0.729
  pilT Legionella pneumophila strain Lp02

71.848

98.271

0.706
  pilT Legionella pneumophila strain ERS1305867

71.848

98.271

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria meningitidis 8013

65.029

99.712

0.648
  pilT Neisseria gonorrhoeae MS11

64.74

99.712

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

98.847

0.513
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

41.42

97.406

0.403

Multiple sequence alignment