Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EZ55_RS15135 Genome accession   NZ_OX359235
Coordinates   3502073..3503185 (-) Length   370 a.a.
NCBI ID   WP_014977241.1    Uniprot ID   A0AB33A1W2
Organism   Alteromonas macleodii isolate Alteromonas macleodii BGP6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3497073..3508185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZ55_RS15110 (ALTBGP6_03056) - 3498629..3499357 (+) 729 WP_014980069.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EZ55_RS15115 (ALTBGP6_03057) gshB 3499496..3500449 (+) 954 WP_156078753.1 glutathione synthase -
  EZ55_RS15120 (ALTBGP6_03058) - 3500578..3501144 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  EZ55_RS15125 (ALTBGP6_03059) ruvX 3501137..3501568 (+) 432 WP_014980071.1 Holliday junction resolvase RuvX -
  EZ55_RS15130 (ALTBGP6_03060) - 3501615..3501932 (-) 318 WP_049586039.1 DUF2007 domain-containing protein -
  EZ55_RS15135 (ALTBGP6_03061) pilU 3502073..3503185 (-) 1113 WP_014977241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EZ55_RS15140 (ALTBGP6_03062) pilT 3503194..3504237 (-) 1044 WP_156078754.1 type IV pilus twitching motility protein PilT Machinery gene
  EZ55_RS15145 (ALTBGP6_03063) - 3504265..3504951 (+) 687 WP_039227916.1 YggS family pyridoxal phosphate-dependent enzyme -
  EZ55_RS15150 (ALTBGP6_03064) proC 3505022..3505843 (+) 822 WP_014950466.1 pyrroline-5-carboxylate reductase -
  EZ55_RS15155 (ALTBGP6_03065) - 3505864..3506400 (+) 537 WP_014950467.1 YggT family protein -
  EZ55_RS15160 (ALTBGP6_03066) - 3506497..3506970 (+) 474 WP_156078755.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40781.69 Da        Isoelectric Point: 6.0487

>NTDB_id=1155716 EZ55_RS15135 WP_014977241.1 3502073..3503185(-) (pilU) [Alteromonas macleodii isolate Alteromonas macleodii BGP6]
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTESATSEEASLALVHEAMTEKQQNEFHTTKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1155716 EZ55_RS15135 WP_014977241.1 3502073..3503185(-) (pilU) [Alteromonas macleodii isolate Alteromonas macleodii BGP6]
ATGTTAGATTCACTACTACAAAAAATGGTCGATACCAACGCATCGGATTTATTCGTAACGGCGGGGTTTCCGGTAAGTGC
CAAAATTAACGGCAAACTTACGCCAGTGACGGAAAGCGCGACCTCAGAAGAGGCATCGCTGGCGCTAGTTCACGAAGCGA
TGACTGAAAAACAGCAGAATGAGTTTCACACGACCAAAGAATGTAATTTCGCCATAGTACGAGAGGGCATTGGTCGTTTC
CGCTGCTCTGCATTTTGGCAGCGTGACCAAGCGGGCATGGTGATTCGCCGCATCGTTACTCAAATTCCGAAAGCTGATGA
TTTGGGTCTGCCGCGAGTATTAAAAGAAATTATTATGGCTAAACGCGGTCTTGTTCTATTTGTAGGTGGAACAGGCACGG
GTAAATCAACGTCATTGGCTGCGCTTATTGGGCATAGAAATGAAAATTCGCATGGGCATATACTGACTATTGAAGACCCC
ATTGAATTTGTGCACGAGCATAAGAACTGCGTTATTACCCAGCGCGAGGTGGGCATAGATACTCAGTCTTTCGACGACGC
GCTTAAAAGTTCGCTACGTCAGGCGCCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAAACCATGGAATATGCTA
TGTCGTTTGCTGATACAGGGCATTTGTGTGTGGCTACGCTGCACGCTAACAACGCGAACCAAGCTATTGAACGTATTATG
CATTTAGCGCCGAAAGATCAGCACGATAAGTTGCGCTTTGACTTAAGCTTAAACATCCGCGCCATTGTTGCGCAGCAACT
AGTACCTACGCCAGACGGCAAGGGTAGGGTTGCGGCAATAGAAATACTGCTTAATTCGCCGCTAGTGCGCGACCTAATAC
AGCGCAATGAAATAGGCGCGTTAAAAGAAGCAATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGCTTTGATATGGCGCTG
TATGATTTGTACAAAGCGGGTAAGATTGATATGGACCAAGCGCTGCACCACGCCGACTCGCCCAACGATTTGCGCTTGAT
GATCAAGCTTGATACCAGCGACGGTTCTAGCTTAGGTACACTGTCAAATGTATCTATTGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

59.143

94.595

0.559
  pilU Vibrio cholerae strain A1552

56.25

99.459

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

91.892

0.392
  pilT Acinetobacter nosocomialis M2

39.412

91.892

0.362
  pilT Acinetobacter baumannii D1279779

39.412

91.892

0.362
  pilT Acinetobacter baumannii strain A118

39.412

91.892

0.362

Multiple sequence alignment