Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGHAD_RS01585 Genome accession   NZ_AP031500
Coordinates   379050..380084 (+) Length   344 a.a.
NCBI ID   WP_382416747.1    Uniprot ID   -
Organism   Gilvimarinus japonicus strain 12-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 374050..385084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHAD_RS01565 (R50076_03200) - 374586..376574 (-) 1989 WP_382416741.1 dynamin family protein -
  ACGHAD_RS01570 (R50076_03210) - 376732..377322 (-) 591 WP_339617054.1 YggT family protein -
  ACGHAD_RS01575 (R50076_03220) proC 377419..378240 (-) 822 WP_382416743.1 pyrroline-5-carboxylate reductase -
  ACGHAD_RS01580 (R50076_03230) - 378257..378940 (-) 684 WP_382416745.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHAD_RS01585 (R50076_03240) pilT 379050..380084 (+) 1035 WP_382416747.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHAD_RS01590 (R50076_03250) pilU 380167..381312 (+) 1146 WP_339617058.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHAD_RS01595 (R50076_03260) ruvX 381382..381828 (-) 447 WP_382416749.1 Holliday junction resolvase RuvX -
  ACGHAD_RS01600 (R50076_03270) - 381821..382417 (-) 597 WP_382416751.1 YqgE/AlgH family protein -
  ACGHAD_RS01605 (R50076_03280) - 382479..383384 (-) 906 WP_382416752.1 energy transducer TonB -
  ACGHAD_RS01610 (R50076_03290) gshB 383387..384349 (-) 963 WP_339617062.1 glutathione synthase -
  ACGHAD_RS01615 (R50076_03300) pilG 384667..385062 (+) 396 WP_339617064.1 twitching motility response regulator PilG Regulator

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38312.96 Da        Isoelectric Point: 6.5054

>NTDB_id=109945 ACGHAD_RS01585 WP_382416747.1 379050..380084(+) (pilT) [Gilvimarinus japonicus strain 12-2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHSLIYEIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEELGMGQVFRDICAVPRGLVLVTGPTGSGKSTTLAAMMDYINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKSMVRSMLSESLQAVVSQTLLKKNGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGASVGMQTMDQCL
EDMVARRVINRDVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=109945 ACGHAD_RS01585 WP_382416747.1 379050..380084(+) (pilT) [Gilvimarinus japonicus strain 12-2]
ATGGATATCACGGAGCTTCTCGCCTTTAGCGCCAAGCAGGGCGCTTCGGATTTGCACCTCTCGGCGGGCCTGCCGCCAAT
GATTCGCGTCGACGGCGACGTGCGTCGAATCAACCTGCCGCCGATGGAGCACAAGCAGGTGCACAGTTTGATCTACGAGA
TCATGAATGACAAGCAACGCAAGGATTTTGAAGAGTTTTTAGAAACTGACTTCTCGTTTGAGGTACCTGGCGTTGCGCGC
TTTCGGGTTAATGCGTTTAACCAAAACCGCGGCTCGGGTGCGGTATTTCGGACCATTCCCTCAAAAGTTTTAACCATGGA
AGAGCTGGGTATGGGGCAGGTATTTCGCGATATTTGCGCGGTGCCCCGTGGCTTGGTGTTGGTTACCGGACCTACGGGTT
CGGGTAAATCCACCACCCTGGCAGCGATGATGGATTATATTAACGACAATAAATACGAACACATTCTGACCATTGAAGAC
CCTATCGAGTTTGTGCACGAGAGTAAAAAGTGTCTGGTTAACCAGCGCGAAGTGCACCGTGATACCCACGGCTTTTCTGA
GGCATTGCGCTCGGCCCTGCGGGAAGACCCGGACATTATTCTGGTGGGTGAGCTGCGGGATTTGGAAACCATTCGTTTGG
CGCTGACGGCGGCTGAGACCGGTCACTTAGTGTTTGGTACTTTGCACACCACCAGTGCGGCAAAAACCATTGACCGGGTG
GTGGACGTGTTTCCCGCAAACGAGAAGTCCATGGTGCGCTCGATGTTGTCGGAGTCGCTGCAAGCGGTGGTGTCGCAAAC
ACTACTCAAGAAAAACGGCGGCGGCCGGGTGGCGGCGCACGAAATTATGCGCGGCACCTCGGCGATCCGTAACTTGATTC
GCGAAGATAAAGTCGCCCAGATGTACTCGGCGATTCAGACCGGCGCTTCAGTGGGCATGCAGACGATGGATCAGTGCCTA
GAAGATATGGTGGCCCGTCGGGTGATTAACCGTGACGTCGCCCGTGAAAAGGCCAAAATGCCAGAGAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

88.663

100

0.887
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

40.923

94.477

0.387
  pilU Pseudomonas stutzeri DSM 10701

40.741

94.186

0.384
  pilU Acinetobacter baylyi ADP1

37.283

100

0.375

Multiple sequence alignment