Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGHAD_RS01590 Genome accession   NZ_AP031500
Coordinates   380167..381312 (+) Length   381 a.a.
NCBI ID   WP_339617058.1    Uniprot ID   -
Organism   Gilvimarinus japonicus strain 12-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375167..386312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHAD_RS01570 (R50076_03210) - 376732..377322 (-) 591 WP_339617054.1 YggT family protein -
  ACGHAD_RS01575 (R50076_03220) proC 377419..378240 (-) 822 WP_382416743.1 pyrroline-5-carboxylate reductase -
  ACGHAD_RS01580 (R50076_03230) - 378257..378940 (-) 684 WP_382416745.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHAD_RS01585 (R50076_03240) pilT 379050..380084 (+) 1035 WP_382416747.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHAD_RS01590 (R50076_03250) pilU 380167..381312 (+) 1146 WP_339617058.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHAD_RS01595 (R50076_03260) ruvX 381382..381828 (-) 447 WP_382416749.1 Holliday junction resolvase RuvX -
  ACGHAD_RS01600 (R50076_03270) - 381821..382417 (-) 597 WP_382416751.1 YqgE/AlgH family protein -
  ACGHAD_RS01605 (R50076_03280) - 382479..383384 (-) 906 WP_382416752.1 energy transducer TonB -
  ACGHAD_RS01610 (R50076_03290) gshB 383387..384349 (-) 963 WP_339617062.1 glutathione synthase -
  ACGHAD_RS01615 (R50076_03300) pilG 384667..385062 (+) 396 WP_339617064.1 twitching motility response regulator PilG Regulator
  ACGHAD_RS01620 (R50076_03310) pilH 385121..385483 (+) 363 WP_339617065.1 twitching motility response regulator PilH -
  ACGHAD_RS01625 (R50076_03320) - 385567..386112 (+) 546 WP_339617066.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42353.53 Da        Isoelectric Point: 6.1369

>NTDB_id=109946 ACGHAD_RS01590 WP_339617058.1 380167..381312(+) (pilU) [Gilvimarinus japonicus strain 12-2]
MDFDRLLQLMVDKGASDLFITAGVPPSIKLHGKVVPATATALTPEKVRELVLSVMNEKQRREFLDNKELNFAISARGIGR
FRASAFYQRNLAGMVLRRIETTIPRIDDLNLPDVIKDLAMTKRGLVMFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
PIEFIHQHEGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEVRSRETMEHAIAFAETGHLCLCTLHANNSNQALDRI
IHFFPADRHRQLWMDLSLNLRGIVAQQLIPTPDGQGRRACLEVLLNTPLASDMIRKGEVSDLKELMKKSTELGMQTFDQA
LYALYDNGEITYEDALSHADSPNDLRLMIKLGSETDAAYLSNAADGLSIEEEDTGRGGRMF

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=109946 ACGHAD_RS01590 WP_339617058.1 380167..381312(+) (pilU) [Gilvimarinus japonicus strain 12-2]
ATGGATTTCGATCGATTACTGCAGCTGATGGTAGATAAAGGTGCATCGGATTTATTTATTACCGCCGGTGTTCCCCCTTC
CATTAAATTGCATGGCAAGGTGGTGCCGGCCACCGCGACGGCGTTAACGCCGGAAAAAGTGCGCGAGCTGGTGTTGTCGG
TGATGAACGAAAAACAACGCCGCGAGTTTTTGGATAATAAAGAGCTCAACTTTGCCATTAGTGCACGGGGTATTGGCCGG
TTTCGGGCCAGTGCATTTTATCAGCGCAACTTGGCGGGCATGGTGCTGCGGCGTATCGAAACCACCATTCCCCGTATTGA
CGATTTGAACTTGCCAGATGTGATTAAAGATTTGGCGATGACCAAACGTGGTTTGGTCATGTTTGTGGGGGCAACGGGTA
CCGGTAAATCTACCTCGCTCGCCTCGATGATTGGCCACCGCAACCGCAATTCGAAAGGGCATATTATTTCCATCGAAGAC
CCAATTGAATTTATCCACCAGCACGAGGGCTGCATTATTACCCAGCGCGAGGTGGGTATTGATACCGACTCGTTTGAGAT
TGCGCTAAAAAATACGTTGCGTCAGGCGCCCGATGTTATTTTGATTGGTGAGGTTCGTTCGCGCGAAACGATGGAGCACG
CCATCGCTTTTGCCGAGACTGGCCACTTGTGTCTGTGTACGCTGCACGCTAACAACTCTAACCAAGCGCTGGATCGGATT
ATTCACTTCTTTCCCGCCGATCGTCACCGCCAGTTGTGGATGGACTTGTCGCTTAACTTAAGAGGCATTGTGGCACAGCA
GTTGATACCCACCCCCGACGGTCAAGGTCGGCGCGCCTGTTTGGAGGTGTTGCTGAATACGCCTCTGGCGTCTGACATGA
TTCGTAAAGGCGAGGTGAGCGACCTTAAAGAGCTGATGAAAAAATCCACTGAGCTGGGGATGCAGACGTTCGATCAGGCG
CTGTACGCGCTCTATGATAATGGTGAAATTACCTACGAAGACGCTTTGTCTCACGCCGACTCGCCTAACGATTTGCGCTT
GATGATTAAGCTTGGCTCCGAGACCGATGCGGCTTATCTATCGAACGCGGCCGATGGCTTGTCGATCGAGGAAGAGGACA
CGGGTCGCGGCGGACGGATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.64

100

0.766
  pilU Acinetobacter baylyi ADP1

67.227

93.701

0.63
  pilU Vibrio cholerae strain A1552

55.143

91.864

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.559

100

0.428

Multiple sequence alignment