Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACGHTT_RS17920 Genome accession   NZ_AP031497
Coordinates   4049297..4050331 (-) Length   344 a.a.
NCBI ID   WP_290259710.1    Uniprot ID   -
Organism   Simiduia litorea strain KMM 9504     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4044297..4055331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHTT_RS17890 (R50072_35750) pilG 4044410..4044805 (-) 396 WP_183907265.1 twitching motility response regulator PilG Regulator
  ACGHTT_RS17895 (R50072_35770) gshB 4045131..4046096 (+) 966 WP_390592353.1 glutathione synthase -
  ACGHTT_RS17900 (R50072_35780) - 4046101..4047012 (+) 912 WP_390592354.1 energy transducer TonB -
  ACGHTT_RS17905 (R50072_35790) - 4047051..4047644 (+) 594 WP_390592355.1 YqgE/AlgH family protein -
  ACGHTT_RS17910 (R50072_35800) ruvX 4047641..4048066 (+) 426 WP_390592356.1 Holliday junction resolvase RuvX -
  ACGHTT_RS17915 (R50072_35810) pilU 4048142..4049284 (-) 1143 WP_390592357.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHTT_RS17920 (R50072_35820) pilT 4049297..4050331 (-) 1035 WP_290259710.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHTT_RS17925 (R50072_35830) - 4050447..4051142 (+) 696 WP_390592359.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHTT_RS17930 (R50072_35840) proC 4051202..4052017 (+) 816 WP_390592360.1 pyrroline-5-carboxylate reductase -
  ACGHTT_RS17935 (R50072_35850) - 4052019..4052609 (+) 591 WP_390592361.1 YggT family protein -
  ACGHTT_RS17940 (R50072_35860) - 4052636..4053604 (-) 969 WP_390592362.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38219.00 Da        Isoelectric Point: 7.0158

>NTDB_id=109913 ACGHTT_RS17920 WP_290259710.1 4049297..4050331(-) (pilT) [Simiduia litorea strain KMM 9504]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPPMQHKQVHGLIYEIMNDRQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLNMEELGMGQVFRDICNTPRGICLVTGPTGSGKSTTLAAMVDYINDNKYQHILTIED
PIEFVHESKKCLINQREVHRDTHGFSAALRSALREDPDIILVGEMRDLETIKLALEAAETGHLVFGTLHTQSAAKTIDRI
IDVFPAAEKAMVRSMLSESLQAVISQTLMKKTGGGRVAAHEIMRGTPAIRNLIREDKVAQMYSAIQTGAAVGMQTMDQCL
ADMVARRIIGRDVAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=109913 ACGHTT_RS17920 WP_290259710.1 4049297..4050331(-) (pilT) [Simiduia litorea strain KMM 9504]
ATGGATATTACCGAACTTTTGGCATTTAGCGCTAAGCAGGGCGCATCAGATTTACACCTTTCGTCTGGTTTGCCGCCCAT
GATACGCGTTGATGGTGATGTGCGCCGTATTAATTTGCCGCCCATGCAGCACAAGCAAGTGCACGGCCTTATTTATGAAA
TCATGAACGACAGGCAGCGCAAAGACTACGAAGAATTTTTAGAAACGGATTTCTCGTTCGAAGTACCTGGTGTCGCGCGC
TTCCGGGTTAACGCCTTCAACCAAAACCGAGGCGCTGGCGCGGTATTTCGTACCATTCCGTCAAAAGTTTTGAACATGGA
AGAGTTGGGTATGGGGCAGGTGTTTCGCGATATCTGTAACACGCCGCGGGGTATTTGCCTGGTGACGGGGCCCACCGGTT
CCGGTAAGTCCACCACCCTTGCGGCCATGGTCGATTATATTAACGACAACAAATACCAGCACATTCTCACCATCGAAGAC
CCAATCGAATTCGTCCACGAAAGTAAAAAGTGTTTGATTAACCAGCGCGAAGTGCATCGCGATACGCACGGTTTTTCCGC
CGCACTGCGCTCCGCTTTGCGTGAAGACCCGGATATTATTCTGGTGGGTGAGATGCGCGACTTGGAAACCATTAAATTGG
CGCTTGAAGCAGCGGAAACCGGTCACTTGGTCTTCGGCACTTTGCACACCCAGTCGGCGGCAAAAACCATTGACCGAATT
ATCGACGTATTTCCCGCCGCTGAAAAAGCCATGGTGCGCTCGATGTTATCGGAATCTTTGCAGGCAGTTATTTCGCAAAC
CTTGATGAAAAAAACCGGCGGTGGCCGCGTGGCTGCCCATGAAATTATGCGGGGTACTCCGGCTATTCGAAACTTGATTC
GCGAAGATAAAGTGGCGCAAATGTATTCCGCCATTCAGACCGGCGCCGCCGTGGGTATGCAGACGATGGATCAGTGTTTG
GCTGACATGGTAGCCAGGCGCATTATTGGTCGGGATGTGGCGCGCGAGAAAGCTAAAATTCCGGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

85.756

100

0.858
  pilT Pseudomonas stutzeri DSM 10701

85.465

100

0.855
  pilT Acinetobacter baumannii D1279779

81.977

100

0.82
  pilT Acinetobacter baumannii strain A118

81.977

100

0.82
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.517

95.64

0.674
  pilT Vibrio cholerae strain A1552

70.517

95.64

0.674
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384
  pilU Pseudomonas stutzeri DSM 10701

40.123

94.186

0.378
  pilU Vibrio cholerae strain A1552

39.385

94.477

0.372

Multiple sequence alignment