Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGHTT_RS17915 Genome accession   NZ_AP031497
Coordinates   4048142..4049284 (-) Length   380 a.a.
NCBI ID   WP_390592357.1    Uniprot ID   -
Organism   Simiduia litorea strain KMM 9504     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4043142..4054284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHTT_RS17880 (R50072_35730) - 4043401..4043955 (-) 555 WP_290259696.1 chemotaxis protein CheW -
  ACGHTT_RS17885 (R50072_35740) pilH 4043986..4044348 (-) 363 WP_290259697.1 twitching motility response regulator PilH -
  ACGHTT_RS17890 (R50072_35750) pilG 4044410..4044805 (-) 396 WP_183907265.1 twitching motility response regulator PilG Regulator
  ACGHTT_RS17895 (R50072_35770) gshB 4045131..4046096 (+) 966 WP_390592353.1 glutathione synthase -
  ACGHTT_RS17900 (R50072_35780) - 4046101..4047012 (+) 912 WP_390592354.1 energy transducer TonB -
  ACGHTT_RS17905 (R50072_35790) - 4047051..4047644 (+) 594 WP_390592355.1 YqgE/AlgH family protein -
  ACGHTT_RS17910 (R50072_35800) ruvX 4047641..4048066 (+) 426 WP_390592356.1 Holliday junction resolvase RuvX -
  ACGHTT_RS17915 (R50072_35810) pilU 4048142..4049284 (-) 1143 WP_390592357.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHTT_RS17920 (R50072_35820) pilT 4049297..4050331 (-) 1035 WP_290259710.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGHTT_RS17925 (R50072_35830) - 4050447..4051142 (+) 696 WP_390592359.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGHTT_RS17930 (R50072_35840) proC 4051202..4052017 (+) 816 WP_390592360.1 pyrroline-5-carboxylate reductase -
  ACGHTT_RS17935 (R50072_35850) - 4052019..4052609 (+) 591 WP_390592361.1 YggT family protein -
  ACGHTT_RS17940 (R50072_35860) - 4052636..4053604 (-) 969 WP_390592362.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42395.76 Da        Isoelectric Point: 6.1375

>NTDB_id=109912 ACGHTT_RS17915 WP_390592357.1 4048142..4049284(-) (pilU) [Simiduia litorea strain KMM 9504]
MDFDRLLSLMVEKGASDLFITAGVPPSIKLHGKVVPVTTTPLSPEKSREVVLSVMNEKQRVEFIEAKELNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPKIDDLGLPEIIKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNRNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTDSFEIALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHSQLWMDLALNLRGIIAQQLIPTPDGLGRRACLEVLLNTPLAQDLIRKGSVHELKPLMKKSTELGMQTFDQA
LYDLYDRGEITYEDALSYADSPNDLRLLIKLGSETDAEYLSNAADELTIEKEEPNNGRMY

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=109912 ACGHTT_RS17915 WP_390592357.1 4048142..4049284(-) (pilU) [Simiduia litorea strain KMM 9504]
ATGGATTTTGATCGATTATTAAGCCTGATGGTTGAGAAGGGCGCCTCGGATTTGTTTATCACGGCGGGCGTTCCACCGTC
GATTAAATTGCACGGTAAAGTTGTGCCAGTCACCACCACGCCTTTGTCGCCAGAGAAATCGCGAGAAGTGGTGTTGAGCG
TGATGAACGAAAAACAACGGGTGGAATTTATTGAGGCCAAAGAATTAAACTTTGCGATTTCTGCGCGTGGTATCGGTCGT
TTTCGTGTTAGTGCGTTTTATCAACGCAACTTGGCCGGCATGGTGTTGCGTCGCATTGAAACCAATATTCCAAAAATTGA
CGACTTGGGCTTGCCGGAAATTATCAAAGAGCTGGCGATGACCAAGCGCGGCTTGATCATTTTTGTGGGCGCCACCGGTA
CGGGTAAGTCGACTTCGCTGGCGTCTATGATTGGCCACCGAAATCGAAACTCCAAAGGTCACATCATTACCATTGAAGAC
CCGATTGAATTTATTCATCAGCATCAGGGTTGTATTGTGACCCAGCGCGAAGTGGGTATTGATACCGATAGCTTTGAGAT
AGCCCTGAAAAATACCTTGCGTCAGGCGCCCGATGTTATCTTGATTGGCGAAGTGCGCTCGCGCGAGACCATGGATCACG
CTATCGCCTTTGCCGAAACCGGCCATCTGTGCTTATGTACCTTGCACGCCAACAACGCCAACCAGGCACTCGATCGTATT
ATTCACTTCTTCCCAGCCGATCGTCACTCGCAGTTGTGGATGGATTTGGCGCTAAACTTGCGTGGCATTATCGCCCAGCA
ATTAATTCCAACGCCGGATGGGCTGGGGCGCCGCGCCTGTTTGGAGGTGTTGCTCAATACGCCTTTGGCGCAGGATTTAA
TTCGCAAGGGTTCGGTTCACGAACTGAAGCCGCTGATGAAAAAGTCGACCGAGCTGGGAATGCAAACCTTCGATCAGGCG
CTCTACGATTTGTACGACCGCGGCGAAATTACTTACGAAGACGCGCTGTCTTACGCTGACTCACCTAACGATCTGCGCCT
GTTGATTAAACTGGGATCGGAAACCGATGCGGAGTACCTCAGTAATGCTGCCGATGAGTTGACCATCGAGAAAGAGGAGC
CAAATAACGGCAGAATGTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(35-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.368

100

0.774
  pilU Acinetobacter baylyi ADP1

65.354

100

0.655
  pilU Vibrio cholerae strain A1552

53.226

97.895

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.54

91.579

0.408
  pilT Pseudomonas aeruginosa PAK

40

90.789

0.363
  pilT Legionella pneumophila strain Lp02

41.194

88.158

0.363
  pilT Legionella pneumophila strain ERS1305867

41.194

88.158

0.363

Multiple sequence alignment