Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYR_RS01340 Genome accession   NZ_CP180556
Coordinates   317015..318049 (+) Length   344 a.a.
NCBI ID   WP_415902622.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD29_5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 312015..323049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYR_RS01310 (ACMXYR_01310) - 312208..312657 (-) 450 WP_415902617.1 DUF4426 domain-containing protein -
  ACMXYR_RS01315 (ACMXYR_01315) metW 312670..313260 (-) 591 WP_415897395.1 methionine biosynthesis protein MetW -
  ACMXYR_RS01320 (ACMXYR_01320) metX 313260..314429 (-) 1170 WP_415902618.1 homoserine O-succinyltransferase MetX -
  ACMXYR_RS01325 (ACMXYR_01325) - 314727..315254 (-) 528 WP_415902619.1 YggT family protein -
  ACMXYR_RS01330 (ACMXYR_01330) proC 315382..316209 (-) 828 WP_415902620.1 pyrroline-5-carboxylate reductase -
  ACMXYR_RS01335 (ACMXYR_01335) - 316228..316923 (-) 696 WP_415902621.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYR_RS01340 (ACMXYR_01340) pilT 317015..318049 (+) 1035 WP_415902622.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYR_RS01345 (ACMXYR_01345) pilU 318072..319238 (+) 1167 WP_415886689.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYR_RS01350 (ACMXYR_01350) - 319300..320574 (-) 1275 WP_415902623.1 dihydroorotase -
  ACMXYR_RS01355 (ACMXYR_01355) - 320580..321584 (-) 1005 WP_415886692.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYR_RS01360 (ACMXYR_01360) pyrR 321594..322109 (-) 516 WP_415886693.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYR_RS01365 (ACMXYR_01365) ruvX 322090..322566 (-) 477 WP_415902624.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38277.99 Da        Isoelectric Point: 6.6681

>NTDB_id=1096553 ACMXYR_RS01340 WP_415902622.1 317015..318049(+) (pilT) [Neptuniibacter sp. QD29_5]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLIYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFKDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGASFGMKTLDQSL
QELMQKGLITREIARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096553 ACMXYR_RS01340 WP_415902622.1 317015..318049(+) (pilT) [Neptuniibacter sp. QD29_5]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGTGCATCTGACTTGCATATATCTGCAGGGATGCCTCCAAT
CATTCGTGTTGACGGTGATGTACGTCGCATAAAACTGCCTTCGCTGGACCACAAGCAGGTCCATACACTCATATATGACA
TTATGAACGATAAACAACGTAAAGAGTACGAAGATGTACTCGAAACGGATTTTTCGTTCGAAGTCCCTGGTCTAGCGCGT
TTTCGTGTAAACGCATTTAACCAAAATCGCGGAGCGGGTGCCGTTTTCCGTACGATCCCCAGTAAAGTTTTGACCTTAGA
TGATCTGGATATGGGCAAGGTTTTCAAGGATCTCTCAGAAAAACCACGTGGTTTAGTACTGGTCACGGGGCCAACGGGTT
CCGGTAAAAGTACCACATTGGCTGCGATGATCGATTACATTAATGATACTCGTGCTGATCATATTTTGACTATCGAAGAC
CCAATCGAATTCGTGCATGAAAGCAAGAAATGCTTGGTAAACCAGCGTGAGGTACATAGGGATACTCATAGTTTTTCTAA
TGCTCTTCGTTCGGCGTTGCGTGAAGACCCGGACATTATCCTGGTGGGCGAGATGCGAGACTTGGAAACTATCCGTCTTG
CTTTGACTGCAGCAGAAACGGGACACTTAGTATTTGGTACATTGCATACAACCTCTGCAGCAAAAACAATTGACCGTGTT
ATTGACGTATTTCCAGCCGCTGAGAAGGATATGGTGAGATCCATGCTTTCAGAGTCGCTGATGGGTGTTATATCGCAAAC
TCTTTTGAAAAAGCCAAAAGGTGGACGTGTTGCTGCTCATGAAATTATGATCGGTACGCCGGCTATTCGTAACCTTATCC
GAGAGGATAAAGTAGCCCAGATGTACTCTGCTATCCAGACGGGTGCATCATTTGGCATGAAAACTTTGGATCAGTCTCTA
CAAGAGTTGATGCAAAAAGGATTGATTACTCGCGAAATTGCGCGTGAACGAGCAGCGAACCCTCAACAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.14

100

0.831
  pilT Pseudomonas stutzeri DSM 10701

81.977

100

0.82
  pilT Acinetobacter nosocomialis M2

80.523

100

0.805
  pilT Acinetobacter baumannii D1279779

80.523

100

0.805
  pilT Acinetobacter baumannii strain A118

80.523

100

0.805
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Neisseria meningitidis 8013

66.764

99.709

0.666
  pilT Neisseria gonorrhoeae MS11

66.472

99.709

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.997

95.64

0.66
  pilT Vibrio cholerae strain A1552

68.997

95.64

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Vibrio cholerae strain A1552

41.538

94.477

0.392
  pilB Acinetobacter baylyi ADP1

32.292

100

0.36

Multiple sequence alignment