Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYR_RS01345 Genome accession   NZ_CP180556
Coordinates   318072..319238 (+) Length   388 a.a.
NCBI ID   WP_415886689.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD29_5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 313072..324238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYR_RS01320 (ACMXYR_01320) metX 313260..314429 (-) 1170 WP_415902618.1 homoserine O-succinyltransferase MetX -
  ACMXYR_RS01325 (ACMXYR_01325) - 314727..315254 (-) 528 WP_415902619.1 YggT family protein -
  ACMXYR_RS01330 (ACMXYR_01330) proC 315382..316209 (-) 828 WP_415902620.1 pyrroline-5-carboxylate reductase -
  ACMXYR_RS01335 (ACMXYR_01335) - 316228..316923 (-) 696 WP_415902621.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYR_RS01340 (ACMXYR_01340) pilT 317015..318049 (+) 1035 WP_415902622.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYR_RS01345 (ACMXYR_01345) pilU 318072..319238 (+) 1167 WP_415886689.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYR_RS01350 (ACMXYR_01350) - 319300..320574 (-) 1275 WP_415902623.1 dihydroorotase -
  ACMXYR_RS01355 (ACMXYR_01355) - 320580..321584 (-) 1005 WP_415886692.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYR_RS01360 (ACMXYR_01360) pyrR 321594..322109 (-) 516 WP_415886693.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYR_RS01365 (ACMXYR_01365) ruvX 322090..322566 (-) 477 WP_415902624.1 Holliday junction resolvase RuvX -
  ACMXYR_RS01370 (ACMXYR_01370) - 322620..323192 (-) 573 WP_415902625.1 YqgE/AlgH family protein -
  ACMXYR_RS01375 (ACMXYR_01375) - 323230..324225 (-) 996 WP_415902626.1 energy transducer TonB -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43397.45 Da        Isoelectric Point: 6.0150

>NTDB_id=1096554 ACMXYR_RS01345 WP_415886689.1 318072..319238(+) (pilU) [Neptuniibacter sp. QD29_5]
MDFTQLLKVMVDRDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGCHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYVHEHKQSIITQREVGIDTDSFDVALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKDVVKKSTNLGMQTFDQS
LFNLYKDGVITYDVALAHADSPNDLRLMIKLSADTNPELAPDEDNQSFHLQDEEDYLHNEGDANVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1096554 ACMXYR_RS01345 WP_415886689.1 318072..319238(+) (pilU) [Neptuniibacter sp. QD29_5]
GTGGATTTTACGCAACTACTAAAGGTGATGGTTGATAGGGATGCCTCTGACCTTTTTGTAACGGCCGGTGCAAGGCCGAC
GATTAAAGTTGATGGTACTTTGAAACCGTTAACAAAAGACACCCTTAAGCCATCACAGGCTCGAGCTCTTGTGTACAGTA
CTATGAATGACAAGCAGCTTGCGGAGTTTGAGGGTTGTCATGAGTGTAACTTTGCGATTAGTGCGCCAGGTTTGGGACGT
TTTCGTGTATCAGCATTTTTCCAACGTAATTCAGCGGGTATGGTATTAAGGAAGATTAATAACTATATACCAAGCTTAGA
AGAGTTGAATCTGCCACCAATCCTACGTGATTTGTCTATGACAAAACGAGGATTAATCTTATTTGTTGGTGGTACCTCTA
CAGGTAAATCAACCTCTTTAGCATCGATGATTGATTACCGTAATACCAATCATCGGGGTCATATTATTACCATCGAAGAT
CCGATTGAGTATGTTCACGAACATAAGCAAAGTATCATCACTCAACGTGAAGTAGGTATCGATACCGACTCGTTTGATGT
AGCACTAAAAAATACGCTCCGTCAGGCACCAGATGTAATTTTGATGGGTGAGATCCGTACTAGGGAGACGATGCAGCACG
GTATCGTATTTGCTGAAACAGGTCATTTAACTCTAGCAACTTTACACGCTAATAATGCCAACCAAGCATTGGACCGTGTA
ATTAGCTTCTTCCCGCCTGAGCATCATGATCAGTTGTGGATGGATCTTTCATTAAACCTTAAAGCGATTATTGCGCAACA
ACTGTTACCTACTAAAGATGGTAAGGGACGTCGTGCAGCGATTGAAGTATTGATCAACACGCCATTAATTCAGGATCTGA
TTCGTAAAGGTGAAGTTCATGAAATTAAAGATGTGGTTAAGAAATCGACCAACTTAGGTATGCAGACGTTCGACCAATCT
TTGTTTAATCTCTACAAAGATGGGGTGATTACTTACGATGTAGCACTAGCCCATGCTGATTCGCCAAACGATCTTCGTCT
GATGATTAAGTTGAGTGCTGATACGAATCCTGAATTGGCTCCAGATGAAGATAATCAATCTTTCCATCTACAGGATGAAG
AGGATTATTTGCATAACGAAGGTGATGCTAACGTTAAGGGTATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.877

92.268

0.626
  pilU Acinetobacter baylyi ADP1

60.833

92.784

0.564
  pilU Vibrio cholerae strain A1552

55.143

90.206

0.497
  pilT Acinetobacter baylyi ADP1

44.737

88.144

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

88.918

0.392
  pilT Acinetobacter nosocomialis M2

43.86

88.144

0.387
  pilT Acinetobacter baumannii D1279779

43.86

88.144

0.387
  pilT Acinetobacter baumannii strain A118

43.86

88.144

0.387
  pilT Pseudomonas stutzeri DSM 10701

42.609

88.918

0.379
  pilT Pseudomonas aeruginosa PAK

42.609

88.918

0.379

Multiple sequence alignment