Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACETK7_RS21220 Genome accession   NZ_CP169287
Coordinates   4564442..4564852 (+) Length   136 a.a.
NCBI ID   WP_214378785.1    Uniprot ID   -
Organism   Pseudomonas hormoni strain G20-18_CNT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4559442..4569852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACETK7_RS21195 (ACETK7_21195) yacG 4559605..4559805 (-) 201 WP_214378795.1 DNA gyrase inhibitor YacG -
  ACETK7_RS21200 (ACETK7_21200) coaE 4559802..4560425 (-) 624 WP_214378793.1 dephospho-CoA kinase -
  ACETK7_RS21205 (ACETK7_21205) pilD 4560422..4561294 (-) 873 WP_214378791.1 A24 family peptidase Machinery gene
  ACETK7_RS21210 (ACETK7_21210) pilC 4561296..4562513 (-) 1218 WP_214378789.1 type II secretion system F family protein Machinery gene
  ACETK7_RS21215 (ACETK7_21215) pilB 4562516..4564216 (-) 1701 WP_214378787.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACETK7_RS21220 (ACETK7_21220) pilA 4564442..4564852 (+) 411 WP_214378785.1 pilin Machinery gene
  ACETK7_RS21225 (ACETK7_21225) - 4565168..4565521 (+) 354 WP_214378783.1 BON domain-containing protein -
  ACETK7_RS21230 (ACETK7_21230) - 4565746..4566042 (-) 297 WP_214384932.1 DUF2845 domain-containing protein -
  ACETK7_RS21235 (ACETK7_21235) - 4566042..4568993 (-) 2952 WP_214378781.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13813.12 Da        Isoelectric Point: 8.8451

>NTDB_id=1050229 ACETK7_RS21220 WP_214378785.1 4564442..4564852(+) (pilA) [Pseudomonas hormoni strain G20-18_CNT1]
MNTQKGFTLIELLIVVAIIGILASVALPQYSKYQSRAKVTAGLAEISALKVPYEDIMNQGTTPTLALLGVSATTGNCTIA
VAGTITTGVGSIGCTILNANGQVAGKTITLSRALAGGWTCKSTVPKEYAPNGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1050229 ACETK7_RS21220 WP_214378785.1 4564442..4564852(+) (pilA) [Pseudomonas hormoni strain G20-18_CNT1]
ATGAATACTCAAAAAGGTTTTACCCTGATCGAGCTGTTGATCGTGGTGGCGATCATCGGGATTCTGGCGTCGGTTGCGCT
GCCGCAGTATTCGAAGTATCAGTCGCGGGCGAAGGTCACCGCCGGTCTTGCTGAAATATCGGCATTGAAGGTCCCCTACG
AAGACATTATGAATCAAGGCACCACCCCCACCCTCGCTCTGCTCGGCGTTAGCGCGACCACAGGCAATTGCACGATTGCG
GTGGCGGGCACCATCACTACTGGTGTTGGCAGTATTGGCTGCACGATCCTCAATGCAAACGGTCAGGTGGCCGGCAAGAC
CATAACGCTTTCGCGGGCGCTGGCCGGTGGCTGGACTTGTAAATCGACCGTACCCAAAGAGTACGCGCCTAACGGTTGCC
CTGGCGTCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

49.635

100

0.5
  comP Acinetobacter baylyi ADP1

43.919

100

0.478
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.588

100

0.456
  pilA Vibrio cholerae C6706

42.958

100

0.449
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.958

100

0.449
  pilA Vibrio cholerae strain A1552

42.958

100

0.449
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.963

99.265

0.426
  pilA Pseudomonas aeruginosa PAK

39.189

100

0.426
  pilA Vibrio parahaemolyticus RIMD 2210633

40.602

97.794

0.397
  pilA Ralstonia pseudosolanacearum GMI1000

36.986

100

0.397
  pilA2 Legionella pneumophila str. Paris

38.406

100

0.39
  pilA2 Legionella pneumophila strain ERS1305867

38.406

100

0.39
  pilA/pilA1 Eikenella corrodens VA1

37.41

100

0.382
  pilA Haemophilus influenzae Rd KW20

34.932

100

0.375
  pilE Neisseria gonorrhoeae MS11

37.984

94.853

0.36

Multiple sequence alignment