Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACETK7_RS21215 Genome accession   NZ_CP169287
Coordinates   4562516..4564216 (-) Length   566 a.a.
NCBI ID   WP_214378787.1    Uniprot ID   -
Organism   Pseudomonas hormoni strain G20-18_CNT1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4557516..4569216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACETK7_RS21185 (ACETK7_21185) - 4558121..4558810 (+) 690 WP_214378799.1 energy-coupling factor ABC transporter permease -
  ACETK7_RS21190 (ACETK7_21190) - 4558868..4559203 (-) 336 WP_214378797.1 hypothetical protein -
  ACETK7_RS21195 (ACETK7_21195) yacG 4559605..4559805 (-) 201 WP_214378795.1 DNA gyrase inhibitor YacG -
  ACETK7_RS21200 (ACETK7_21200) coaE 4559802..4560425 (-) 624 WP_214378793.1 dephospho-CoA kinase -
  ACETK7_RS21205 (ACETK7_21205) pilD 4560422..4561294 (-) 873 WP_214378791.1 A24 family peptidase Machinery gene
  ACETK7_RS21210 (ACETK7_21210) pilC 4561296..4562513 (-) 1218 WP_214378789.1 type II secretion system F family protein Machinery gene
  ACETK7_RS21215 (ACETK7_21215) pilB 4562516..4564216 (-) 1701 WP_214378787.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACETK7_RS21220 (ACETK7_21220) pilA 4564442..4564852 (+) 411 WP_214378785.1 pilin Machinery gene
  ACETK7_RS21225 (ACETK7_21225) - 4565168..4565521 (+) 354 WP_214378783.1 BON domain-containing protein -
  ACETK7_RS21230 (ACETK7_21230) - 4565746..4566042 (-) 297 WP_214384932.1 DUF2845 domain-containing protein -
  ACETK7_RS21235 (ACETK7_21235) - 4566042..4568993 (-) 2952 WP_214378781.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62612.71 Da        Isoelectric Point: 6.1149

>NTDB_id=1050228 ACETK7_RS21215 WP_214378787.1 4562516..4564216(-) (pilB) [Pseudomonas hormoni strain G20-18_CNT1]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQSLRNRISLVTYLVQNKLVQSRQIAEIASEHFGIALLDLNCLNKEAQPKGL
VSEKLLRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLSDAIEKFFDSHSTGLEDMADVDLDG
VDIDSIDGHNQDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYESDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLGFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIDIPREALIKEGFPE
ERIGSFTIYEPVGCDQCNVGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1050228 ACETK7_RS21215 WP_214378787.1 4562516..4564216(-) (pilB) [Pseudomonas hormoni strain G20-18_CNT1]
ATGAATGACATCGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGCTCACTGATAAAAGCGCGCAACAGGC
GTATCAGCAGTCCCTTCGCAATCGCATCTCCCTGGTCACGTACCTGGTGCAGAACAAACTGGTGCAAAGCCGCCAGATTG
CCGAGATCGCATCGGAACACTTTGGCATAGCCTTGCTGGACCTCAACTGCCTGAACAAGGAGGCCCAGCCCAAGGGACTG
GTCAGCGAGAAACTGCTCCGTCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAGTTGTTCGTGGGCGTTTCCGA
CCCGACCAATCATCAAGCCATCAATGACATCCAGTTCAGCACCGGGCTGAATACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAGAAATTTTTCGACAGCCACAGTACCGGCCTGGAAGACATGGCCGACGTCGACCTCGATGGC
GTCGACATCGATTCGATCGATGGCCACAACCAGGACGCCATCGCCGGGCAAGACGCCGACGACGCGCCGGTGGTGCGCTT
TGTCCACAAGATGTTGCTCGACGCGATCAAGAGCGGCTCATCCGACCTGCACTTCGAACCCTACGAGAAAACCTACCGCG
TGCGGATGCGCACCGACGGCATGCTGCGTGAAGTCGCCAAGCCACCGATTCAACTGGCCAACCGCATCGCTGCGCGCCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGACGCAAACCGCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAATACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CCCAAATGGGCATCGACGCCCTTGGCTACGAGTCGGACCAGAAAGATCTGTACATGGCCGCGTTGAAGCAGCCGCAAGGG
ATGATTCTGGTGACCGGCCCCACCGGTTCGGGCAAGACCGTGTCGCTCTATACCGGGCTGAATATTCTCAACACTGTGGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAAG
GACTGGGTTTTGCCCAGGCACTGCGCTCGTTTTTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAAATTCGCGATCTG
GAAACCGCCGAAATTGCTATCAAGGCCGCCCAGACCGGGCACCTGGTGCTATCGACCCTGCACACCAACAGCGCGGCGGA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGATTCAACATCGCCACTTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCATTGCAAGAAACCCATCGATATTCCCCGCGAGGCATTGATCAAGGAAGGCTTCCCCGAG
GAACGCATTGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGACCAGTGCAATGTTGGCTACAAGGGGCGCGTGGGGAT
TTATGAAGTGGTGAAGAACACACCAGACCTGCAACGACTGATCATGGCCGAAGGCAATTCGCTGGAAATCGATATCCAGA
TGCGCAAGGACGGCTTCAACGATCTGCGTACTTCCGGGCTGATCAAGGCCATGCAGGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.437

99.47

0.581
  pilB Acinetobacter baumannii D1279779

58.259

99.47

0.58
  pilB Legionella pneumophila strain ERS1305867

56.184

100

0.562
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.628

98.41

0.498
  pilB Vibrio campbellii strain DS40M4

49.558

99.823

0.495
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.941

93.463

0.364

Multiple sequence alignment