Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACAW63_RS09665 Genome accession   NZ_CP166873
Coordinates   2098164..2098604 (+) Length   146 a.a.
NCBI ID   WP_371233297.1    Uniprot ID   -
Organism   Pseudomonas sp. QE6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2093164..2103604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAW63_RS09640 (ACAW63_09635) yacG 2093261..2093464 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  ACAW63_RS09645 (ACAW63_09640) coaE 2093461..2094069 (-) 609 WP_371233293.1 dephospho-CoA kinase -
  ACAW63_RS09650 (ACAW63_09645) pilD 2094135..2095007 (-) 873 WP_371233294.1 A24 family peptidase Machinery gene
  ACAW63_RS09655 (ACAW63_09650) pilC 2095013..2096230 (-) 1218 WP_371233295.1 type II secretion system F family protein Machinery gene
  ACAW63_RS09660 (ACAW63_09655) pilB 2096234..2097937 (-) 1704 WP_371233296.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAW63_RS09665 (ACAW63_09660) pilA 2098164..2098604 (+) 441 WP_371233297.1 pilin Machinery gene
  ACAW63_RS09670 (ACAW63_09665) - 2098668..2100524 (+) 1857 WP_371233299.1 hypothetical protein -
  ACAW63_RS09675 (ACAW63_09670) - 2100517..2102253 (+) 1737 WP_371233301.1 hypothetical protein -
  ACAW63_RS09685 (ACAW63_09680) galE 2102424..2103446 (-) 1023 Protein_1898 UDP-glucose 4-epimerase GalE -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14676.71 Da        Isoelectric Point: 8.4770

>NTDB_id=1035879 ACAW63_RS09665 WP_371233297.1 2098164..2098604(+) (pilA) [Pseudomonas sp. QE6]
MRAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLSLASAAKTTVAENAATGTSPLSLGWTAPATTANVSSV
SVDGATGAITVVYTTAAGNGKIVLKPNSAGSALSAGTVPSTSISWGCKTADGSDLDDKYRPANCRG

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1035879 ACAW63_RS09665 WP_371233297.1 2098164..2098604(+) (pilA) [Pseudomonas sp. QE6]
ATGAGAGCTCAAAAAGGCTTTACGCTGATCGAACTGATGATCGTGGTAGCGATCATCGGCATCCTGGCCGCCATCGCTAT
TCCGGCTTACCAGGACTACACCATTCGTGCTCGTGTGACCGAAGGTCTGTCCCTGGCTTCCGCCGCCAAGACCACTGTTG
CTGAAAACGCCGCTACCGGTACCTCCCCGCTGAGCCTGGGCTGGACTGCTCCGGCCACCACTGCGAACGTCAGCTCTGTC
AGTGTAGATGGCGCCACCGGTGCTATCACTGTCGTGTACACCACTGCAGCAGGCAACGGCAAGATCGTCCTGAAGCCGAA
CTCCGCAGGTTCTGCACTGAGCGCCGGCACCGTTCCGAGCACTTCGATCTCCTGGGGCTGCAAGACCGCTGATGGCTCTG
ACCTGGACGACAAGTATCGCCCGGCTAACTGCCGCGGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.088

100

0.589
  pilA2 Legionella pneumophila str. Paris

55.034

100

0.562
  pilA2 Legionella pneumophila strain ERS1305867

54.362

100

0.555
  comP Acinetobacter baylyi ADP1

46.154

100

0.493
  pilE Neisseria gonorrhoeae strain FA1090

38.788

100

0.438
  pilE Neisseria gonorrhoeae MS11

37.126

100

0.425
  pilA Acinetobacter baumannii strain A118

41.611

100

0.425
  pilA Pseudomonas aeruginosa PAK

38.037

100

0.425
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.397

100

0.418
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.816

100

0.404
  pilA Haemophilus influenzae 86-028NP

39.333

100

0.404
  pilA Vibrio cholerae C6706

38.158

100

0.397
  pilA Vibrio cholerae strain A1552

38.158

100

0.397
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.158

100

0.397
  pilA/pilA1 Eikenella corrodens VA1

36.306

100

0.39

Multiple sequence alignment