Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAW63_RS09660 Genome accession   NZ_CP166873
Coordinates   2096234..2097937 (-) Length   567 a.a.
NCBI ID   WP_371233296.1    Uniprot ID   -
Organism   Pseudomonas sp. QE6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2091234..2102937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAW63_RS09625 (ACAW63_09620) - 2091592..2092059 (+) 468 WP_371233288.1 FAD/FMN-containing dehydrogenase -
  ACAW63_RS09630 (ACAW63_09625) - 2092056..2092745 (+) 690 WP_371233291.1 energy-coupling factor ABC transporter permease -
  ACAW63_RS09635 (ACAW63_09630) - 2092852..2093067 (+) 216 WP_371233292.1 hypothetical protein -
  ACAW63_RS09640 (ACAW63_09635) yacG 2093261..2093464 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  ACAW63_RS09645 (ACAW63_09640) coaE 2093461..2094069 (-) 609 WP_371233293.1 dephospho-CoA kinase -
  ACAW63_RS09650 (ACAW63_09645) pilD 2094135..2095007 (-) 873 WP_371233294.1 A24 family peptidase Machinery gene
  ACAW63_RS09655 (ACAW63_09650) pilC 2095013..2096230 (-) 1218 WP_371233295.1 type II secretion system F family protein Machinery gene
  ACAW63_RS09660 (ACAW63_09655) pilB 2096234..2097937 (-) 1704 WP_371233296.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAW63_RS09665 (ACAW63_09660) pilA 2098164..2098604 (+) 441 WP_371233297.1 pilin Machinery gene
  ACAW63_RS09670 (ACAW63_09665) - 2098668..2100524 (+) 1857 WP_371233299.1 hypothetical protein -
  ACAW63_RS09675 (ACAW63_09670) - 2100517..2102253 (+) 1737 WP_371233301.1 hypothetical protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62736.85 Da        Isoelectric Point: 5.4768

>NTDB_id=1035878 ACAW63_RS09660 WP_371233296.1 2096234..2097937(-) (pilB) [Pseudomonas sp. QE6]
MNDQVVLSGLARQLVLSQLLDENSARQAQLQAQRNKLSLVTYLVQNKLVKSRPLTELAAEQFGIAFCDLGSLDRESFPKD
LVSEKLVRQHRVLPLWRRGNKLFVGLSDPTNHQAITDVQFSTGLTTEAILVEDDRLGDAIEKFFESATGGLDDLGDVDLE
GLDIEDGNLERKEEAIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLQEVAKPPVQLVSRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEEEQKQLYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPTCKKEHDVPRETLIHEGFP
EDRVGSFKLYAPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
INRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1035878 ACAW63_RS09660 WP_371233296.1 2096234..2097937(-) (pilB) [Pseudomonas sp. QE6]
ATGAACGATCAAGTCGTCCTGTCCGGCCTCGCCCGGCAGTTAGTGCTGTCTCAACTTCTCGATGAGAACAGCGCGCGGCA
GGCTCAACTACAAGCGCAACGCAACAAGCTGTCGCTAGTGACCTATCTGGTTCAGAACAAGCTGGTGAAAAGCCGCCCTC
TGACAGAACTGGCCGCCGAGCAATTCGGCATCGCCTTCTGTGATCTGGGCAGTCTGGACCGGGAGAGCTTCCCCAAGGAC
CTGGTCAGTGAGAAGCTCGTGCGCCAGCATCGCGTCCTCCCCTTGTGGAGGCGAGGCAACAAGCTCTTCGTCGGCCTTTC
GGATCCGACCAATCACCAGGCGATCACTGACGTCCAGTTCAGTACAGGACTGACCACCGAAGCCATTCTGGTGGAGGATG
ACCGGCTCGGCGACGCCATCGAAAAGTTCTTCGAGAGCGCGACCGGCGGCCTGGACGACCTGGGCGATGTCGATCTGGAA
GGCCTTGACATCGAAGACGGCAACCTCGAGCGCAAGGAGGAGGCCATCGGCGGTGACGCCGACGACGCCCCGGTGGTGCG
TTTCGTGAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCGTCCGACCTGCACTTCGAGCCCTATGAAAAGATATACA
GGGTTCGCTTCCGCACCGATGGCATGCTCCAGGAGGTGGCCAAGCCGCCAGTCCAACTGGTCAGCCGTATCTCGGCGCGC
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAGCGCCGCAAACCCCAGGACGGCCGCATCAAGATGCGCGTGTCCAAGAG
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCCACGCTGTGGGGTGAGAAAATCGTGATGCGTATCCTCGACTCGGCCA
GCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGAGCAGAAACAACTGTATCTCGCAGCACTGAAGCAGCCGCAA
GGCATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTATCCCTCTATACCGGTCTGAACATCCTCAATACGCC
GGACATCAATATCTCCACCGCCGAAGACCCGGTAGAGATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAC
AGGGCCTGGACTTCGCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAGATCCGCGAC
CTGGAGACGGCGGAAATCGCCATCAAGGCCGCGCAGACCGGACACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACTCTGACGCGCCTGCTGAACATGGGGGTTGCCTCTTTCAACCTCGCCACTTCGACTAACCTGATCATTGCCCAGC
GCCTGGCACGCAAACTCTGTCCGACCTGCAAGAAGGAACACGACGTACCGCGCGAAACCTTGATCCACGAAGGCTTTCCG
GAAGACCGGGTCGGCTCGTTCAAGCTCTACGCCCCGGTCGGCTGCGAAAACTGCAAGAACGGTTATAAGGGGCGTGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTACAGCGGATTATCATGGAAGAAGGCAATTCCATTCAGATCGCAGAAC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCGGGCCTGCTGAAAGCCATGCAGGGCATTACCAGCCTCGAGGAA
ATCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Acinetobacter baumannii D1279779

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.547
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.845

95.591

0.496
  pilB Vibrio campbellii strain DS40M4

51.201

95.414

0.489
  pilB Vibrio cholerae strain A1552

51.992

92.945

0.483
  pilF Thermus thermophilus HB27

37.805

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.4

94.004

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

38.795

93.651

0.363

Multiple sequence alignment