Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACAW63_RS09655 Genome accession   NZ_CP166873
Coordinates   2095013..2096230 (-) Length   405 a.a.
NCBI ID   WP_371233295.1    Uniprot ID   -
Organism   Pseudomonas sp. QE6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2090013..2101230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAW63_RS09610 (ACAW63_09605) - 2090182..2090352 (-) 171 WP_371233285.1 DUF3094 family protein -
  ACAW63_RS09615 (ACAW63_09610) - 2090435..2091064 (-) 630 WP_343574954.1 DUF1780 domain-containing protein -
  ACAW63_RS09620 (ACAW63_09615) - 2091173..2091589 (+) 417 WP_371233287.1 GNAT family N-acetyltransferase -
  ACAW63_RS09625 (ACAW63_09620) - 2091592..2092059 (+) 468 WP_371233288.1 FAD/FMN-containing dehydrogenase -
  ACAW63_RS09630 (ACAW63_09625) - 2092056..2092745 (+) 690 WP_371233291.1 energy-coupling factor ABC transporter permease -
  ACAW63_RS09635 (ACAW63_09630) - 2092852..2093067 (+) 216 WP_371233292.1 hypothetical protein -
  ACAW63_RS09640 (ACAW63_09635) yacG 2093261..2093464 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  ACAW63_RS09645 (ACAW63_09640) coaE 2093461..2094069 (-) 609 WP_371233293.1 dephospho-CoA kinase -
  ACAW63_RS09650 (ACAW63_09645) pilD 2094135..2095007 (-) 873 WP_371233294.1 A24 family peptidase Machinery gene
  ACAW63_RS09655 (ACAW63_09650) pilC 2095013..2096230 (-) 1218 WP_371233295.1 type II secretion system F family protein Machinery gene
  ACAW63_RS09660 (ACAW63_09655) pilB 2096234..2097937 (-) 1704 WP_371233296.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAW63_RS09665 (ACAW63_09660) pilA 2098164..2098604 (+) 441 WP_371233297.1 pilin Machinery gene
  ACAW63_RS09670 (ACAW63_09665) - 2098668..2100524 (+) 1857 WP_371233299.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44276.93 Da        Isoelectric Point: 9.7454

>NTDB_id=1035877 ACAW63_RS09655 WP_371233295.1 2095013..2096230(-) (pilC) [Pseudomonas sp. QE6]
MAEKALRTSVFTFEGTDRKGGKIKGELSGTSMALVKAQLRKQGINPTKVRKKGISLFGKGKAIRPMDIALFTRQMATMMG
SGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVASGNSLANSLRKKPMYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFSSFGAELPAFTLMVISLSETLQQWWFIFLIAIFAISFALR
EAHKRSEKFRDFIDRSILKLPIVGDILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVTRIKQDVSSGT
QLNFSMRTTGVFPSMAIQMAAIGEESGSLDAMLDKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1035877 ACAW63_RS09655 WP_371233295.1 2095013..2096230(-) (pilC) [Pseudomonas sp. QE6]
ATGGCGGAAAAAGCATTGAGGACCAGTGTCTTTACTTTCGAAGGCACTGATCGCAAGGGCGGGAAGATCAAGGGGGAGCT
ATCTGGAACTAGTATGGCGCTGGTCAAGGCCCAGTTGCGCAAACAAGGTATCAACCCCACCAAGGTCCGCAAGAAAGGCA
TCTCGCTGTTCGGCAAGGGCAAGGCCATCCGGCCTATGGACATCGCCCTGTTCACCCGACAGATGGCAACAATGATGGGC
TCGGGTGTTCCGCTGCTGCAGTCCTTTGACATTATTGGCGAGGGTTTCGACAACCCGAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGAGGTTGCCTCGGGTAACAGCCTCGCCAATTCGCTGCGCAAGAAGCCTATGTACTTCGATGACCTGTACT
GCAATCTGGTGGATGCGGGCGAACAATCCGGCGCACTGGAGACCTTGCTGGACCGGGTCGCCACCTACAAGGAAAAAACC
GAGTCGCTCAAGGCCAAGATCAAGAAAGCAATGACCTACCCTATCGCAGTCATTGTCGTTGCGATCATCGTCTCCGCGAT
TCTTCTGATTAAAGTCGTGCCCCAATTCCAGTCGGTCTTCTCCAGTTTCGGGGCAGAACTTCCCGCTTTCACGCTAATGG
TTATTTCCCTATCGGAAACCCTGCAGCAATGGTGGTTCATCTTTCTGATAGCAATATTCGCCATATCCTTTGCCTTACGA
GAGGCCCACAAGCGCTCGGAGAAGTTCAGAGACTTCATTGATCGCTCAATTCTAAAGCTCCCCATTGTCGGCGACATCCT
CTACAAATCAGCCGTCGCCCGTTATGCCCGCACGCTCTCTACCACTTTCGCCGCAGGCGTTCCCCTGGTGGAGGCACTGG
ACTCGGTATCCGGCGCCACCGGCAACGTGGTATTCCGTAATGCGGTGACCAGGATCAAGCAGGACGTATCCTCCGGTACG
CAATTGAACTTCTCCATGCGCACCACCGGCGTGTTCCCGAGCATGGCCATCCAGATGGCCGCCATCGGCGAGGAATCCGG
CTCGCTGGACGCCATGCTCGACAAGGTCGCCAGCTATTACGAGGAAGAGGTCGACAATGCCGTCGATAACCTCACCACAC
TGATGGAGCCAATGATCATGGCCGTCCTGGGCGTCCTGGTTGGCGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAA
CTGGGCAACGTCGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.259

100

0.793
  pilC Acinetobacter baumannii D1279779

60.988

100

0.61
  pilC Acinetobacter baylyi ADP1

59.852

100

0.6
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria meningitidis 44/76-A

44.836

98.025

0.44
  pilG Neisseria gonorrhoeae MS11

44.584

98.025

0.437
  pilC Vibrio cholerae strain A1552

40.988

100

0.41
  pilC Vibrio campbellii strain DS40M4

39.899

97.778

0.39
  pilC Thermus thermophilus HB27

37.157

99.012

0.368

Multiple sequence alignment