Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUE72_RS06995 Genome accession   NZ_AP027365
Coordinates   1599789..1600832 (+) Length   347 a.a.
NCBI ID   WP_286272382.1    Uniprot ID   -
Organism   Thalassotalea hakodatensis strain PTE2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1594789..1605832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE72_RS06975 - 1597022..1597474 (-) 453 WP_286272376.1 DUF4426 domain-containing protein -
  QUE72_RS06980 - 1597549..1598097 (-) 549 WP_074498390.1 YggT family protein -
  QUE72_RS06985 proC 1598193..1599008 (-) 816 WP_286272377.1 pyrroline-5-carboxylate reductase -
  QUE72_RS06990 - 1599047..1599721 (-) 675 WP_286272379.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE72_RS06995 pilT 1599789..1600832 (+) 1044 WP_286272382.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE72_RS07000 pilU 1600842..1601969 (+) 1128 WP_286272384.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE72_RS07005 - 1602017..1602658 (-) 642 WP_286272385.1 NAD(P)H-binding protein -
  QUE72_RS07010 ruvX 1602661..1603095 (-) 435 WP_286272386.1 Holliday junction resolvase RuvX -
  QUE72_RS07015 - 1603095..1603652 (-) 558 WP_074498384.1 YqgE/AlgH family protein -
  QUE72_RS07020 gshB 1603669..1604622 (-) 954 WP_286272388.1 glutathione synthase -
  QUE72_RS07025 rsmE 1604640..1605386 (-) 747 WP_286272390.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38809.43 Da        Isoelectric Point: 5.6342

>NTDB_id=100880 QUE72_RS06995 WP_286272382.1 1599789..1600832(+) (pilT) [Thalassotalea hakodatensis strain PTE2]
MDITELLAFSVENNASDLHLSTGVPPLIRVDGDVRKLNIPAFEAKDVNALVYDIMNDRQRKEYEENMEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKVLSLDDLGCPDIFRTISENPRGLVLVTGPTGSGKSTTLAAMIDYINNMKHDHILTIED
PIEFVHENKMCLINQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLVKKVGGGRVASHEIMIGVPAIRNLIREDKVAQMYSAIQTGMQHGMQTMDQCL
QNLVNRGLITRQDAMEKAADKNQFQGY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=100880 QUE72_RS06995 WP_286272382.1 1599789..1600832(+) (pilT) [Thalassotalea hakodatensis strain PTE2]
ATGGATATTACCGAATTACTCGCATTTAGCGTAGAAAATAATGCTTCGGATCTACATCTTTCTACGGGTGTTCCACCTTT
AATTCGCGTGGATGGCGATGTACGTAAACTTAACATACCTGCCTTTGAAGCCAAAGACGTAAATGCTCTTGTTTATGACA
TTATGAACGATCGTCAACGAAAAGAATACGAAGAAAACATGGAAGTGGATTTTTCTTTTGAAGTTCCTAACTTAGCACGT
TTTAGGGTTAATGCCTTTAATCAAAACAGAGGTCCCGCAGCAGTATTCCGTACTATTCCTAGTAAAGTATTGTCGTTGGA
TGATTTAGGATGTCCTGATATTTTCCGTACCATTTCAGAAAACCCGCGCGGCTTAGTGTTAGTGACAGGGCCAACGGGTT
CGGGTAAGTCAACCACGTTAGCGGCGATGATCGACTACATTAATAACATGAAGCATGATCATATTCTTACCATTGAAGAT
CCAATCGAATTTGTTCATGAGAACAAAATGTGTTTGATCAACCAACGTGAAGTACATCGTGATACGTTAAGTTTTAACAA
TGCGTTGCGTTCAGCCTTACGTGAAGATCCTGACGTTATACTCGTTGGTGAGATGCGTGATTTAGAAACCATACGTTTAG
CAATGACCGCTGCAGAAACGGGTCATCTAGTATTTGGTACCTTACATACCACCTCTGCACCTAAAACCATTGACCGTATT
ATTGATGTATTCCCTGCAGAAGAAAAATCAATGGTGCGTTCAATGCTGTCAGAGTCGTTGCGTGCGGTTATTTCTCAAAC
GTTAGTGAAGAAAGTTGGTGGCGGTCGTGTTGCTTCACATGAAATTATGATCGGCGTACCGGCGATACGTAACCTTATTC
GTGAAGATAAAGTGGCGCAAATGTACTCTGCAATTCAAACGGGGATGCAACATGGCATGCAAACCATGGATCAATGCTTA
CAAAACTTAGTGAATCGTGGCTTGATCACTAGACAAGATGCAATGGAAAAAGCCGCTGATAAAAATCAATTTCAAGGGTA
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.135

0.769
  pilT Acinetobacter nosocomialis M2

77.326

99.135

0.767
  pilT Acinetobacter baumannii D1279779

77.326

99.135

0.767
  pilT Acinetobacter baumannii strain A118

77.326

99.135

0.767
  pilT Pseudomonas aeruginosa PAK

76.744

99.135

0.761
  pilT Acinetobacter baylyi ADP1

76.453

99.135

0.758
  pilT Legionella pneumophila strain Lp02

73.547

99.135

0.729
  pilT Legionella pneumophila strain ERS1305867

73.547

99.135

0.729
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

97.118

0.695
  pilT Vibrio cholerae strain A1552

71.513

97.118

0.695
  pilT Neisseria meningitidis 8013

69.795

98.271

0.686
  pilT Neisseria gonorrhoeae MS11

69.501

98.271

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

99.424

0.53
  pilU Pseudomonas stutzeri DSM 10701

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

41.329

99.712

0.412
  pilU Acinetobacter baylyi ADP1

39.193

100

0.392

Multiple sequence alignment