Detailed information NTG0100881

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QUE72_RS07000
• Genome accession: NZ_AP027365
• Coordinates: 1600842..1601969 (+)
• Length: 375 a.a.
• NCBI ID: WP_286272384.1
• Uniprot ID: -
• Organism: Thalassotalea hakodatensis strain PTE2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1595842..1606969

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUE72_RS06975	-	1597022..1597474 (-)	453	WP_286272376.1	DUF4426 domain-containing protein	-
QUE72_RS06980	-	1597549..1598097 (-)	549	WP_074498390.1	YggT family protein	-
QUE72_RS06985	proC	1598193..1599008 (-)	816	WP_286272377.1	pyrroline-5-carboxylate reductase	-
QUE72_RS06990	-	1599047..1599721 (-)	675	WP_286272379.1	YggS family pyridoxal phosphate-dependent enzyme	-
QUE72_RS06995	pilT	1599789..1600832 (+)	1044	WP_286272382.1	type IV pilus twitching motility protein PilT	Machinery gene
QUE72_RS07000	pilU	1600842..1601969 (+)	1128	WP_286272384.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QUE72_RS07005	-	1602017..1602658 (-)	642	WP_286272385.1	NAD(P)H-binding protein	-
QUE72_RS07010	ruvX	1602661..1603095 (-)	435	WP_286272386.1	Holliday junction resolvase RuvX	-
QUE72_RS07015	-	1603095..1603652 (-)	558	WP_074498384.1	YqgE/AlgH family protein	-
QUE72_RS07020	gshB	1603669..1604622 (-)	954	WP_286272388.1	glutathione synthase	-
QUE72_RS07025	rsmE	1604640..1605386 (-)	747	WP_286272390.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
QUE72_RS07030	-	1605653..1606633 (+)	981	WP_286272391.1	rhodanese-related sulfurtransferase	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 41418.31 Da
• Isoelectric Point: 5.5703

>NTDB_id=100881 QUE72_RS07000 WP_286272384.1 1600842..1601969(+) (pilU) [Thalassotalea hakodatensis strain PTE2]
MLRFHQYLEKMVQHDASDMFVTAKLPVSAKINGELTPIDETALSPEAALELVHNAMNEKQKKQFDQEKECNFAISIDDIG
RFRVSAFWQRDMAGMVIRRIVTEIPDSDDLGLPSVLKDVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNRNSRGHILTIE
DPVEFVHEHGKSMVTQREVGLDTESFDAALKSSLRQAPDVILIGEIRSQEIMEHALAFAETGHLCIATLHANNANQAIDR
IMHLVPADQHGKLLFDLALNLRGIIAQQLIPTRDGNGRVAAIEILLNSPYIAELIKKGEVGDIKEVMEKSTEQGMQTFDQ
ALFNLYQRGLINYADAIHHADSPNDLRLMIKLRSNDQGGSGSLSGVTIDGLDPKD

Nucleotide

• Length: 1128 bp

>NTDB_id=100881 QUE72_RS07000 WP_286272384.1 1600842..1601969(+) (pilU) [Thalassotalea hakodatensis strain PTE2]
ATGTTGAGATTTCATCAATACCTCGAAAAAATGGTGCAGCATGATGCATCCGATATGTTTGTGACCGCGAAGTTGCCGGT
TAGCGCGAAAATCAATGGTGAACTAACGCCTATTGATGAAACGGCATTAAGTCCGGAAGCGGCGTTAGAACTTGTTCATA
ATGCAATGAATGAAAAGCAGAAAAAGCAATTTGACCAAGAAAAGGAATGTAACTTTGCTATTTCCATTGATGATATAGGC
CGATTTCGTGTTTCTGCGTTTTGGCAGCGTGATATGGCGGGTATGGTTATTCGTCGTATTGTGACTGAAATTCCAGATTC
TGATGACTTAGGTTTACCGTCGGTGTTAAAAGATGTCGTGATGTCTAAACGTGGGTTAGTGTTATTTGTTGGTGGTACAG
GTACGGGTAAGTCAACCTCAATGGCTGCCTTGATTGGTTATCGAAACCGTAACTCTCGTGGCCATATACTGACCATTGAA
GATCCGGTGGAATTTGTCCACGAACACGGAAAATCGATGGTTACACAGCGTGAAGTAGGTTTAGACACGGAAAGCTTTGA
TGCGGCATTAAAAAGCTCTTTACGCCAAGCACCTGATGTTATTCTAATTGGTGAGATCAGAAGCCAAGAAATCATGGAAC
ATGCGCTGGCGTTCGCGGAGACAGGGCACTTATGTATTGCAACTCTTCATGCGAATAATGCGAACCAAGCAATTGACCGT
ATCATGCATTTAGTGCCTGCGGATCAACATGGTAAATTACTGTTTGATTTGGCGTTAAATCTTCGCGGCATTATTGCTCA
GCAATTAATTCCTACTCGAGATGGCAATGGCCGAGTTGCTGCAATCGAAATATTGCTGAACTCTCCTTATATCGCTGAAT
TAATAAAAAAAGGTGAAGTTGGCGATATTAAAGAAGTGATGGAAAAATCTACCGAGCAAGGTATGCAAACCTTTGATCAA
GCACTGTTTAATTTGTATCAACGTGGCTTAATTAATTACGCGGATGCGATACACCATGCTGATTCACCAAATGACCTACG
CTTAATGATTAAACTACGCAGCAATGATCAAGGTGGCAGTGGTTCATTGTCGGGCGTAACAATAGACGGTTTAGATCCAA
AAGATTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.905	95.2	0.589
pilU	Vibrio cholerae strain A1552	57.065	98.133	0.56
pilU	Acinetobacter baylyi ADP1	58.857	93.333	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.319	92	0.389