Detailed information NTG1007006

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABK833_RS02000
• Genome accession: NZ_CP157459
• Coordinates: 422159..423307 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa PA103
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 417159..428307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABK833_RS01975 (ABK833_01975)	-	418310..418606 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ABK833_RS01980 (ABK833_01980)	-	418603..419196 (-)	594	WP_003084544.1	YggT family protein	-
ABK833_RS01985 (ABK833_01985)	proC	419207..420028 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ABK833_RS01990 (ABK833_01990)	-	420040..420732 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABK833_RS01995 (ABK833_01995)	pilT	420947..421981 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABK833_RS02000 (ABK833_02000)	pilU	422159..423307 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ABK833_RS02005 (ABK833_02005)	czcD	423315..424214 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABK833_RS02010 (ABK833_02010)	-	424368..424772 (+)	405	WP_003084560.1	NINE protein	-
ABK833_RS02015 (ABK833_02015)	-	425015..426388 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ABK833_RS02020 (ABK833_02020)	-	426385..427569 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=1007006 ABK833_RS02000 WP_003110016.1 422159..423307(+) (pilU) [Pseudomonas aeruginosa PA103]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=1007006 ABK833_RS02000 WP_003110016.1 422159..423307(+) (pilU) [Pseudomonas aeruginosa PA103]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382