Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ABEF80_RS01835 Genome accession   NZ_CP156772
Coordinates   378704..379126 (+) Length   140 a.a.
NCBI ID   WP_404798982.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7970     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 379187..419247 378704..379126 flank 61


Gene organization within MGE regions


Location: 378704..419247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF80_RS01835 (ABEF80_01835) pilE 378704..379126 (+) 423 WP_404798982.1 pilin Machinery gene
  ABEF80_RS01840 (ABEF80_01840) - 379187..382810 (+) 3624 WP_347455484.1 fused MFS/spermidine synthase -
  ABEF80_RS01845 (ABEF80_01845) - 382926..384560 (+) 1635 WP_347455485.1 Wzy polymerase domain-containing protein -
  ABEF80_RS01850 (ABEF80_01850) bfr 384615..385079 (-) 465 WP_347456728.1 bacterioferritin -
  ABEF80_RS01855 (ABEF80_01855) - 385319..385513 (-) 195 WP_347453509.1 bacterioferritin-associated ferredoxin -
  ABEF80_RS01860 (ABEF80_01860) - 385702..386085 (-) 384 WP_347453510.1 RidA family protein -
  ABEF80_RS01865 (ABEF80_01865) - 386161..388263 (-) 2103 WP_347456729.1 HD domain-containing protein -
  ABEF80_RS01870 (ABEF80_01870) rpoZ 388476..388757 (-) 282 WP_034587719.1 DNA-directed RNA polymerase subunit omega -
  ABEF80_RS01875 (ABEF80_01875) gmk 388832..389455 (-) 624 WP_034587720.1 guanylate kinase -
  ABEF80_RS01880 (ABEF80_01880) ispH 389590..390540 (+) 951 WP_347456730.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ABEF80_RS01885 (ABEF80_01885) - 390704..391252 (+) 549 WP_347456731.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABEF80_RS01890 (ABEF80_01890) pilV 391249..391770 (+) 522 WP_218951679.1 type IV pilus modification protein PilV -
  ABEF80_RS01895 (ABEF80_01895) - 391767..392681 (+) 915 WP_180182064.1 PilW family protein -
  ABEF80_RS01900 (ABEF80_01900) - 392686..393426 (+) 741 WP_347456732.1 hypothetical protein -
  ABEF80_RS01905 (ABEF80_01905) - 393442..397077 (+) 3636 WP_347456733.1 hypothetical protein -
  ABEF80_RS01910 (ABEF80_01910) comE 397077..397580 (+) 504 WP_347456734.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ABEF80_RS01915 (ABEF80_01915) - 397574..398005 (+) 432 WP_347456735.1 type IV pilin protein -
  ABEF80_RS01920 (ABEF80_01920) rpsP 398126..398383 (+) 258 WP_034587736.1 30S ribosomal protein S16 -
  ABEF80_RS01925 (ABEF80_01925) rimM 398413..398961 (+) 549 WP_347456736.1 ribosome maturation factor RimM -
  ABEF80_RS01930 (ABEF80_01930) trmD 399003..399752 (+) 750 WP_347456186.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ABEF80_RS01935 (ABEF80_01935) rplS 399929..400300 (+) 372 WP_004787066.1 50S ribosomal protein L19 -
  ABEF80_RS01940 (ABEF80_01940) - 400362..401345 (-) 984 WP_034587797.1 triacylglycerol lipase -
  ABEF80_RS01945 (ABEF80_01945) - 401881..402912 (-) 1032 WP_347454469.1 lipase secretion chaperone -
  ABEF80_RS01950 (ABEF80_01950) truB 403068..403973 (+) 906 WP_347453523.1 tRNA pseudouridine(55) synthase TruB -
  ABEF80_RS01955 (ABEF80_01955) - 403996..404763 (+) 768 WP_347455492.1 TSUP family transporter -
  ABEF80_RS01960 (ABEF80_01960) - 404892..405354 (+) 463 Protein_372 hemerythrin domain-containing protein -
  ABEF80_RS01965 (ABEF80_01965) - 405409..405822 (-) 414 WP_347455493.1 hypothetical protein -
  ABEF80_RS01970 (ABEF80_01970) - 405894..406772 (-) 879 WP_347453527.1 EamA family transporter -
  ABEF80_RS01975 (ABEF80_01975) - 406956..407831 (-) 876 WP_000102417.1 IS982-like element ISAba825 family transposase -
  ABEF80_RS01980 (ABEF80_01980) - 408076..409227 (+) 1152 WP_347456737.1 hypothetical protein -
  ABEF80_RS01985 (ABEF80_01985) - 409350..409523 (+) 174 WP_347454463.1 hypothetical protein -
  ABEF80_RS01990 (ABEF80_01990) - 409739..410797 (-) 1059 WP_034586922.1 RNA-guided endonuclease TnpB family protein -
  ABEF80_RS01995 (ABEF80_01995) tnpA 410818..411231 (+) 414 WP_075167884.1 IS200/IS605 family transposase -
  ABEF80_RS02000 (ABEF80_02000) serA 411378..412610 (-) 1233 WP_034587753.1 phosphoglycerate dehydrogenase -
  ABEF80_RS02005 (ABEF80_02005) - 412965..414374 (+) 1410 WP_347454120.1 FAD-binding oxidoreductase -
  ABEF80_RS02010 (ABEF80_02010) - 414525..414899 (+) 375 WP_347454460.1 hypothetical protein -
  ABEF80_RS02015 (ABEF80_02015) - 415071..416300 (+) 1230 WP_347454121.1 MFS transporter -
  ABEF80_RS02020 (ABEF80_02020) - 416639..417868 (+) 1230 WP_347456840.1 transposase -
  ABEF80_RS02025 (ABEF80_02025) - 418018..419247 (+) 1230 WP_347455790.1 transposase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14146.18 Da        Isoelectric Point: 6.1734

>NTDB_id=1002592 ABEF80_RS01835 WP_404798982.1 378704..379126(+) (pilE) [Acinetobacter thermotolerans strain ANC 7970]
MQKGFTLIELMIVVAIIGILAAIAIPAYQDYIARSQVTEAVTLASGQKGAVTEVKSNTGAWPSDNATAGIAAASEITGKY
VAQVAVGASGVITATMNSSGVSTPVASGTIILTPSETNGSYTWVCTSSLSAKYLPAACRS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=1002592 ABEF80_RS01835 WP_404798982.1 378704..379126(+) (pilE) [Acinetobacter thermotolerans strain ANC 7970]
ATGCAAAAGGGTTTTACCCTTATTGAATTAATGATTGTTGTAGCAATTATCGGTATTTTGGCTGCGATTGCGATTCCTGC
ATACCAAGACTATATCGCTCGTTCACAAGTTACTGAAGCGGTAACATTAGCAAGTGGTCAAAAAGGTGCGGTAACTGAAG
TTAAATCTAATACTGGTGCTTGGCCAAGTGACAATGCGACTGCAGGAATTGCAGCTGCAAGTGAAATCACTGGAAAATAT
GTTGCACAAGTAGCAGTTGGTGCAAGTGGTGTAATTACTGCTACTATGAATAGTTCAGGCGTAAGCACTCCTGTTGCATC
TGGTACAATTATTTTGACACCATCTGAAACTAATGGTTCATATACTTGGGTTTGTACTTCTAGTCTATCTGCTAAATACT
TGCCAGCAGCTTGTCGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-24)

Pilin (bacterial filament) (Pilin)

(33-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

49.682

100

0.557
  pilE Neisseria gonorrhoeae MS11

48.734

100

0.55
  pilA2 Legionella pneumophila str. Paris

52.857

100

0.529
  pilA2 Legionella pneumophila strain ERS1305867

52.857

100

0.529
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.938

100

0.493
  comP Acinetobacter baylyi ADP1

44.898

100

0.471
  pilA Ralstonia pseudosolanacearum GMI1000

39.157

100

0.464
  pilA Pseudomonas aeruginosa PAK

42.484

100

0.464
  pilA Acinetobacter nosocomialis M2

51.667

85.714

0.443
  pilA Acinetobacter baumannii strain A118

42.361

100

0.436
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.571

100

0.436
  pilA/pilA1 Eikenella corrodens VA1

37.58

100

0.421
  pilA Vibrio campbellii strain DS40M4

44.186

92.143

0.407
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.416

97.857

0.386
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.371
  pilA Vibrio cholerae C6706

36.364

100

0.371
  pilA Vibrio cholerae strain A1552

36.364

100

0.371

Multiple sequence alignment