Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1367889..1368260 Replicon chromosome
Accession NZ_CP010816
Organism Escherichia coli strain BL21 (TaKaRa)

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag SR36_RS06890 Protein ID WP_001317028.1
Coordinates 1368066..1368260 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1367889..1368067 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SR36_RS06865 (SR36_06865) 1363844..1365217 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
SR36_RS06870 (SR36_06870) 1365346..1366281 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
SR36_RS23095 1366333..1367568 - 1236 WP_000040858.1 site-specific integrase -
SR36_RS06880 (SR36_06880) 1367570..1367785 - 216 WP_000079604.1 excisionase XisR -
SR36_RS06885 (SR36_06885) 1367864..1368073 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 1367889..1368067 + 179 - - Antitoxin
SR36_RS06890 (SR36_06890) 1368066..1368260 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
SR36_RS06895 (SR36_06895) 1368317..1369126 - 810 WP_000166319.1 recombination protein RecT -
SR36_RS06900 (SR36_06900) 1369119..1370218 - 1100 Protein_1311 PD-(D/E)XK nuclease-like domain-containing protein -
SR36_RS06905 (SR36_06905) 1370320..1370595 - 276 WP_000632297.1 protein RacC -
SR36_RS06910 (SR36_06910) 1370670..1370840 - 171 WP_001352098.1 conserved protein, Rac prophage -
SR36_RS06915 (SR36_06915) 1370840..1371061 - 222 WP_000560223.1 killing protein KilR -
SR36_RS06920 (SR36_06920) 1371503..1371991 + 489 WP_001312793.1 superinfection exclusion protein B -
SR36_RS22475 1371988..1372143 - 156 WP_001169151.1 YdaF family protein -
SR36_RS06925 (SR36_06925) 1372287..1372499 + 213 WP_012775985.1 phage lysis protein EssD -
SR36_RS06930 (SR36_06930) 1372504..1372848 + 345 WP_000193293.1 YdfR family protein -
SR36_RS06935 (SR36_06935) 1372814..1373086 - 273 WP_000370546.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1366333..1378398 12065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T52902 WP_001317028.1 NZ_CP010816:c1368260-1368066 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T52902 NZ_CP010816:c1368260-1368066 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT52902 NZ_CP010816:1367889-1368067 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References