Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2127579..2127950 Replicon chromosome
Accession NZ_CP014268
Organism Escherichia coli B strain C2566

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag C2566_RS10615 Protein ID WP_001317028.1
Coordinates 2127756..2127950 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2127579..2127757 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2566_RS10590 2123534..2124907 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
C2566_RS10595 2125036..2125971 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
C2566_RS23025 2126023..2127258 - 1236 WP_000040858.1 site-specific integrase -
C2566_RS10605 2127260..2127475 - 216 WP_000079604.1 excisionase XisR -
C2566_RS10610 2127554..2127763 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 2127579..2127757 + 179 NuclAT_0 - Antitoxin
- 2127579..2127757 + 179 NuclAT_0 - Antitoxin
- 2127579..2127757 + 179 NuclAT_0 - Antitoxin
- 2127579..2127757 + 179 NuclAT_0 - Antitoxin
C2566_RS10615 2127756..2127950 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
C2566_RS10620 2128007..2128816 - 810 WP_000166319.1 recombination protein RecT -
C2566_RS10625 2128809..2129908 - 1100 Protein_2010 PD-(D/E)XK nuclease-like domain-containing protein -
C2566_RS10630 2130010..2130285 - 276 WP_000632297.1 protein RacC -
C2566_RS10635 2130360..2130530 - 171 WP_001352098.1 conserved protein, Rac prophage -
C2566_RS10640 2130530..2130751 - 222 WP_000560223.1 killing protein KilR -
C2566_RS10645 2131193..2131681 + 489 WP_001312793.1 superinfection exclusion protein B -
C2566_RS10650 2131678..2131833 - 156 WP_001169151.1 YdaF family protein -
C2566_RS10655 2131977..2132189 + 213 WP_012775985.1 phage lysis protein EssD -
C2566_RS10660 2132194..2132538 + 345 WP_000193293.1 YdfR family protein -
C2566_RS10665 2132504..2132776 - 273 WP_000370546.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2126023..2138088 12065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T60467 WP_001317028.1 NZ_CP014268:c2127950-2127756 [Escherichia coli B]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T60467 NZ_CP014268:c2127950-2127756 [Escherichia coli B]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT60467 NZ_CP014268:2127579-2127757 [Escherichia coli B]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References