Detailed information of TA system TA170004

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1367889..1368260
• Replicon: chromosome
• Accession: NZ_CP060121
• Organism: Escherichia coli BL21

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: FFHJCLDM_RS06720
• Protein ID: WP_001317028.1
• Coordinates: 1368066..1368260 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1367889..1368067 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFHJCLDM_RS06695	1363844..1365217	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
FFHJCLDM_RS06700	1365346..1366281	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FFHJCLDM_RS06705	1366333..1367568	-	1236	WP_000040858.1	site-specific integrase	-
FFHJCLDM_RS06710	1367570..1367785	-	216	WP_000079604.1	excisionase XisR	-
FFHJCLDM_RS06715	1367864..1368073	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1367889..1368067	+	179	NuclAT_0	-	Antitoxin
-	1367889..1368067	+	179	NuclAT_0	-	Antitoxin
-	1367889..1368067	+	179	NuclAT_0	-	Antitoxin
-	1367889..1368067	+	179	NuclAT_0	-	Antitoxin
FFHJCLDM_RS06720	1368066..1368260	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FFHJCLDM_RS06725	1368317..1369126	-	810	WP_000166319.1	recombination protein RecT	-
FFHJCLDM_RS06730	1369119..1370218	-	1100	Protein_1313	PD-(D/E)XK nuclease-like domain-containing protein	-
FFHJCLDM_RS06735	1370320..1370595	-	276	WP_000632297.1	protein RacC	-
FFHJCLDM_RS06740	1370670..1370840	-	171	WP_001352098.1	conserved protein, Rac prophage	-
FFHJCLDM_RS06745	1370840..1371061	-	222	WP_000560223.1	killing protein KilR	-
FFHJCLDM_RS06750	1371503..1371991	+	489	WP_001312793.1	superinfection exclusion protein B	-
FFHJCLDM_RS06755	1371988..1372143	-	156	WP_001169151.1	YdaF family protein	-
FFHJCLDM_RS06760	1372287..1372499	+	213	WP_012775985.1	phage lysis protein EssD	-
FFHJCLDM_RS06765	1372504..1372848	+	345	WP_000193293.1	YdfR family protein	-
FFHJCLDM_RS06770	1372814..1373086	-	273	WP_000370546.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP010816 Escherichia coli strain BL21 (TaKaRa) Prophage TA052902 1366333..1378398 0	inside	Prophage	-	-	1366333..1378398	12065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T170004 WP_001317028.1 NZ_CP060121:c1368260-1368066 [Escherichia coli BL21]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T170004 NZ_CP076853:c2186129-2185794 [Rhizobium sp. WYJ-E13]
TTGCCAAGTGACAGAAACCGAGCGAAGCCCGCTTCCACCAAGCGGGCTTCGTTGCCGCGGCAATCAAGCCATACCAAACA
ATTTCAGAAAGATTGGGAGCGCCTTTCGGGGGCCGGCCGCTATGACATGGTCGCACTGAAGAAGGTCGTCGGTTTGATCG
TCGACAATGAAGGTCCTCTGCCGGCCGAGCTGAAAGATCACGAGCTGACGGGTGACTGGAAAGATCATCGAGAATGTCAC
ATCGGTGGTGACTTTCTGCTCATCTACTCCATAGACGACAAGAGGAATCTGGTCGTGTTCGTTAGTGCCGGTACCCACGC
CGAACTCTTTGAATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT170004 NZ_CP060121:1367889-1368067 [Escherichia coli BL21]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin