Detailed information of TA system TA157029

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1400022..1400393
• Replicon: chromosome
• Accession: NZ_CP053602
• Organism: Escherichia coli BL21(DE3)

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HO396_RS06915
• Protein ID: WP_001317028.1
• Coordinates: 1400199..1400393 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1400022..1400200 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO396_RS06890	1395977..1397350	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
HO396_RS06895	1397479..1398414	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HO396_RS06900	1398466..1399701	-	1236	WP_000040858.1	site-specific integrase	-
HO396_RS06905	1399703..1399918	-	216	WP_000079604.1	excisionase XisR	-
HO396_RS06910	1399997..1400206	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1400022..1400200	+	179	NuclAT_0	-	Antitoxin
-	1400022..1400200	+	179	NuclAT_0	-	Antitoxin
-	1400022..1400200	+	179	NuclAT_0	-	Antitoxin
-	1400022..1400200	+	179	NuclAT_0	-	Antitoxin
HO396_RS06915	1400199..1400393	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HO396_RS06920	1400450..1401259	-	810	WP_000166319.1	recombination protein RecT	-
HO396_RS06925	1401252..1402351	-	1100	Protein_1352	PD-(D/E)XK nuclease-like domain-containing protein	-
HO396_RS06930	1402453..1402728	-	276	WP_000632297.1	protein RacC	-
HO396_RS06935	1402803..1402973	-	171	WP_001352098.1	conserved protein, Rac prophage	-
HO396_RS06940	1402973..1403194	-	222	WP_000560223.1	killing protein KilR	-
HO396_RS06945	1403636..1404124	+	489	WP_001312793.1	superinfection exclusion protein B	-
HO396_RS06950	1404121..1404276	-	156	WP_001169151.1	YdaF family protein	-
HO396_RS06955	1404420..1404632	+	213	WP_012775985.1	phage lysis protein EssD	-
HO396_RS06960	1404637..1404981	+	345	WP_000193293.1	YdfR family protein	-
HO396_RS06965	1404947..1405219	-	273	WP_000370546.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP010816 Escherichia coli strain BL21 (TaKaRa) Prophage TA052902 1366333..1378398 0	inside	Prophage	-	-	1398466..1410531	12065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T157029 WP_001317028.1 NZ_CP053602:c1400393-1400199 [Escherichia coli BL21(DE3)]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T157029 NZ_CP069075:c762477-762040 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT157029 NZ_CP053602:1400022-1400200 [Escherichia coli BL21(DE3)]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin