Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1380907..1381278 Replicon chromosome
Accession NZ_CP020368
Organism Escherichia coli strain BLR(DE3)

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag B5762_RS07270 Protein ID WP_001317028.1
Coordinates 1381084..1381278 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1380907..1381085 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5762_RS07245 1376862..1378235 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
B5762_RS07250 1378364..1379299 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
B5762_RS07255 1379351..1380586 - 1236 WP_000040858.1 site-specific integrase -
B5762_RS07260 1380588..1380803 - 216 WP_000079604.1 excisionase XisR -
B5762_RS07265 1380882..1381091 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 1380907..1381085 + 179 NuclAT_0 - Antitoxin
- 1380907..1381085 + 179 NuclAT_0 - Antitoxin
- 1380907..1381085 + 179 NuclAT_0 - Antitoxin
- 1380907..1381085 + 179 NuclAT_0 - Antitoxin
B5762_RS07270 1381084..1381278 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
B5762_RS07275 1381335..1382144 - 810 WP_000166319.1 recombination protein RecT -
B5762_RS07280 1382137..1383236 - 1100 Protein_1337 PD-(D/E)XK nuclease-like domain-containing protein -
B5762_RS07285 1383338..1383613 - 276 WP_000632297.1 protein RacC -
B5762_RS07290 1383688..1383858 - 171 WP_001352098.1 conserved protein, Rac prophage -
B5762_RS07295 1383858..1384079 - 222 WP_000560223.1 killing protein KilR -
B5762_RS07305 1384521..1385009 + 489 WP_001312793.1 superinfection exclusion protein B -
B5762_RS07310 1385006..1385161 - 156 WP_001169151.1 YdaF family protein -
B5762_RS07320 1385305..1385517 + 213 WP_012775985.1 phage lysis protein EssD -
B5762_RS07325 1385522..1385866 + 345 WP_000193293.1 YdfR family protein -
B5762_RS07330 1385832..1386104 - 273 WP_000370546.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1379351..1390017 10666


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T74418 WP_001317028.1 NZ_CP020368:c1381278-1381084 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T74418 NZ_CP020368:c1381278-1381084 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT74418 NZ_CP020368:1380907-1381085 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References