Detailed information of TA system TA157003

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1369186..1369557
• Replicon: chromosome
• Accession: NZ_CP053601
• Organism: Escherichia coli BL21

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HO397_RS06715
• Protein ID: WP_001317028.1
• Coordinates: 1369363..1369557 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1369186..1369364 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO397_RS06690	1365141..1366514	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
HO397_RS06695	1366643..1367578	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HO397_RS06700	1367630..1368865	-	1236	WP_000040858.1	site-specific integrase	-
HO397_RS06705	1368867..1369082	-	216	WP_000079604.1	excisionase XisR	-
HO397_RS06710	1369161..1369370	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1369186..1369364	+	179	NuclAT_0	-	Antitoxin
-	1369186..1369364	+	179	NuclAT_0	-	Antitoxin
-	1369186..1369364	+	179	NuclAT_0	-	Antitoxin
-	1369186..1369364	+	179	NuclAT_0	-	Antitoxin
HO397_RS06715	1369363..1369557	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HO397_RS06720	1369614..1370423	-	810	WP_000166319.1	recombination protein RecT	-
HO397_RS06725	1370416..1371515	-	1100	Protein_1312	PD-(D/E)XK nuclease-like domain-containing protein	-
HO397_RS06730	1371617..1371892	-	276	WP_000632297.1	protein RacC	-
HO397_RS06735	1371967..1372137	-	171	WP_001352098.1	conserved protein, Rac prophage	-
HO397_RS06740	1372137..1372358	-	222	WP_000560223.1	killing protein KilR	-
HO397_RS06745	1372800..1373288	+	489	WP_001312793.1	superinfection exclusion protein B	-
HO397_RS06750	1373285..1373440	-	156	WP_001169151.1	YdaF family protein	-
HO397_RS06755	1373584..1373796	+	213	WP_012775985.1	phage lysis protein EssD	-
HO397_RS06760	1373801..1374145	+	345	WP_000193293.1	YdfR family protein	-
HO397_RS06765	1374111..1374383	-	273	WP_000370546.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP010816 Escherichia coli strain BL21 (TaKaRa) Prophage TA052902 1366333..1378398 0.002	inside	Prophage	-	-	1366643..1379695	13052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T157003 WP_001317028.1 NZ_CP053601:c1369557-1369363 [Escherichia coli BL21]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T157003 NZ_CP069074:c3135398-3135042 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT157003 NZ_CP053601:1369186-1369364 [Escherichia coli BL21]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin