23934

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1398718..1399089 Replicon chromosome
Accession NC_012892
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name ralR Uniprot ID F4VC37
Locus tag B21_RS07095 Protein ID WP_001317028.1
Coordinates 1398895..1399089 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1398718..1398896 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B21_RS07070 1394673..1396046 + 1374 WP_000123737.1 ATP-dependent RNA helicase DbpA -
B21_RS07075 1396175..1397110 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
B21_RS23305 1397162..1398397 - 1236 WP_000040858.1 site-specific integrase -
B21_RS07085 1398399..1398614 - 216 WP_000079604.1 excisionase XisR -
B21_RS07090 1398693..1398902 - 210 WP_000276809.1 double-strand break reduction protein RcbA -
- 1398718..1398896 + 179 NuclAT_0 - Antitoxin
- 1398718..1398896 + 179 NuclAT_0 - Antitoxin
- 1398718..1398896 + 179 NuclAT_0 - Antitoxin
- 1398718..1398896 + 179 NuclAT_0 - Antitoxin
B21_RS07095 1398895..1399089 - 195 WP_001317028.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
B21_RS07100 1399146..1399955 - 810 WP_000166319.1 recombination protein RecT -
B21_RS07105 1399948..1401047 - 1100 Protein_1349 PD-(D/E)XK nuclease-like domain-containing protein -
B21_RS07110 1401149..1401424 - 276 WP_000632297.1 protein RacC -
B21_RS07115 1401499..1401669 - 171 WP_001352098.1 conserved protein, Rac prophage -
B21_RS07120 1401669..1401890 - 222 WP_000560223.1 killing protein KilR -
B21_RS07125 1402332..1402820 + 489 WP_001312793.1 superinfection exclusion protein B -
B21_RS22665 1402817..1402972 - 156 WP_001169151.1 YdaF family protein -
B21_RS07130 1403116..1403328 + 213 WP_012775985.1 phage lysis protein EssD -
B21_RS07135 1403333..1403677 + 345 WP_000193293.1 YdfR family protein -
B21_RS07140 1403643..1403915 - 273 WP_000370546.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1397162..1409227 12065


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-54)


Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7018.89 Da        Isoelectric Point: 8.9538

>T23934 WP_001317028.1 NC_012892:c1399089-1398895 [Escherichia coli BL21(DE3)]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Download         Length: 195 bp

>T23934 NC_012892:c1399089-1398895 [Escherichia coli BL21(DE3)]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 179 bp

>AT23934 NC_012892:1398718-1398896 [Escherichia coli BL21(DE3)]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LHV7

Antitoxin

Download structure file

References