Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 1923028..1923399 Replicon chromosome
Accession NC_002695
Organism Escherichia coli O157:H7 str. Sakai
T1TAdb ID TA04748

Toxin (Protein)


Gene name ralR Uniprot ID -
Locus tag - Protein ID -
Coordinates 1923205..1923399 (-) Length 65 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 1923028..1923206 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECs_1927 1919120..1920493 + 1374 NP_309954.1 ATP-dependent RNA helicase -
ECs_1928 1920622..1921557 - 936 NP_309955.1 tRNA s(2)C32 thioltransferase -
ECs_1929 1921609..1922844 - 1236 NP_309956.1 integrase -
ECs_1930 1922846..1923061 - 216 NP_309957.2 excisionase -
- 1923028..1923206 + 179 - - Antitoxin
- 1923205..1923399 - 195 - - Toxin
ECs_1933 1923592..1924401 - 810 NP_309960.1 recombination and repair protein -
ECs_1934 1924394..1926994 - 2601 NP_309961.1 exonuclease VIII -
ECs_1935 1927096..1927371 - 276 NP_309962.1 hypothetical protein -
ECs_5726 1927446..1927616 - 171 YP_009502622.1 hypothetical protein -
ECs_1936 1927616..1927837 - 222 NP_309963.2 phage cell division inhibitor protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1921609..1970151 48542


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.



Sequences

Toxin        


Download         Length: 65 a.a.        Molecular weight: 7205.59 Da        Isoelectric Point: 11.5875

>T10219 - NC_002695:c1923399-1923205 [Escherichia coli O157:H7 str. Sakai]
TRTMKTNHWKWRCSCIKLPPLLKRKVALLLTGQDIQNLNRNQNAKKCSQVKKKQAFPESRK

Download         Length: 195 bp

>T10219 NC_002695:c1923399-1923205 [Escherichia coli O157:H7 str. Sakai]
ACTCGAACGATGAAATAAACGAACCACTGGAAATAATAATGGAGGTGTTCATGTATAAAATTACCGCCACTATTGAAAAG
GAAGGTGGCACTCCTACTAACTGGACAAGATATTCAAAATCTAAACTAACGAAATCAGAATGCGAAAAAATGCTCTCAGG
TAAAAAAGAAGCAGGCGTTTCCAGAGAGCAGAAAG

Antitoxin


Download         Length: 179 bp

>AT10219 NC_002695:1923028-1923206 [Escherichia coli O157:H7 str. Sakai]
CCATCCACTCTTCATTAAAGATTACTTGTGCCATCGCATCACCTCTGGCAGGCGCCAATGTTAGACTTAAATTGACGCCC
GATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCACGAGGACTGAAGTTTCTCGCAATT
AAAATTTATCAGTTTTACT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Download structure file

References

(1) Yunxue Guo et al. (2014) RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Research 42(10):6448-62. [PubMed:24748661] experimental


experimental experimental literature