Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 327424..327795 Replicon chromosome
Accession NZ_CP027555
Organism Escherichia coli strain 2013C-3513

Toxin (Protein)


Gene name ralR Uniprot ID A0A0D6ZRD3
Locus tag C6991_RS02550 Protein ID WP_001443846.1
Coordinates 327646..327795 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 327424..327602 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6991_RS02525 323379..324752 + 1374 WP_032323876.1 ATP-dependent RNA helicase DbpA -
C6991_RS02530 324881..325816 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
C6991_RS02535 325868..327103 - 1236 WP_000040839.1 site-specific integrase -
C6991_RS02540 327105..327320 - 216 WP_000079604.1 excisionase XisR -
C6991_RS02545 327420..327608 - 189 WP_001302840.1 DUF1187 family protein -
- 327424..327602 + 179 NuclAT_0 - Antitoxin
- 327424..327602 + 179 NuclAT_0 - Antitoxin
- 327424..327602 + 179 NuclAT_0 - Antitoxin
- 327424..327602 + 179 NuclAT_0 - Antitoxin
C6991_RS02550 327646..327795 - 150 WP_001443846.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
C6991_RS02555 327851..328660 - 810 WP_000166313.1 recombination protein RecT -
C6991_RS02560 328653..331253 - 2601 WP_000105153.1 exodeoxyribonuclease VIII -
C6991_RS02565 331355..331630 - 276 WP_001344816.1 hypothetical protein -
C6991_RS02570 331705..331875 - 171 WP_001352098.1 conserved protein, Rac prophage -
C6991_RS02575 331875..332096 - 222 WP_000560223.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 325868..375068 49200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-48)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5383.12 Da        Isoelectric Point: 8.3398

>T99431 WP_001443846.1 NZ_CP027555:c327795-327646 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Download         Length: 150 bp

>T99431 NZ_CP027555:c327795-327646 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin


Download         Length: 179 bp

>AT99431 NZ_CP027555:327424-327602 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D6ZRD3

Antitoxin

Download structure file

References