Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2868849..2869220 Replicon chromosome
Accession NZ_CP062160
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name ralR Uniprot ID A0A0D6ZRD3
Locus tag IEM32_RS13830 Protein ID WP_001443846.1
Coordinates 2869071..2869220 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2868849..2869027 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS13800 2864601..2864771 + 171 WP_001625136.1 hypothetical protein -
IEM32_RS13805 2864804..2866177 + 1374 WP_000123746.1 ATP-dependent RNA helicase DbpA -
IEM32_RS13810 2866306..2867241 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
IEM32_RS13815 2867293..2868528 - 1236 WP_000040839.1 site-specific integrase -
IEM32_RS13820 2868530..2868745 - 216 WP_000079604.1 excisionase XisR -
IEM32_RS13825 2868845..2869033 - 189 WP_001302840.1 DUF1187 family protein -
- 2868849..2869027 + 179 NuclAT_0 - Antitoxin
- 2868849..2869027 + 179 NuclAT_0 - Antitoxin
- 2868849..2869027 + 179 NuclAT_0 - Antitoxin
- 2868849..2869027 + 179 NuclAT_0 - Antitoxin
IEM32_RS13830 2869071..2869220 - 150 WP_001443846.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
IEM32_RS13835 2869276..2870085 - 810 WP_000166313.1 recombination protein RecT -
IEM32_RS13840 2870078..2872678 - 2601 WP_000105140.1 exodeoxyribonuclease VIII -
IEM32_RS13845 2872780..2873055 - 276 WP_001344816.1 hypothetical protein -
IEM32_RS13850 2873130..2873300 - 171 WP_001352098.1 conserved protein, Rac prophage -
IEM32_RS13855 2873300..2873521 - 222 WP_000560223.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2855030..2916445 61415


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-48)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5383.12 Da        Isoelectric Point: 8.3398

>T173449 WP_001443846.1 NZ_CP062160:c2869220-2869071 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Download         Length: 150 bp

>T173449 NZ_CP080174:2254-2532 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 179 bp

>AT173449 NZ_CP062160:2868849-2869027 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D6ZRD3

Antitoxin

Download structure file

References