275452

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2574255..2574626 Replicon chromosome
Accession NZ_CP120944
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name ralR Uniprot ID A0A0D6ZRD3
Locus tag P7249_RS12945 Protein ID WP_001443846.1
Coordinates 2574477..2574626 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2574255..2574433 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P7249_RS12915 (2570007) 2570007..2570177 + 171 WP_001625136.1 protein YnaL -
P7249_RS12920 (2570210) 2570210..2571583 + 1374 WP_000123746.1 ATP-dependent RNA helicase DbpA -
P7249_RS12925 (2571712) 2571712..2572647 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
P7249_RS12930 (2572699) 2572699..2573934 - 1236 WP_000040839.1 site-specific integrase -
P7249_RS12935 (2573936) 2573936..2574151 - 216 WP_000079604.1 excisionase XisR -
- (2574255) 2574255..2574433 + 179 NuclAT_0 - Antitoxin
- (2574255) 2574255..2574433 + 179 NuclAT_0 - Antitoxin
- (2574255) 2574255..2574433 + 179 NuclAT_0 - Antitoxin
- (2574255) 2574255..2574433 + 179 NuclAT_0 - Antitoxin
P7249_RS12940 (2574251) 2574251..2574439 - 189 WP_001302840.1 DUF1187 family protein -
P7249_RS12945 (2574477) 2574477..2574626 - 150 WP_001443846.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
P7249_RS12950 (2574682) 2574682..2575491 - 810 WP_000166313.1 recombination protein RecT -
P7249_RS12955 (2575484) 2575484..2578084 - 2601 WP_000105140.1 exodeoxyribonuclease VIII -
P7249_RS12960 (2578186) 2578186..2578461 - 276 WP_001344816.1 hypothetical protein -
P7249_RS12965 (2578536) 2578536..2578706 - 171 WP_001352098.1 YdaE family protein -
P7249_RS12970 (2578706) 2578706..2578927 - 222 WP_000560223.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2565440..2621851 56411


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-48)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5383.12 Da        Isoelectric Point: 8.3398

>T275452 WP_001443846.1 NZ_CP120944:c2574626-2574477 [Escherichia coli O157:H7 str. EDL933]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Download         Length: 150 bp

Antitoxin


Download         Length: 179 bp

>AT275452 NZ_CP120944:2574255-2574433 [Escherichia coli O157:H7 str. EDL933]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D6ZRD3

Antitoxin

Download structure file

References