Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2573889..2574260 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name ralR Uniprot ID A0A0D6ZRD3
Locus tag NUE46_RS12930 Protein ID WP_001443846.1
Coordinates 2574111..2574260 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2573889..2574067 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS12900 (2569641) 2569641..2569811 + 171 WP_001625136.1 protein YnaL -
NUE46_RS12905 (2569844) 2569844..2571217 + 1374 WP_000123746.1 ATP-dependent RNA helicase DbpA -
NUE46_RS12910 (2571346) 2571346..2572281 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
NUE46_RS12915 (2572333) 2572333..2573568 - 1236 WP_000040839.1 site-specific integrase -
NUE46_RS12920 (2573570) 2573570..2573785 - 216 WP_000079604.1 excisionase XisR -
- (2573889) 2573889..2574067 + 179 NuclAT_0 - Antitoxin
- (2573889) 2573889..2574067 + 179 NuclAT_0 - Antitoxin
- (2573889) 2573889..2574067 + 179 NuclAT_0 - Antitoxin
- (2573889) 2573889..2574067 + 179 NuclAT_0 - Antitoxin
NUE46_RS12925 (2573885) 2573885..2574073 - 189 WP_001302840.1 DUF1187 family protein -
NUE46_RS12930 (2574111) 2574111..2574260 - 150 WP_001443846.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
NUE46_RS12935 (2574316) 2574316..2575125 - 810 WP_000166313.1 recombination protein RecT -
NUE46_RS12940 (2575118) 2575118..2577718 - 2601 WP_000105140.1 exodeoxyribonuclease VIII -
NUE46_RS12945 (2577820) 2577820..2578095 - 276 WP_001344816.1 hypothetical protein -
NUE46_RS12950 (2578170) 2578170..2578340 - 171 WP_001352098.1 YdaE family protein -
NUE46_RS12955 (2578340) 2578340..2578561 - 222 WP_000560223.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2560070..2621485 61415


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-48)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5383.12 Da        Isoelectric Point: 8.3398

>T257540 WP_001443846.1 NZ_CP104309:c2574260-2574111 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Download         Length: 150 bp

Antitoxin


Download         Length: 179 bp

>AT257540 NZ_CP104309:2573889-2574067 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D6ZRD3

Antitoxin

Download structure file

References