Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2638516..2638887 Replicon chromosome
Accession NZ_CP038363
Organism Escherichia coli O157:H7 strain F7349

Toxin (Protein)


Gene name ralR Uniprot ID A0A0D6ZRD3
Locus tag E4U70_RS13145 Protein ID WP_001443846.1
Coordinates 2638738..2638887 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2638516..2638694 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U70_RS13120 2634471..2635844 + 1374 WP_000123746.1 ATP-dependent RNA helicase DbpA -
E4U70_RS13125 2635973..2636908 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
E4U70_RS13130 2636960..2638195 - 1236 WP_000040839.1 site-specific integrase -
E4U70_RS13135 2638197..2638412 - 216 WP_000079604.1 excisionase XisR -
E4U70_RS13140 2638512..2638700 - 189 WP_001302840.1 DUF1187 family protein -
- 2638516..2638694 + 179 NuclAT_0 - Antitoxin
- 2638516..2638694 + 179 NuclAT_0 - Antitoxin
- 2638516..2638694 + 179 NuclAT_0 - Antitoxin
- 2638516..2638694 + 179 NuclAT_0 - Antitoxin
E4U70_RS13145 2638738..2638887 - 150 WP_001443846.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
E4U70_RS13150 2638943..2639752 - 810 WP_000166313.1 recombination protein RecT -
E4U70_RS13155 2639745..2642345 - 2601 WP_000105140.1 exodeoxyribonuclease VIII -
E4U70_RS13160 2642447..2642722 - 276 WP_001344816.1 hypothetical protein -
E4U70_RS13165 2642797..2642967 - 171 WP_001352098.1 conserved protein, Rac prophage -
E4U70_RS13170 2642967..2643188 - 222 WP_000560223.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2624697..2686112 61415


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-48)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5383.12 Da        Isoelectric Point: 8.3398

>T121991 WP_001443846.1 NZ_CP038363:c2638887-2638738 [Escherichia coli O157:H7]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Download         Length: 150 bp

>T121991 NZ_CP038363:c2638887-2638738 [Escherichia coli O157:H7]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin


Download         Length: 179 bp

>AT121991 NZ_CP038363:2638516-2638694 [Escherichia coli O157:H7]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D6ZRD3

Antitoxin

Download structure file

References