Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) ralRA/-
Location 2757929..2758300 Replicon chromosome
Accession NZ_CP028650
Organism Escherichia coli strain 124

Toxin (Protein)


Gene name ralR Uniprot ID A0A0D6ZRD3
Locus tag C9E53_RS14425 Protein ID WP_001443846.1
Coordinates 2758151..2758300 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name ralA
Locus tag -
Coordinates 2757929..2758107 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E53_RS28390 2753681..2753851 + 171 WP_001625136.1 hypothetical protein -
C9E53_RS14400 2753884..2755257 + 1374 WP_000123746.1 ATP-dependent RNA helicase DbpA -
C9E53_RS14405 2755386..2756321 - 936 WP_001157407.1 tRNA 2-thiocytidine(32) synthetase TtcA -
C9E53_RS14410 2756373..2757608 - 1236 WP_000040839.1 site-specific integrase -
C9E53_RS14415 2757610..2757825 - 216 WP_000079604.1 excisionase XisR -
C9E53_RS14420 2757925..2758113 - 189 WP_001302840.1 DUF1187 family protein -
- 2757929..2758107 + 179 NuclAT_0 - Antitoxin
- 2757929..2758107 + 179 NuclAT_0 - Antitoxin
- 2757929..2758107 + 179 NuclAT_0 - Antitoxin
- 2757929..2758107 + 179 NuclAT_0 - Antitoxin
C9E53_RS14425 2758151..2758300 - 150 WP_001443846.1 type I toxin-antitoxin system endodeoxyribonuclease toxin RalR Toxin
C9E53_RS14430 2758356..2759165 - 810 WP_000166313.1 recombination protein RecT -
C9E53_RS14435 2759158..2761758 - 2601 WP_000105140.1 exodeoxyribonuclease VIII -
C9E53_RS14440 2761860..2762135 - 276 WP_001344816.1 hypothetical protein -
C9E53_RS14445 2762210..2762380 - 171 WP_001352098.1 conserved protein, Rac prophage -
C9E53_RS14450 2762380..2762601 - 222 WP_000560223.1 killing protein KilR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2744122..2805525 61403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endodeoxyribonuclease toxin RalR

(6-48)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5383.12 Da        Isoelectric Point: 8.3398

>T102264 WP_001443846.1 NZ_CP028650:c2758300-2758151 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Download         Length: 150 bp

>T102264 NZ_CP028650:c2758300-2758151 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin


Download         Length: 179 bp

>AT102264 NZ_CP028650:2757929-2758107 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D6ZRD3

Antitoxin

Download structure file

References