Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS01219  |
| T6SS type | Type i4a |
| Strain | Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 |
| Replicon (RefSeq) | chromosome (NZ_CP032631) |
| Location | 3616254..3638573 |
| Function | anti-bacterial; anti-eukaryotic; anti-adverse environment |
| Comment | Western blot results revealed that tssM and tssJ were necessary for hemolytic co-regulatory protein secretion, indicating that they encode a functional T6SS |
| References | [1] Wang N, Han N, Tian R, Chen J, Gao X, Wu Z, Liu Y, Huang L. et al (2021) Role of the Type VI Secretion System in the Pathogenicity of Pseudomonas syringae pv. actinidiae, the Causative Agent of Kiwifruit Bacterial Canker.. Front Microbiol. 12:627785. [PudMed:33679650] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssI | TssJ | TssK | TssL |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| CN228_RS17535 | 3612641..3613213 | + | 573 | WP_003382802.1 | sarcosine oxidase | ||
| CN228_RS17540 | 3613427..3615367 | + | 1941 | WP_017684350.1 | methyl-accepting chemotaxis protein | ||
| CN228_RS17545 | 3615462..3615656 | - | 195 | pseudogene | LysR family transcriptional regulator | ||
| CN228_RS17550 | 3615651..3616208 | + | 558 | pseudogene | MBL fold metallo-hydrolase | ||
| T6CP015413 | TssA | CN228_RS17555 (tssA) | 3616254..3617354 | - | 1101 | WP_017684347.1 | type VI secretion system protein TssA |
| CN228_RS17560 | 3617351..3621165 | - | 3815 | pseudogene | type VI secretion system protein ImpL | ||
| T6CP015415 | TssL | CN228_RS17565 | 3621162..3621932 | - | 771 | WP_017684346.1 | DotU family type IV/VI secretion system protein |
| T6CP015416 | TssK | CN228_RS17570 (tssK) | 3621950..3623281 | - | 1332 | WP_017684345.1 | type VI secretion system baseplate subunit TssK |
| T6CP015417 | TssJ | CN228_RS17575 | 3623335..3623808 | - | 474 | WP_003382815.1 | hypothetical protein |
| T6CP015418 | TssB | CN228_RS17580 (tssB) | 3624009..3624560 | + | 552 | WP_017684344.1 | type VI secretion system contractile sheath small subunit |
| T6CP015419 | TssC | CN228_RS17585 (tssC) | 3624595..3626082 | + | 1488 | WP_003382817.1 | type VI secretion system contractile sheath large subunit |
| T6CP015420 | TssD | CN228_RS17590 | 3626159..3626656 | + | 498 | WP_003382818.1 | type VI secretion system tube protein Hcp |
| T6CP015421 | TssE | CN228_RS17595 (tssE) | 3626672..3627121 | + | 450 | WP_003382819.1 | type VI secretion system baseplate subunit TssE |
| T6CP015422 | TssF | CN228_RS17600 (tssF) | 3627105..3628901 | + | 1797 | WP_024533274.1 | type VI secretion system baseplate subunit TssF |
| T6CP015423 | TssG | CN228_RS17605 (tssG) | 3628865..3629884 | + | 1020 | WP_020312721.1 | type VI secretion system baseplate subunit TssG |
| CN228_RS17610 (tssH) | 3629884..3632446 | + | 2563 | pseudogene | type VI secretion system ATPase TssH | ||
| CN228_RS17620 | 3632748..3633925 | - | 1178 | WP_164706461.1 | IS3 family transposase | ||
| CN228_RS33105 | 3634010..3634369 | + | 360 | WP_158102008.1 | hypothetical protein | ||
| CN228_RS17625 | 3634366..3635945 | + | 1580 | WP_085991147.1 | IS3-like element ISPsy31 family transposase | ||
| CN228_RS17630 | 3636133..3636510 | + | 378 | WP_017699013.1 | hypothetical protein | ||
| T6CP015425 | TssI | CN228_RS17635 (tssI) | 3636567..3638573 | + | 2007 | WP_017704523.1 | type VI secretion system tip protein VgrG |
| CN228_RS17640 | 3638584..3639126 | + | 543 | WP_003376824.1 | toxin-antitoxin system YwqK family antitoxin | ||
| CN228_RS17645 | 3639134..3639529 | - | 396 | WP_003376826.1 | DUF4280 domain-containing protein | ||
| CN228_RS17650 | 3639652..3640050 | + | 399 | WP_024533390.1 | hypothetical protein | ||
| CN228_RS17655 | 3640111..3640596 | + | 486 | WP_003376830.1 | GNAT family N-acetyltransferase | ||
| CN228_RS17665 | 3640841..3642331 | - | 1491 | WP_024533391.1 | chemotaxis protein CheW |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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