Detailed information of component protein
Summary
| Component ID | T6CP015417 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01219  (Type i4a) |
| Strain | Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 |
| Replicon | chromosome |
| Sequence | Protein sequence (158 a.a.); Nucleotide sequence (474 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | CN228_RS17575 |
| Coordinate (Strand) | 3623335..3623808 (-) |
| NCBI ID | WP_003382815.1 |
| RefSeq | NZ_CP032631 |
| Uniprot ID | A0A0K8LW68_PSESF, A0A0Q0L1F9_PSESX, A0A261WDZ1_9PSED |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-11 | MTAKQFKYRQW |
| Transmembrane helix | 12-29 | IAVLLIAVVVAGCSLFGP |
| Outside | 30-157 | RIRIDGVTLDVALRANDDSPIAVDFVAVNDAGLLRQLSAMTADEWFAQRAQFQRDYPQQLYTWGMELVPGQLVESGVFPLQGKPALGLLAFAHYGTPGAHRLRLDDQRRIWLKFDSREMTLHDEKAIE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9954 | pos: 23-24. VAG-CS. | MTAKQFKYRQWIAVLLIAVVVAG |
Protein sequence: 158 a.a.
>T6CP015417 NZ_CP032631:c3623808-3623335 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssJ]
MTAKQFKYRQWIAVLLIAVVVAGCSLFGPRIRIDGVTLDVALRANDDSPIAVDFVAVNDAGLLRQLSAMTADEWFAQRAQ
FQRDYPQQLYTWGMELVPGQLVESGVFPLQGKPALGLLAFAHYGTPGAHRLRLDDQRRIWLKFDSREMTLHDEKAIE*
MTAKQFKYRQWIAVLLIAVVVAGCSLFGPRIRIDGVTLDVALRANDDSPIAVDFVAVNDAGLLRQLSAMTADEWFAQRAQ
FQRDYPQQLYTWGMELVPGQLVESGVFPLQGKPALGLLAFAHYGTPGAHRLRLDDQRRIWLKFDSREMTLHDEKAIE*
Nucleotide sequence: 474 bp
>T6CP015417 NZ_CP032631:c3623808-3623335 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssJ]
ATGACCGCGAAACAGTTCAAATATCGACAATGGATTGCCGTTTTGCTGATCGCGGTTGTGGTCGCAGGCTGTTCGCTTTT
TGGCCCCAGGATAAGGATCGACGGGGTCACACTGGACGTGGCTTTGCGGGCCAACGACGACTCGCCCATTGCGGTGGATT
TTGTGGCGGTCAACGATGCCGGATTGCTCCGCCAGCTGTCCGCAATGACTGCCGACGAGTGGTTTGCCCAGCGCGCCCAG
TTTCAGCGTGATTACCCTCAGCAACTGTATACGTGGGGAATGGAACTGGTTCCGGGGCAACTCGTCGAGTCAGGTGTCTT
CCCGCTGCAAGGCAAACCTGCGCTTGGCCTGTTGGCGTTTGCCCATTACGGCACGCCGGGGGCACACCGTTTGCGGCTGG
ATGATCAGCGCCGGATATGGTTGAAATTCGATAGCCGTGAAATGACCCTGCACGACGAAAAAGCGATTGAGTAG
ATGACCGCGAAACAGTTCAAATATCGACAATGGATTGCCGTTTTGCTGATCGCGGTTGTGGTCGCAGGCTGTTCGCTTTT
TGGCCCCAGGATAAGGATCGACGGGGTCACACTGGACGTGGCTTTGCGGGCCAACGACGACTCGCCCATTGCGGTGGATT
TTGTGGCGGTCAACGATGCCGGATTGCTCCGCCAGCTGTCCGCAATGACTGCCGACGAGTGGTTTGCCCAGCGCGCCCAG
TTTCAGCGTGATTACCCTCAGCAACTGTATACGTGGGGAATGGAACTGGTTCCGGGGCAACTCGTCGAGTCAGGTGTCTT
CCCGCTGCAAGGCAAACCTGCGCTTGGCCTGTTGGCGTTTGCCCATTACGGCACGCCGGGGGCACACCGTTTGCGGCTGG
ATGATCAGCGCCGGATATGGTTGAAATTCGATAGCCGTGAAATGACCCTGCACGACGAAAAAGCGATTGAGTAG