SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015423
Component protein type	TssG
T6SS ID (Type)	T6SS01219 (Type i4a)
Strain	Pseudomonas syringae pv. actinidiae str. Shaanxi_M228
Replicon	chromosome
Sequence	Protein sequence (340 a.a.); Nucleotide sequence (1020 bp)
Reference	[1] Wang N, Han N, Tian R, et al. Role of the Type VI Secretion System in the Pathogenicity of Pseudomonas syringae pv. actinidiae, the Causative Agent of Kiwifruit Bacterial Canker. Front Microbiol. 2021 Feb 19;12:627785. doi: 10.3389/fmicb.2021.627785. eCollection 2021. PMID: 33679650

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CN228_RS17605
Coordinate (Strand)	3628865..3629884 (+)
NCBI ID	WP_020312721.1
RefSeq	NZ_CP032631
Uniprot ID	UPI000356F77F
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	4.5e-75	20..316

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-339	METAGRQAAVSLSATLRQTPQAFELLQALLVLEREQPQAASLGTGTSPHAEAVRLRGPLTPVFASSQIESLVQEHDQQAVLTTPVFGLGGPDGPLPYAFQEWLQQRARAKDHAPAEFLDLFQHRLLSLLYRILRKHRIAAGYSVPSASPVQAQLRALTGLLPQPLQERQALPDAALLARSALFADGRRSLAGFAAIIRQHFLVPVQVSAYQGAWRDIPAASRSRLKPGGRNLTLGSSAIAGTRVWDEHAGVRLTLGPLSSEQADSLLPDGTAHQHLAGFAALYFGPDLDCALTLLVAGAKPMVMDREQRPALNWNLGLHRQPTSQQQRIDTYLRQAEIV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9122	-	-

Protein sequence: 340 a.a.

						>T6CP015423 NZ_CP032631:3628865-3629884 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssG]
METAGRQAAVSLSATLRQTPQAFELLQALLVLEREQPQAASLGTGTSPHAEAVRLRGPLTPVFASSQIESLVQEHDQQAV
LTTPVFGLGGPDGPLPYAFQEWLQQRARAKDHAPAEFLDLFQHRLLSLLYRILRKHRIAAGYSVPSASPVQAQLRALTGL
LPQPLQERQALPDAALLARSALFADGRRSLAGFAAIIRQHFLVPVQVSAYQGAWRDIPAASRSRLKPGGRNLTLGSSAIA
GTRVWDEHAGVRLTLGPLSSEQADSLLPDGTAHQHLAGFAALYFGPDLDCALTLLVAGAKPMVMDREQRPALNWNLGLHR
QPTSQQQRIDTYLRQAEIV*					

Nucleotide sequence: 1020 bp

						>T6CP015423 NZ_CP032631:3628865-3629884 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssG]
ATGGAAACCGCAGGCCGGCAGGCCGCTGTCTCTTTAAGCGCGACCCTGCGCCAGACGCCGCAGGCCTTTGAGCTGTTGCA
GGCGCTGCTGGTGCTGGAGCGCGAACAACCTCAGGCCGCGTCGCTGGGCACAGGCACATCGCCGCATGCCGAAGCGGTGC
GGTTGCGCGGCCCGCTGACACCGGTTTTCGCCTCCAGCCAGATTGAAAGCCTGGTTCAGGAGCACGACCAACAGGCGGTG
CTGACCACGCCGGTGTTCGGCCTCGGCGGCCCCGACGGTCCTTTGCCCTACGCCTTTCAGGAATGGCTGCAGCAACGAGC
CCGCGCCAAGGACCACGCACCGGCCGAGTTCCTTGACCTTTTTCAGCATCGGCTGCTCAGCCTGCTCTACCGGATACTGC
GCAAACACCGCATTGCTGCGGGCTACTCAGTACCATCGGCCAGCCCGGTTCAGGCGCAATTGCGCGCACTGACCGGCCTG
CTGCCACAACCACTGCAAGAGCGCCAGGCACTGCCTGATGCCGCCCTTCTGGCCCGCAGCGCATTGTTTGCCGATGGCCG
CCGCTCGCTGGCAGGCTTTGCCGCCATCATCCGCCAACACTTCCTGGTGCCCGTGCAGGTCAGCGCTTATCAGGGCGCAT
GGCGCGACATTCCGGCCGCCAGCCGCAGTCGCCTCAAGCCTGGCGGGCGCAACCTGACACTGGGCAGCAGCGCCATCGCT
GGCACCCGAGTCTGGGATGAACACGCGGGCGTGCGTCTGACCCTTGGCCCACTGAGCAGCGAACAGGCCGACAGCCTGCT
GCCTGACGGCACGGCGCATCAGCACCTGGCCGGGTTTGCCGCGCTGTATTTCGGCCCCGACCTCGACTGCGCGCTGACCT
TGCTGGTGGCAGGCGCAAAACCGATGGTGATGGACCGCGAGCAGCGCCCTGCCCTGAACTGGAACCTTGGCCTGCATCGT
CAGCCCACGTCACAACAGCAGCGCATCGACACTTACCTGCGCCAGGCGGAGATTGTCTGA					