Detailed information of component protein
Summary
| Component ID | T6CP015415 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01219  (Type i4a) |
| Strain | Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 |
| Replicon | chromosome |
| Sequence | Protein sequence (257 a.a.); Nucleotide sequence (771 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | CN228_RS17565 |
| Coordinate (Strand) | 3621162..3621932 (-) |
| NCBI ID | WP_017684346.1 |
| RefSeq | NZ_CP032631 |
| Uniprot ID | A0A2V0QXE7_PSESF |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 1.9e-30 | 74..226 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-209 | MPEGNAVARSRAVQEAPLSHAFRQAWQEWLSAWRELDKSVEDTGKLVDTVVELSTRITRRLWRTAFASVGDSATLQMKAMVYAFVALVDETLLFADWSGQSAWQEKPLESRLYSSRQAGQRVPLEIKRLLDEQAPTSRDLGNVYLLCLILGFQGHLRGERGRALHEKWRNALYTFTRQREPDFTNVSQQLTEPASAAARTLALRQSMPD |
| Transmembrane helix | 210-232 | GMRLALIILLATVLLIGVGHLFW |
| Inside | 233-256 | RDIGQRLEPAFNSGSISSTTEQPQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9910 | - | - |
Protein sequence: 257 a.a.
>T6CP015415 NZ_CP032631:c3621932-3621162 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssL]
MPEGNAVARSRAVQEAPLSHAFRQAWQEWLSAWRELDKSVEDTGKLVDTVVELSTRITRRLWRTAFASVGDSATLQMKAM
VYAFVALVDETLLFADWSGQSAWQEKPLESRLYSSRQAGQRVPLEIKRLLDEQAPTSRDLGNVYLLCLILGFQGHLRGER
GRALHEKWRNALYTFTRQREPDFTNVSQQLTEPASAAARTLALRQSMPDGMRLALIILLATVLLIGVGHLFWRDIGQRLE
PAFNSGSISSTTEQPQ*
MPEGNAVARSRAVQEAPLSHAFRQAWQEWLSAWRELDKSVEDTGKLVDTVVELSTRITRRLWRTAFASVGDSATLQMKAM
VYAFVALVDETLLFADWSGQSAWQEKPLESRLYSSRQAGQRVPLEIKRLLDEQAPTSRDLGNVYLLCLILGFQGHLRGER
GRALHEKWRNALYTFTRQREPDFTNVSQQLTEPASAAARTLALRQSMPDGMRLALIILLATVLLIGVGHLFWRDIGQRLE
PAFNSGSISSTTEQPQ*
Nucleotide sequence: 771 bp
>T6CP015415 NZ_CP032631:c3621932-3621162 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssL]
ATGCCTGAAGGGAATGCAGTGGCGCGTTCGCGCGCAGTGCAGGAAGCACCGCTGAGCCATGCGTTTCGTCAAGCCTGGCA
GGAGTGGCTGAGCGCCTGGCGCGAGCTGGACAAAAGTGTCGAAGACACCGGCAAGCTGGTGGACACAGTGGTCGAGCTGT
CTACCCGGATCACCCGACGACTGTGGCGCACTGCGTTCGCCAGCGTCGGGGACTCGGCGACTCTGCAAATGAAAGCGATG
GTGTACGCTTTCGTTGCGCTGGTGGATGAGACCCTGCTGTTCGCCGACTGGTCCGGGCAGAGCGCCTGGCAGGAAAAACC
GCTGGAATCGCGGCTGTACTCCAGCCGCCAGGCCGGCCAGCGCGTGCCGCTTGAAATCAAGCGCCTGCTCGACGAGCAGG
CGCCCACCAGCCGCGATCTGGGGAATGTTTACTTGCTGTGCCTGATCCTTGGTTTCCAGGGGCACTTGCGCGGCGAACGG
GGCAGGGCGCTGCACGAAAAATGGCGCAACGCGCTGTACACCTTCACCCGTCAGCGCGAACCTGACTTTACCAATGTCAG
CCAGCAACTGACCGAACCCGCCAGCGCGGCAGCGCGCACACTTGCGCTGCGCCAATCGATGCCTGACGGCATGCGCCTTG
CCCTGATCATCCTGCTGGCCACGGTGTTGCTGATCGGCGTCGGTCATCTGTTCTGGCGGGATATCGGCCAACGGCTTGAG
CCTGCTTTCAATTCCGGCAGCATCTCTTCAACTACGGAGCAGCCGCAATGA
ATGCCTGAAGGGAATGCAGTGGCGCGTTCGCGCGCAGTGCAGGAAGCACCGCTGAGCCATGCGTTTCGTCAAGCCTGGCA
GGAGTGGCTGAGCGCCTGGCGCGAGCTGGACAAAAGTGTCGAAGACACCGGCAAGCTGGTGGACACAGTGGTCGAGCTGT
CTACCCGGATCACCCGACGACTGTGGCGCACTGCGTTCGCCAGCGTCGGGGACTCGGCGACTCTGCAAATGAAAGCGATG
GTGTACGCTTTCGTTGCGCTGGTGGATGAGACCCTGCTGTTCGCCGACTGGTCCGGGCAGAGCGCCTGGCAGGAAAAACC
GCTGGAATCGCGGCTGTACTCCAGCCGCCAGGCCGGCCAGCGCGTGCCGCTTGAAATCAAGCGCCTGCTCGACGAGCAGG
CGCCCACCAGCCGCGATCTGGGGAATGTTTACTTGCTGTGCCTGATCCTTGGTTTCCAGGGGCACTTGCGCGGCGAACGG
GGCAGGGCGCTGCACGAAAAATGGCGCAACGCGCTGTACACCTTCACCCGTCAGCGCGAACCTGACTTTACCAATGTCAG
CCAGCAACTGACCGAACCCGCCAGCGCGGCAGCGCGCACACTTGCGCTGCGCCAATCGATGCCTGACGGCATGCGCCTTG
CCCTGATCATCCTGCTGGCCACGGTGTTGCTGATCGGCGTCGGTCATCTGTTCTGGCGGGATATCGGCCAACGGCTTGAG
CCTGCTTTCAATTCCGGCAGCATCTCTTCAACTACGGAGCAGCCGCAATGA