SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP015413
Component protein type	TssA
T6SS ID (Type)	T6SS01219 (Type i4a)
Strain	Pseudomonas syringae pv. actinidiae str. Shaanxi_M228
Replicon	chromosome
Sequence	Protein sequence (367 a.a.); Nucleotide sequence (1101 bp)
Reference	[1] Wang N, Han N, Tian R, et al. Role of the Type VI Secretion System in the Pathogenicity of Pseudomonas syringae pv. actinidiae, the Causative Agent of Kiwifruit Bacterial Canker. Front Microbiol. 2021 Feb 19;12:627785. doi: 10.3389/fmicb.2021.627785. eCollection 2021. PMID: 33679650

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	CN228_RS17555
Coordinate (Strand)	3616254..3617354 (-)
NCBI ID	WP_017684347.1
RefSeq	NZ_CP032631
Uniprot ID	A0A2V0R546_PSESF
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	4.4e-34	12..134

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-366	MNQLSARVSSLLEPVSAASACGPSLRYDPVYDHLRQLRVQEDDSLPRGVWQTELKRADWNQVEALACSLLAERSKDLMVAAWLGEAWLHKDALSGIVDALSLVEQLCERFPEALHPQPVDGDQSWRAAPLEWLSRRYAELLVTQLPLFTADEVDFTGYSLSDWRQMTRKQVLANDSKAAKVSAETAINDQKKLNLRVRNTPLSWWLNHRDAIQIALERLERLENWAERHLEDQAPTFGPLQSSLQALTALFNEFIAMHPPQVLVPVLSEALPMETSDMPTDPVPDGNMGREQLTARAFVEPRNREEAYRHLLCIADYLARTEPHSPVPYLIKKGVEWGNQPLKELLSELISSDSDARRVWTLLGVL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.5633	-	-

Protein sequence: 367 a.a.

						>T6CP015413 NZ_CP032631:c3617354-3616254 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssA]
MNQLSARVSSLLEPVSAASACGPSLRYDPVYDHLRQLRVQEDDSLPRGVWQTELKRADWNQVEALACSLLAERSKDLMVA
AWLGEAWLHKDALSGIVDALSLVEQLCERFPEALHPQPVDGDQSWRAAPLEWLSRRYAELLVTQLPLFTADEVDFTGYSL
SDWRQMTRKQVLANDSKAAKVSAETAINDQKKLNLRVRNTPLSWWLNHRDAIQIALERLERLENWAERHLEDQAPTFGPL
QSSLQALTALFNEFIAMHPPQVLVPVLSEALPMETSDMPTDPVPDGNMGREQLTARAFVEPRNREEAYRHLLCIADYLAR
TEPHSPVPYLIKKGVEWGNQPLKELLSELISSDSDARRVWTLLGVL*					

Nucleotide sequence: 1101 bp

						>T6CP015413 NZ_CP032631:c3617354-3616254 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssA]
ATGAACCAGCTATCCGCCCGGGTCAGTTCGCTGCTTGAGCCTGTCAGTGCTGCGTCTGCCTGCGGCCCGAGCCTTCGCTA
CGATCCGGTTTACGATCATCTGCGCCAGCTGCGGGTTCAGGAAGACGACAGCCTGCCGCGTGGTGTCTGGCAAACAGAGC
TGAAACGGGCGGACTGGAACCAGGTCGAAGCGCTGGCCTGTTCGCTGCTGGCTGAGCGAAGCAAGGACCTGATGGTGGCC
GCGTGGCTGGGCGAAGCCTGGCTGCACAAGGATGCGCTGTCCGGCATTGTCGATGCGCTGAGCCTGGTCGAGCAATTGTG
CGAGCGGTTCCCGGAGGCGCTGCATCCCCAGCCTGTCGATGGCGACCAGTCCTGGCGAGCAGCGCCACTGGAGTGGCTGT
CGCGCCGTTATGCAGAACTGCTGGTCACCCAGTTGCCGTTGTTTACAGCCGACGAGGTCGATTTTACCGGGTACAGCCTC
AGTGACTGGCGGCAGATGACCCGCAAGCAGGTACTGGCCAATGACAGTAAGGCCGCCAAGGTGTCGGCGGAAACCGCGAT
CAACGATCAGAAGAAACTCAACCTGCGGGTGCGCAACACGCCGTTGAGCTGGTGGCTGAACCACCGGGATGCCATTCAGA
TAGCGCTCGAGAGGCTGGAACGTCTGGAAAACTGGGCTGAGCGACACCTTGAGGATCAGGCACCCACCTTCGGGCCGTTA
CAGAGCAGCCTTCAGGCACTGACGGCGCTGTTCAATGAATTTATTGCCATGCACCCGCCGCAGGTCCTGGTGCCTGTGCT
GTCTGAGGCTTTGCCTATGGAAACGTCTGATATGCCAACTGACCCAGTGCCTGACGGCAATATGGGCCGCGAGCAACTGA
CGGCACGTGCCTTCGTCGAGCCGCGTAACCGCGAGGAGGCGTACCGCCATTTGTTGTGCATCGCTGATTATCTGGCCAGA
ACCGAGCCCCACAGTCCAGTGCCTTATCTGATCAAAAAGGGCGTGGAGTGGGGCAACCAGCCGCTGAAGGAGTTGCTGTC
CGAGCTGATTTCATCCGACTCTGATGCGCGTCGGGTCTGGACGTTGCTGGGCGTGCTGTAA					