Detailed information of component protein
Summary
| Component ID | T6CP015418 |
| Component protein type | TssB |
| T6SS ID (Type) | T6SS01219  (Type i4a) |
| Strain | Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 |
| Replicon | chromosome |
| Sequence | Protein sequence (184 a.a.); Nucleotide sequence (552 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | CN228_RS17580 |
| Coordinate (Strand) | 3624009..3624560 (+) |
| NCBI ID | WP_017684344.1 |
| RefSeq | NZ_CP032631 |
| Uniprot ID | A0A0K8LX03_PSESF, A0A0Q0DWQ7_PSESX, A0A0Q0GHZ0_9PSED, A0A261WDA3_9PSED |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 1.3e-56 | 10..161 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-183 | MAESTQHKLDRVRPPRVQITYDVEIGNAIEKKELPLVVGILADLSGKPDAPLPKLIDRRFVEIDRDNFDEVLASIAPRTAIQVKNTLSGDDSSLNVELRFSHIDDFDPINLVKQVTPLRRLLEARQRLRDLLTKLDGNDDLDKLLQEVVANTEGLQEIKSAAPQDAPAAPATVEGEPPAEPQA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9983 | - | - |
Protein sequence: 184 a.a.
>T6CP015418 NZ_CP032631:3624009-3624560 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssB]
MAESTQHKLDRVRPPRVQITYDVEIGNAIEKKELPLVVGILADLSGKPDAPLPKLIDRRFVEIDRDNFDEVLASIAPRTA
IQVKNTLSGDDSSLNVELRFSHIDDFDPINLVKQVTPLRRLLEARQRLRDLLTKLDGNDDLDKLLQEVVANTEGLQEIKS
AAPQDAPAAPATVEGEPPAEPQA*
MAESTQHKLDRVRPPRVQITYDVEIGNAIEKKELPLVVGILADLSGKPDAPLPKLIDRRFVEIDRDNFDEVLASIAPRTA
IQVKNTLSGDDSSLNVELRFSHIDDFDPINLVKQVTPLRRLLEARQRLRDLLTKLDGNDDLDKLLQEVVANTEGLQEIKS
AAPQDAPAAPATVEGEPPAEPQA*
Nucleotide sequence: 552 bp
>T6CP015418 NZ_CP032631:3624009-3624560 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssB]
ATGGCCGAAAGTACTCAGCACAAGCTAGACCGGGTTCGCCCGCCGCGTGTTCAGATCACTTACGACGTTGAAATCGGTAA
TGCAATCGAGAAAAAGGAATTGCCTCTCGTGGTTGGCATACTTGCCGATCTGTCCGGCAAGCCCGATGCGCCCCTCCCCA
AACTGATCGACCGACGCTTTGTCGAAATCGATCGGGACAACTTCGACGAAGTCCTCGCCTCCATCGCTCCGCGAACGGCC
ATTCAGGTCAAGAACACCCTCAGTGGTGACGATAGCTCGCTTAACGTCGAATTGCGCTTCAGCCACATCGACGATTTTGA
CCCGATCAATCTGGTCAAGCAGGTCACGCCGCTGCGTCGCCTGCTCGAAGCCCGTCAACGCCTGCGCGATCTGCTGACCA
AGCTTGATGGCAACGACGACCTGGACAAGCTGCTCCAGGAAGTGGTCGCCAACACCGAAGGCCTGCAGGAAATCAAGTCA
GCGGCCCCGCAAGACGCACCTGCTGCGCCAGCGACGGTCGAGGGCGAACCGCCTGCCGAGCCGCAAGCCTGA
ATGGCCGAAAGTACTCAGCACAAGCTAGACCGGGTTCGCCCGCCGCGTGTTCAGATCACTTACGACGTTGAAATCGGTAA
TGCAATCGAGAAAAAGGAATTGCCTCTCGTGGTTGGCATACTTGCCGATCTGTCCGGCAAGCCCGATGCGCCCCTCCCCA
AACTGATCGACCGACGCTTTGTCGAAATCGATCGGGACAACTTCGACGAAGTCCTCGCCTCCATCGCTCCGCGAACGGCC
ATTCAGGTCAAGAACACCCTCAGTGGTGACGATAGCTCGCTTAACGTCGAATTGCGCTTCAGCCACATCGACGATTTTGA
CCCGATCAATCTGGTCAAGCAGGTCACGCCGCTGCGTCGCCTGCTCGAAGCCCGTCAACGCCTGCGCGATCTGCTGACCA
AGCTTGATGGCAACGACGACCTGGACAAGCTGCTCCAGGAAGTGGTCGCCAACACCGAAGGCCTGCAGGAAATCAAGTCA
GCGGCCCCGCAAGACGCACCTGCTGCGCCAGCGACGGTCGAGGGCGAACCGCCTGCCGAGCCGCAAGCCTGA