Detailed information of component protein
Summary
| Component ID | T6CP015420 |
| Component protein type | TssD |
| T6SS ID (Type) | T6SS01219  (Type i4a) |
| Strain | Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 |
| Replicon | chromosome |
| Sequence | Protein sequence (166 a.a.); Nucleotide sequence (498 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | CN228_RS17590 |
| Coordinate (Strand) | 3626159..3626656 (+) |
| NCBI ID | WP_003382818.1 |
| RefSeq | NZ_CP032631 |
| Uniprot ID | A0A0K8LVH9_PSESF, A0A0Q0E7D9_PSESX, A0A0P9M4H6_9PSED, A0A2S3VBM9_PSESX, A0A261WDE1_9PSED, A0A3M3S092_9PSED |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 4.6e-30 | 10..138 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-165 | MDAIILDLGDEIKGDSLLEGYKDKIEIMSYSHNVAMQVTNDVSNSERTSGKPHIGEFTLTKFVDSSTPTLNSYCCAGKPIPKATIVIGRNAAEGSGQLMAFITYTLDNVVLSNVSVSGGTGGKPVETLSLNFTKIKWELTAQKDDGTKEGVAGTSWDMAANKKAS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9916 | - | - |
Protein sequence: 166 a.a.
>T6CP015420 NZ_CP032631:3626159-3626656 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssD]
MDAIILDLGDEIKGDSLLEGYKDKIEIMSYSHNVAMQVTNDVSNSERTSGKPHIGEFTLTKFVDSSTPTLNSYCCAGKPI
PKATIVIGRNAAEGSGQLMAFITYTLDNVVLSNVSVSGGTGGKPVETLSLNFTKIKWELTAQKDDGTKEGVAGTSWDMAA
NKKAS*
MDAIILDLGDEIKGDSLLEGYKDKIEIMSYSHNVAMQVTNDVSNSERTSGKPHIGEFTLTKFVDSSTPTLNSYCCAGKPI
PKATIVIGRNAAEGSGQLMAFITYTLDNVVLSNVSVSGGTGGKPVETLSLNFTKIKWELTAQKDDGTKEGVAGTSWDMAA
NKKAS*
Nucleotide sequence: 498 bp
>T6CP015420 NZ_CP032631:3626159-3626656 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssD]
ATGGATGCGATCATTCTCGATCTGGGCGACGAGATCAAAGGCGACAGTTTGCTGGAAGGTTACAAGGACAAGATCGAAAT
CATGTCCTACAGCCACAACGTGGCCATGCAGGTTACAAACGATGTCAGCAACTCCGAGCGGACTTCGGGCAAGCCACACA
TCGGCGAGTTCACCCTGACCAAGTTCGTCGACAGCTCCACCCCGACCCTCAACAGCTATTGCTGCGCCGGCAAGCCGATT
CCGAAAGCGACCATTGTCATCGGGCGTAACGCGGCAGAGGGCAGCGGCCAACTGATGGCGTTCATCACCTACACGCTGGA
TAACGTGGTGCTGTCAAACGTCAGTGTCAGTGGCGGTACCGGTGGCAAACCGGTCGAAACCCTGTCGCTGAACTTCACCA
AGATCAAATGGGAGCTGACCGCCCAGAAAGACGACGGCACCAAGGAAGGCGTGGCCGGCACCAGCTGGGACATGGCCGCC
AACAAGAAAGCCAGTTAA
ATGGATGCGATCATTCTCGATCTGGGCGACGAGATCAAAGGCGACAGTTTGCTGGAAGGTTACAAGGACAAGATCGAAAT
CATGTCCTACAGCCACAACGTGGCCATGCAGGTTACAAACGATGTCAGCAACTCCGAGCGGACTTCGGGCAAGCCACACA
TCGGCGAGTTCACCCTGACCAAGTTCGTCGACAGCTCCACCCCGACCCTCAACAGCTATTGCTGCGCCGGCAAGCCGATT
CCGAAAGCGACCATTGTCATCGGGCGTAACGCGGCAGAGGGCAGCGGCCAACTGATGGCGTTCATCACCTACACGCTGGA
TAACGTGGTGCTGTCAAACGTCAGTGTCAGTGGCGGTACCGGTGGCAAACCGGTCGAAACCCTGTCGCTGAACTTCACCA
AGATCAAATGGGAGCTGACCGCCCAGAAAGACGACGGCACCAAGGAAGGCGTGGCCGGCACCAGCTGGGACATGGCCGCC
AACAAGAAAGCCAGTTAA