Detailed information of component protein
Summary
| Component ID | T6CP015421 |
| Component protein type | TssE |
| T6SS ID (Type) | T6SS01219  (Type i4a) |
| Strain | Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 |
| Replicon | chromosome |
| Sequence | Protein sequence (150 a.a.); Nucleotide sequence (450 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | CN228_RS17595 |
| Coordinate (Strand) | 3626672..3627121 (+) |
| NCBI ID | WP_003382819.1 |
| RefSeq | NZ_CP032631 |
| Uniprot ID | A0A0K8LVH5_PSESF, A0A0N8TL14_PSESX, A0A261WDJ5_9PSED |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| GPW_gp25 | PF04965.16 | 4.1e-19 | 31..124 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-149 | MSGTGIRPLLFERLAFSEDESQRPTFLDREALADSVRSELMRLLNTRRAAANMTLPPTLLDYGIADWSSLQASNSDDRRRMTREIRAAITQFEPRLQLSDVDVQSVQGHPQRLSIRLDGILRNDRQQWPVAFILHTSEAGMEVSHERFD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9763 | - | - |
Protein sequence: 150 a.a.
>T6CP015421 NZ_CP032631:3626672-3627121 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssE]
MSGTGIRPLLFERLAFSEDESQRPTFLDREALADSVRSELMRLLNTRRAAANMTLPPTLLDYGIADWSSLQASNSDDRRR
MTREIRAAITQFEPRLQLSDVDVQSVQGHPQRLSIRLDGILRNDRQQWPVAFILHTSEAGMEVSHERFD*
MSGTGIRPLLFERLAFSEDESQRPTFLDREALADSVRSELMRLLNTRRAAANMTLPPTLLDYGIADWSSLQASNSDDRRR
MTREIRAAITQFEPRLQLSDVDVQSVQGHPQRLSIRLDGILRNDRQQWPVAFILHTSEAGMEVSHERFD*
Nucleotide sequence: 450 bp
>T6CP015421 NZ_CP032631:3626672-3627121 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssE]
ATGTCCGGCACAGGGATCCGCCCGCTGTTGTTCGAGCGCCTGGCGTTCAGCGAGGACGAGTCGCAGCGCCCGACGTTTCT
CGACCGGGAGGCGCTGGCCGACTCAGTGCGCAGCGAACTGATGCGCCTGCTCAATACCCGGCGGGCGGCGGCCAACATGA
CGTTACCCCCGACCCTGCTGGATTACGGAATCGCCGACTGGAGCAGCTTGCAGGCCAGCAACAGTGACGATCGGCGGCGC
ATGACACGTGAAATACGCGCCGCCATCACTCAGTTCGAGCCACGCCTGCAACTGTCCGATGTCGATGTTCAGTCGGTGCA
GGGGCATCCGCAGCGATTGAGCATCCGCCTCGACGGAATCCTGCGCAACGACCGCCAGCAATGGCCCGTGGCATTTATCC
TGCACACATCCGAAGCAGGCATGGAGGTGAGCCATGAGCGATTCGATTGA
ATGTCCGGCACAGGGATCCGCCCGCTGTTGTTCGAGCGCCTGGCGTTCAGCGAGGACGAGTCGCAGCGCCCGACGTTTCT
CGACCGGGAGGCGCTGGCCGACTCAGTGCGCAGCGAACTGATGCGCCTGCTCAATACCCGGCGGGCGGCGGCCAACATGA
CGTTACCCCCGACCCTGCTGGATTACGGAATCGCCGACTGGAGCAGCTTGCAGGCCAGCAACAGTGACGATCGGCGGCGC
ATGACACGTGAAATACGCGCCGCCATCACTCAGTTCGAGCCACGCCTGCAACTGTCCGATGTCGATGTTCAGTCGGTGCA
GGGGCATCCGCAGCGATTGAGCATCCGCCTCGACGGAATCCTGCGCAACGACCGCCAGCAATGGCCCGTGGCATTTATCC
TGCACACATCCGAAGCAGGCATGGAGGTGAGCCATGAGCGATTCGATTGA