Detailed information of component protein


Summary

Component ID T6CP015422
Component protein type TssF
T6SS ID (Type) T6SS01219 experimental (Type i4a)
Strain Pseudomonas syringae pv. actinidiae str. Shaanxi_M228
Replicon chromosome
Sequence Protein sequence (599 a.a.); Nucleotide sequence (1797 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) CN228_RS17600
Coordinate (Strand) 3627105..3628901 (+)
NCBI ID WP_024533274.1
RefSeq NZ_CP032631
Uniprot ID UPI000464216E
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 1e-180 6..594
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-598 MSDSIDPQLLDYYQRELTWLRHAGGVFTNRYPKVARRLELSPGESQDPHVERLLEGFALLAARLQRRLDDDYAEFSDALLEQLYPLSLRPLPSSTVVRFEPDPAKGNLATGYDLPRDTPLFVTTTQGETIHFRTCASVTLWPVQISEALLLSAEEAQALTDVAQARSALRLTLDCLGEKTTWSALTLQHLRLHLNASPVVNAVLYDLLGAHTIKVLSGAPGSLPAAEPTLPAIVGFADDEALLPEEDGVHPGRRLIAEYFAFPEKFNFFDIPVHAAHVEGQTLYLYLVFDRAPQARLHLQASDIALGCTPAINLFPRTSEPVRPDGTQSEYRLVADSHRENSVEIHSIRGMRASSPRGVSRVSAYYGSQHSQGDNPLYWHARRINGLTPNRLGSDLLISLVDTHFVPVRDTPDYTLTAELLCTNRYLAQSLIAGTALSFERPGPVARASIINPPSSQSAPHLNGESRWRLVSQLTLNHLSLVEGPKALEALKEILGLHNLRDEASARRQIDGLHRLSCERVVAQVGEDAWRGWRNGLEVSLELDPEHFVGNSAVLFSAVLAQFFSLYATANRFVRTVLISADKDVMVWKPQAGRPLSL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9943 - -


  Protein sequence: 599 a.a.    

>T6CP015422 NZ_CP032631:3627105-3628901 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssF]
MSDSIDPQLLDYYQRELTWLRHAGGVFTNRYPKVARRLELSPGESQDPHVERLLEGFALLAARLQRRLDDDYAEFSDALL
EQLYPLSLRPLPSSTVVRFEPDPAKGNLATGYDLPRDTPLFVTTTQGETIHFRTCASVTLWPVQISEALLLSAEEAQALT
DVAQARSALRLTLDCLGEKTTWSALTLQHLRLHLNASPVVNAVLYDLLGAHTIKVLSGAPGSLPAAEPTLPAIVGFADDE
ALLPEEDGVHPGRRLIAEYFAFPEKFNFFDIPVHAAHVEGQTLYLYLVFDRAPQARLHLQASDIALGCTPAINLFPRTSE
PVRPDGTQSEYRLVADSHRENSVEIHSIRGMRASSPRGVSRVSAYYGSQHSQGDNPLYWHARRINGLTPNRLGSDLLISL
VDTHFVPVRDTPDYTLTAELLCTNRYLAQSLIAGTALSFERPGPVARASIINPPSSQSAPHLNGESRWRLVSQLTLNHLS
LVEGPKALEALKEILGLHNLRDEASARRQIDGLHRLSCERVVAQVGEDAWRGWRNGLEVSLELDPEHFVGNSAVLFSAVL
AQFFSLYATANRFVRTVLISADKDVMVWKPQAGRPLSL*

  Nucleotide sequence: 1797 bp    

>T6CP015422 NZ_CP032631:3627105-3628901 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssF]
ATGAGCGATTCGATTGACCCGCAACTGCTCGACTATTATCAGCGCGAACTCACCTGGCTGCGGCATGCAGGCGGTGTGTT
TACCAATCGCTACCCCAAAGTCGCCAGGCGTCTGGAGCTGTCTCCCGGCGAAAGCCAGGACCCGCATGTGGAGCGCCTGC
TGGAGGGCTTTGCCCTGCTCGCAGCACGCCTGCAACGGCGGCTTGATGACGACTACGCAGAATTCAGTGACGCCCTGCTC
GAACAGCTTTACCCCTTGTCGCTAAGGCCATTGCCTTCGTCCACAGTGGTGCGTTTCGAGCCTGATCCGGCCAAAGGCAA
CCTGGCTACCGGCTACGACCTGCCGCGCGACACACCGCTTTTCGTGACCACCACTCAGGGCGAAACCATCCACTTTCGCA
CCTGTGCCAGTGTCACCCTGTGGCCTGTGCAGATCAGCGAAGCGCTGCTGCTCAGCGCTGAAGAAGCCCAGGCGCTGACC
GACGTGGCACAGGCGCGTTCGGCATTGCGCCTGACCCTCGATTGCCTGGGCGAAAAGACCACCTGGTCAGCGCTGACGCT
ACAGCACCTGCGCCTGCACCTGAACGCCTCACCGGTGGTCAACGCCGTGCTTTATGACCTGCTCGGCGCGCACACCATCA
AAGTGCTAAGTGGCGCGCCGGGCAGCCTTCCCGCTGCCGAGCCGACGCTGCCCGCCATCGTCGGTTTTGCCGATGACGAA
GCCCTGCTCCCCGAAGAAGATGGCGTACACCCCGGCAGGCGTTTGATTGCCGAATATTTCGCCTTCCCGGAAAAATTCAA
CTTCTTCGACATCCCCGTGCACGCCGCGCACGTCGAAGGTCAGACGCTGTACCTGTACTTGGTCTTTGACCGCGCCCCGC
AGGCCCGCCTGCACTTGCAGGCCAGTGATATTGCTCTGGGCTGCACGCCGGCGATCAACCTGTTCCCGCGCACCTCGGAG
CCGGTCAGGCCAGACGGCACACAGAGCGAATACCGCTTGGTGGCGGACAGTCACCGCGAGAACAGTGTGGAAATCCACAG
CATCCGCGGCATGCGGGCCAGCTCGCCAAGAGGCGTCAGCCGTGTATCCGCTTATTACGGCAGCCAACACTCACAAGGGG
ACAACCCGCTGTACTGGCATGCGCGGCGCATCAACGGTCTGACGCCCAATCGTCTGGGCAGCGATCTGCTGATCAGTCTG
GTGGACACTCATTTCGTCCCTGTGCGCGACACCCCGGACTACACCCTGACCGCCGAACTGCTGTGCACCAATCGGTATCT
GGCACAGAGCCTGATCGCCGGAACCGCCCTGAGTTTCGAGCGCCCCGGTCCGGTCGCCCGCGCCAGCATCATCAACCCGC
CCAGCTCGCAAAGCGCACCGCATTTGAACGGTGAATCGCGCTGGCGTCTGGTGTCGCAACTGACCCTCAACCACTTGTCA
CTGGTGGAAGGCCCCAAGGCACTTGAGGCGCTCAAGGAAATTCTTGGCCTGCACAACCTGCGCGACGAAGCCAGCGCCCG
TCGGCAGATCGACGGCTTGCACAGGTTGAGCTGCGAGCGGGTCGTCGCGCAGGTCGGTGAAGACGCCTGGCGGGGCTGGC
GCAACGGGCTGGAAGTCAGCCTGGAACTGGACCCCGAGCATTTCGTCGGCAACAGCGCGGTACTTTTCTCCGCCGTGTTG
GCGCAGTTTTTCTCGTTGTACGCCACCGCCAACCGCTTCGTACGCACGGTGCTGATCAGCGCCGACAAGGACGTCATGGT
ATGGAAACCGCAGGCCGGCAGGCCGCTGTCTCTTTAA