Detailed information of component protein


Summary

Component ID T6CP015425
Component protein type TssI
T6SS ID (Type) T6SS01219 experimental (Type i4a)
Strain Pseudomonas syringae pv. actinidiae str. Shaanxi_M228
Replicon chromosome
Sequence Protein sequence (669 a.a.); Nucleotide sequence (2007 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) CN228_RS17635
Coordinate (Strand) 3636567..3638573 (+)
NCBI ID WP_017704523.1
RefSeq NZ_CP032631
Uniprot ID UPI00034601B4
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 8.9e-81 47..322
Phage_base_V PF04717.14 3e-16 368..435
Gp5_C PF06715.14 1.7e-06 560..583
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-668 MPRPTDTTTTLSLTGTALTALYPESVIGEESLNSLGQQVLHGQCDAPLTLTTAIASHVTLTLHNDALQRPLDALVAEIRQLPADASADRYQVLLRPWLWWLTLASNNRVFQNLTTSDIVTSIFTAHGFSDFRLSLTSSYTPREYCVQYGETDFAFVSRLLEEEGIFWFFTHDSGKHTLVLADSNDAFVPIPNGPAVKYLGQQMGDRELHGIRSGQLVLQAVSGVYSATDYAFATPATSLYSKAEAKAGPLSVYEHPGGYVAKAQGDALTKQRVDGLRSQEQRFIGESDCRWLVPGHFFTLGGHDDAALNIDWVVTAVSHEASHERYRNRFEAIPRATAYRPERKTPKPRMHTQTAIVVGKAGEEIWTDEYGRIKIQFPWDRDGKNDESSSCWVRVVLPWSGKGFGMQFVPRIGQEVIVTFIDGDPDRPLVSGCVYNGDNALPYALPANQTQSGIKTNSSKGGGGFNELRFEDKKDAEEVFLQAQKDFNTNVLNDTTVKVGHDETLTVQNARTRTVKEGDETVTLEKGKRSVAIQTGSDTLDVKDTRSVTVGGDQTHSTGGNYTDKVTGNYSLTVDGNLTIKVSGTLTLESGGSFSIKSGADLATQASTAISHKAGTSLANQAGTTLENKAGTTMTNDAGISLINKASASQTVDGGGLLTLKGGLVKVN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8246 - -


  Protein sequence: 669 a.a.    

>T6CP015425 NZ_CP032631:3636567-3638573 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssI]
MPRPTDTTTTLSLTGTALTALYPESVIGEESLNSLGQQVLHGQCDAPLTLTTAIASHVTLTLHNDALQRPLDALVAEIRQ
LPADASADRYQVLLRPWLWWLTLASNNRVFQNLTTSDIVTSIFTAHGFSDFRLSLTSSYTPREYCVQYGETDFAFVSRLL
EEEGIFWFFTHDSGKHTLVLADSNDAFVPIPNGPAVKYLGQQMGDRELHGIRSGQLVLQAVSGVYSATDYAFATPATSLY
SKAEAKAGPLSVYEHPGGYVAKAQGDALTKQRVDGLRSQEQRFIGESDCRWLVPGHFFTLGGHDDAALNIDWVVTAVSHE
ASHERYRNRFEAIPRATAYRPERKTPKPRMHTQTAIVVGKAGEEIWTDEYGRIKIQFPWDRDGKNDESSSCWVRVVLPWS
GKGFGMQFVPRIGQEVIVTFIDGDPDRPLVSGCVYNGDNALPYALPANQTQSGIKTNSSKGGGGFNELRFEDKKDAEEVF
LQAQKDFNTNVLNDTTVKVGHDETLTVQNARTRTVKEGDETVTLEKGKRSVAIQTGSDTLDVKDTRSVTVGGDQTHSTGG
NYTDKVTGNYSLTVDGNLTIKVSGTLTLESGGSFSIKSGADLATQASTAISHKAGTSLANQAGTTLENKAGTTMTNDAGI
SLINKASASQTVDGGGLLTLKGGLVKVN*

  Nucleotide sequence: 2007 bp    

>T6CP015425 NZ_CP032631:3636567-3638573 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228] [TssI]
ATGCCCCGTCCCACCGACACCACAACCACCCTCTCCCTGACCGGCACAGCCCTCACCGCGCTGTACCCGGAGTCGGTCAT
TGGCGAGGAATCACTCAACAGCCTTGGCCAGCAGGTACTGCATGGCCAGTGCGATGCGCCGTTGACGCTGACCACCGCCA
TTGCTTCGCACGTCACCCTGACCCTGCACAACGACGCCCTGCAACGCCCGCTGGATGCGCTGGTTGCAGAGATTCGTCAG
CTGCCGGCCGACGCCTCGGCAGACCGTTATCAGGTGCTGTTGCGGCCGTGGTTATGGTGGTTGACGTTGGCGAGCAACAA
CCGGGTGTTCCAGAACCTGACGACGTCGGACATTGTCACGTCGATCTTCACCGCTCACGGTTTCAGTGATTTCAGGCTGT
CGCTGACTAGCAGCTACACGCCTCGCGAGTATTGCGTGCAGTACGGCGAGACGGATTTCGCGTTTGTCTCGCGCTTGCTG
GAGGAAGAAGGGATTTTCTGGTTTTTCACCCATGACTCAGGCAAGCACACGCTGGTGCTGGCGGACAGCAACGATGCCTT
TGTGCCGATCCCCAATGGCCCGGCGGTAAAGTACCTGGGCCAGCAAATGGGTGATCGGGAGTTGCACGGCATTCGTTCCG
GACAACTCGTGTTGCAGGCGGTTTCCGGGGTCTATTCGGCGACCGATTACGCGTTTGCCACGCCGGCAACGTCGCTGTAC
AGCAAGGCCGAAGCCAAGGCCGGGCCGTTGTCGGTGTACGAGCATCCGGGCGGTTATGTCGCCAAGGCACAAGGCGATGC
ATTGACCAAGCAACGGGTCGATGGCCTGCGCAGTCAGGAGCAACGTTTCATTGGCGAAAGCGACTGTCGCTGGCTGGTGC
CGGGGCACTTTTTCACCCTCGGCGGGCATGATGATGCGGCGCTGAACATCGATTGGGTGGTGACGGCGGTCAGTCACGAA
GCCAGCCATGAACGCTATCGCAATCGTTTTGAAGCCATTCCCAGGGCCACGGCTTATCGCCCGGAACGCAAAACGCCCAA
GCCGCGCATGCATACCCAGACGGCCATCGTGGTCGGCAAGGCGGGCGAGGAAATCTGGACCGATGAATATGGCCGCATCA
AGATCCAGTTCCCCTGGGATCGCGACGGCAAGAATGATGAAAGCAGTTCCTGCTGGGTGCGTGTGGTGCTGCCCTGGAGC
GGCAAGGGCTTCGGCATGCAGTTCGTGCCGCGCATCGGCCAGGAAGTGATCGTGACCTTTATCGACGGCGACCCGGACCG
GCCTCTGGTCAGCGGCTGCGTGTACAACGGCGACAACGCCCTGCCCTATGCGCTGCCCGCCAACCAGACGCAATCGGGCA
TCAAGACCAACTCTTCCAAAGGCGGCGGCGGGTTCAACGAGCTGCGTTTCGAGGACAAGAAAGACGCTGAAGAGGTCTTT
CTGCAAGCGCAGAAAGACTTTAATACCAATGTGCTCAACGACACCACGGTCAAGGTGGGCCACGATGAGACCCTGACCGT
ACAGAACGCCCGCACCCGCACGGTCAAGGAAGGCGACGAAACCGTCACGCTGGAAAAGGGCAAGCGTAGCGTCGCCATCC
AGACCGGCAGCGACACGCTGGATGTCAAAGATACCCGCAGCGTGACGGTCGGTGGCGACCAGACCCACAGCACCGGCGGC
AATTACACTGACAAGGTCACCGGCAACTATAGCCTGACCGTGGACGGCAACCTGACCATCAAGGTCAGCGGCACCCTTAC
CCTGGAAAGCGGCGGCAGTTTCAGTATCAAGAGCGGTGCTGATCTGGCGACCCAGGCCAGCACTGCCATCAGCCACAAGG
CTGGAACATCGCTGGCCAATCAGGCGGGCACCACGCTGGAGAACAAAGCGGGAACCACCATGACCAACGACGCAGGCATC
AGCCTGATCAACAAGGCCAGCGCCTCACAGACCGTGGATGGCGGCGGCTTGCTGACCCTCAAGGGCGGTCTGGTAAAGGT
CAACTGA